##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BcgI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco52I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MslI MspA1I MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 1616 GAATTC [GA]^AATT[CT]
AcyI 1089 GGCGTC G[GA]^CG[CT]C
AocI 1793 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1629 AGCGCT AGC^GCT
ApoI 1616 GAATTC [GA]^AATT[CT]
BalI 1051 TGGCCA TGG^CCA
BamHI 1760 GGATCC G^GATCC
BbsI 1991 GTCTTC GAAGAC(2/6)
BciVI 1491 GTATCC GTATCC(6/5)
BpuAI 1991 GTCTTC GAAGAC(2/6)
BsaHI 1089 GGCGTC G[GA]^CG[CT]C
BsaI 720 GAGACC GGTCTC(1/5)
BsmBI 1586 GAGACG CGTCTC(1/5)
BssSI 629 CACGAG CACGAG(-5/-1)
Bsu36I 1793 CCTCAGG CC^T[ACGT]AGG
Eco47III 1629 AGCGCT AGC^GCT
Eco81I 1793 CCTCAGG CC^T[ACGT]AGG
EcoRI 1616 GAATTC G^AATTC
FauI 1530 GCGGG CCCGC(4/6)
HaeII 1629 AGCGCT [GA]GCGC^[CT]
HgaI 1090 GCGTC GACGC(5/10)
Hin1I 1089 GGCGTC G[GA]^CG[CT]C
MaeII 1489 ACGT A^CGT
MluNI 1051 TGGCCA TGG^CCA
MscI 1051 TGGCCA TGG^CCA
MseI 1380 TTAA T^TAA
NaeI 941 GCCGGC GCC^GGC
NgoMIV 941 GCCGGC G^CCGGC
NspI 1579 GCATGC [GA]CATG^[CT]
PaeR7I 1112 CTCGAG C^TCGAG
SphI 1579 GCATGC GCATG^C
TaqI 1113 TCGA T^CGA
Tru9I 1380 TTAA T^TAA
TthHB8I 1113 TCGA T^CGA
XcmI 1172 CCAACCCCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1112 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 26 GTAC GT^AC
1315 GTAC
Asp700I 1333 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1366 GAAGTCTTTC
BbvCI 1814 CCTCAGC CCTCAGC(-5/-2)
279 GCTGAGG
BlpI 517 GCTCAGC GC^T[ACGT]AGC
1010 GCTCAGC
Bpu1102I 517 GCTCAGC GC^T[ACGT]AGC
1010 GCTCAGC
BsrDI 223 GCAATG GCAATG(2/0)
1819 GCAATG
BsrFI 941 GCCGGC [GA]^CCGG[CT]
1287 ACCGGC
BstEII 108 GGTGACC G^GT[ACGT]ACC
1057 GGTAACC
BstPI 108 GGTGACC G^GT[ACGT]ACC
1057 GGTAACC
BstXI 697 CCAGGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1908 CCAGATGCTTGG
CelII 517 GCTCAGC GC^T[ACGT]AGC
1010 GCTCAGC
Cfr10I 941 GCCGGC [GA]^CCGG[CT]
1287 ACCGGC
DraIII 103 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1707 CACCCAGTG
EaeI 488 CGGCCA [CT]^GGCC[GA]
1051 TGGCCA
EcoNI 127 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
402 CCTCCAAAAGG
EcoO65I 108 GGTGACC G^GT[ACGT]ACC
1057 GGTAACC
PflMI 802 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1355 CCAGCCTGTGG
RsaI 26 GTAC GT^AC
1315 GTAC
SacI 973 GAGCTC GAGCT^C
1824 GAGCTC
SmaI 468 CCCGGG CCC^GGG
1422 CCCGGG
TfiI 657 GAATC G^A[AT]TC
1300 GAATC
Van91I 802 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1355 CCAGCCTGTGG
XmaCI 468 CCCGGG C^CCGGG
1422 CCCGGG
XmaI 468 CCCGGG C^CCGGG
1422 CCCGGG
XmnI 1333 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1366 GAAGTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TseI Tsp45I Tsp509I
TspRI XhoII