##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AspEI AspI
AvaI BamHI BanII BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BseRI BsiWI BsmAI BsmBI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DraIII DsaI Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FseI HaeII HgaI
Hin1I HindIII KasI KspI MfeI
MluI MroI MslI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 70 enzymes) #
###################################
Alw44I 338 GTGCAC G^TGCAC
AlwNI 686 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 338 GTGCAC G^TGCAC
AseI 436 ATTAAT AT^TAAT
AsnI 436 ATTAAT AT^TAAT
Asp700I 643 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 338 GTGCAC G[AT]GC[AT]^C
AvaII 820 GGACC G^G[AT]CC
AviII 16 TGCGCA TGC^GCA
AvrII 286 CCTAGG C^CTAGG
BalI 693 TGGCCA TGG^CCA
BbsI 559 GAAGAC GAAGAC(2/6)
BciVI 268 GGATAC GTATCC(6/5)
BclI 111 TGATCA T^GATCA
BglII 1005 AGATCT A^GATCT
BlnI 286 CCTAGG C^CTAGG
BmyI 338 GTGCAC G[AGT]GC[ACT]^C
BpmI 1103 CTGGAG CTGGAG(16/14)
BpuAI 559 GAAGAC GAAGAC(2/6)
BsaAI 1064 TACGTG [CT]AC^GT[GA]
BsaBI 796 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1281 CTGCAC GTGCAG(16/14)
BsiEI 26 CGGCCG CG[GA][CT]^CG
BsiHKAI 338 GTGCAC G[AT]GC[AT]^C
Bsp1286I 338 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 838 TGTACA T^GTACA
BspHI 372 TCATGA T^CATGA
BsrDI 1476 CATTGC GCAATG(2/0)
BsrGI 838 TGTACA T^GTACA
BstBI 1045 TTCGAA TT^CGAA
BstYI 1005 AGATCT [GA]^GATC[CT]
CfoI 17 GCGC GCG^C
Cfr13I 820 GGACC G^G[ACGT]CC
DrdI 239 GACTTGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 26 CGGCCG C^GGCCG
EarI 145 GAAGAG CTCTTC(1/4)
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
EcoRI 572 GAATTC G^AATTC
EcoT14I 286 CCTAGG C^C[AT][AT]GG
FbaI 111 TGATCA T^GATCA
FspI 16 TGCGCA TGC^GCA
HapII 25 CCGG C^CGG
HhaI 17 GCGC GCG^C
HinP1I 17 GCGC G^CGC
HpaII 25 CCGG C^CGG
Ksp632I 145 GAAGAG CTCTTC(1/4)
MamI 796 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1005 AGATCT [GA]^GATC[CT]
MluNI 693 TGGCCA TGG^CCA
MscI 693 TGGCCA TGG^CCA
MspA1I 1613 CAGCTG C[AC]G^C[GT]G
MspI 25 CCGG C^CGG
NspI 1465 GCATGT [GA]CATG^[CT]
NspV 1045 TTCGAA TT^CGAA
PshBI 436 ATTAAT AT^TAAT
PstI 1037 CTGCAG CTGCA^G
PvuII 1613 CAGCTG CAG^CTG
RcaI 372 TCATGA T^CATGA
Sau96I 820 GGACC G^G[ACGT]CC
SfcI 1037 CTGCAG C^T[GA][CT]AG
SfuI 1045 TTCGAA TT^CGAA
SmlI 1269 CTTGAG C^T[CT][GA]AG
SspBI 838 TGTACA T^GTACA
StuI 2139 AGGCCT AGG^CCT
StyI 286 CCTAGG C^C[AT][AT]GG
TaqI 1046 TCGA T^CGA
TthHB8I 1046 TCGA T^CGA
XhoII 1005 AGATCT [GA]^GATC[CT]
XmnI 643 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Acc65I 1856 GGTACC G^GTACC
2194 GGTACC
AccI 1346 GTAGAC GT^[AC][GT]AC
1963 GTCTAC
Asp718I 1856 GGTACC G^GTACC
2194 GGTACC
BsaJI 286 CCTAGG C^C[ACGT][ACGT]GG
1978 CCCTGG
BsmFI 915 GGGAC GGGAC(10/14)
922 GGGAC
BsmI 309 GAATGC GAATGC(1/-1)
1445 GAATGC
BspMI 323 ACCTGC ACCTGC(4/8)
1279 ACCTGC
BssKI 32 CCTGG ^CC[ACGT]GG
1979 CCTGG
BstNI 32 CCTGG CC^[AT]GG
1979 CCTGG
EaeI 26 CGGCCG [CT]^GGCC[GA]
693 TGGCCA
EcoRII 32 CCTGG ^CC[AT]GG
1979 CCTGG
HpaI 588 GTTAAC GTT^AAC
1820 GTTAAC
KpnI 1856 GGTACC GGTAC^C
2194 GGTACC
MvaI 32 CCTGG CC^[AT]GG
1979 CCTGG
ScrFI 32 CCTGG CC^[ACGT]GG
1979 CCTGG
SpeI 45 ACTAGT A^CTAGT
1421 ACTAGT
SspI 740 AATATT AAT^ATT
1503 AATATT
TfiI 216 GATTC G^A[AT]TC
796 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanI BbvI BfaI BsiYI
BslI BsrI BstF5I Cac8I DdeI
DpnII DraI Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI SfaNI
Tru9I TseI Tsp45I Tsp509I TspRI