##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BalI BbeI BbrPI
BbsI BfrI BglII BlnI BpuAI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FseI HgaI
Hin1I HpaI KasI KpnI KspI
MluI MluNI MroI MscI MseI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 339 GTCTAC GT^[AC][GT]AC
AflIII 86 ACATGT A^C[GA][CT]GT
Aor51HI 989 AGCGCT AGC^GCT
AspHI 1586 GAGCTC G[AT]GC[AT]^C
AviII 1271 TGCGCA TGC^GCA
BbvCI 1546 GCTGAGG CCTCAGC(-5/-2)
BclI 660 TGATCA T^GATCA
BlpI 59 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 59 GCTCAGC GC^T[ACGT]AGC
BsaBI 978 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1967 GAGGAG GAGGAG(10/8)
BsiHKAI 1586 GAGCTC G[AT]GC[AT]^C
Bsp1407I 1121 TGTACA T^GTACA
BspHI 1226 TCATGA T^CATGA
BspLU11I 86 ACATGT A^CATGT
BsrGI 1121 TGTACA T^GTACA
BstXI 1710 CCAAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 59 GCTCAGC GC^T[ACGT]AGC
EaeI 690 TGGCCG [CT]^GGCC[GA]
Eco47III 989 AGCGCT AGC^GCT
EcoRV 1651 GATATC GAT^ATC
FbaI 660 TGATCA T^GATCA
FspI 1271 TGCGCA TGC^GCA
HaeII 989 AGCGCT [GA]GCGC^[CT]
HincII 177 GTTGAC GT[CT]^[GA]AC
HindII 177 GTTGAC GT[CT]^[GA]AC
HindIII 137 AAGCTT A^AGCTT
MamI 978 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
RcaI 1226 TCATGA T^CATGA
SacI 1586 GAGCTC GAGCT^C
SapI 1833 GCTCTTC GCTCTTC(1/4)
ScaI 1741 AGTACT AGT^ACT
SfiI 717 GGCCTCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1456 CTCAAG C^T[CT][GA]AG
SpeI 1744 ACTAGT A^CTAGT
SspBI 1121 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 169 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
364 CAGGTTCTG
AvaI 311 CTCGGG C^[CT]CG[GA]G
905 CTCGGG
BamHI 1485 GGATCC G^GATCC
1676 GGATCC
BcgI 102 CGAGAGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1598 GCATTGGGCTCG
BciVI 585 GGATAC GTATCC(6/5)
1295 GGATAC
BpmI 871 CTCCAG CTGGAG(16/14)
232 CTGGAG
BsmI 414 GAATGC GAATGC(1/-1)
1617 GAATGC
BsoBI 311 CTCGGG C^[CT]CG[GA]G
905 CTCGGG
BspMI 321 ACCTGC ACCTGC(4/8)
112 GCAGGT
BstYI 1485 GGATCC [GA]^GATC[CT]
1676 GGATCC
EarI 1834 CTCTTC CTCTTC(1/4)
643 GAAGAG
Ksp632I 1834 CTCTTC CTCTTC(1/4)
643 GAAGAG
MaeII 15 ACGT A^CGT
1319 ACGT
MaeIII 184 GTGAC ^GT[ACGT]AC
433 GTGAC
MfeI 1090 CAATTG C^AATTG
2069 CAATTG
MflI 1485 GGATCC [GA]^GATC[CT]
1676 GGATCC
MunI 1090 CAATTG C^AATTG
2069 CAATTG
NspI 86 ACATGT [GA]CATG^[CT]
998 ACATGC
PpuMI 200 AGGTCCC [GA]G^G[AT]CC[CT]
744 GGGTCCC
PstI 1541 CTGCAG CTGCA^G
1553 CTGCAG
SfcI 1541 CTGCAG C^T[GA][CT]AG
1553 CTGCAG
TaqI 160 TCGA T^CGA
1590 TCGA
Tsp45I 184 GTGAC ^GT[GC]AC
433 GTGAC
TthHB8I 160 TCGA T^CGA
1590 TCGA
XhoII 1485 GGATCC [GA]^GATC[CT]
1676 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BsaI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI StuI StyI TfiI TseI
Tsp509I TspRI Van91I