##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AflII AflIII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII BbrPI BbvCI BcgI
BciVI BclI BfrI BlpI Bpu1102I
BsaBI BsiEI BsiWI BsoBI Bsp1407I
BspHI BspLU11I BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I CelII CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HincII HindII HpaI KpnI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
PvuI RcaI RsrII SacI SalI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 861 GTCTAC GT^[AC][GT]AC
AccIII 371 TCCGGA T^CCGGA
AclI 532 AACGTT AA^CGTT
Alw44I 181 GTGCAC G^TGCAC
AocI 781 CCTGAGG CC^T[ACGT]AGG
ApaLI 181 GTGCAC G^TGCAC
AvrII 1563 CCTAGG C^CTAGG
BamHI 507 GGATCC G^GATCC
BanII 435 GAGCCC G[GA]GC[CT]^C
BbsI 840 GTCTTC GAAGAC(2/6)
BfaI 1564 CTAG C^TAG
BglI 563 GCCCTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1733 AGATCT A^GATCT
BlnI 1563 CCTAGG C^CTAGG
BpuAI 840 GTCTTC GAAGAC(2/6)
BsaAI 82 TACGTG [CT]AC^GT[GA]
BsaWI 371 TCCGGA [AT]^CCGG[AT]
BseAI 371 TCCGGA T^CCGGA
BsmBI 627 CGTCTC CGTCTC(1/5)
BsmI 316 GCATTC GAATGC(1/-1)
BspDI 586 ATCGAT AT^CGAT
BspEI 371 TCCGGA T^CCGGA
BspMI 1209 GCAGGT ACCTGC(4/8)
BsrBI 1091 GAGCGG CCGCTC(-3/-3)
BsrFI 131 GCCGGC [GA]^CCGG[CT]
BssHII 1397 GCGCGC G^CGCGC
BssSI 449 CTCGTG CACGAG(-5/-1)
Bsu36I 781 CCTGAGG CC^T[ACGT]AGG
Cfr10I 131 GCCGGC [GA]^CCGG[CT]
ClaI 586 ATCGAT AT^CGAT
DraIII 149 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 781 CCTGAGG CC^T[ACGT]AGG
EcoRV 583 GATATC GAT^ATC
HindIII 1193 AAGCTT A^AGCTT
KspI 991 CCGCGG CCGC^GG
MaeI 1564 CTAG C^TAG
MroI 371 TCCGGA T^CCGGA
NaeI 131 GCCGGC GCC^GGC
NcoI 1025 CCATGG C^CATGG
NgoMIV 131 GCCGGC G^CCGGC
PshAI 621 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 532 AACGTT AA^CGTT
SacII 991 CCGCGG CCGC^GG
SapI 657 GAAGAGC GCTCTTC(1/4)
SbfI 1415 CCTGCAGG CCTGCA^GG
SexAI 1531 ACCTGGT A^CC[AT]GGT
SmlI 12 CTTGAG C^T[CT][GA]AG
Sse8387I 1415 CCTGCAGG CCTGCA^GG
TaqI 587 TCGA T^CGA
TthHB8I 587 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 992 CGCG CG^CG
1398 CGCG
AcsI 519 GAATTT [GA]^AATT[CT]
1757 AAATTT
ApoI 519 GAATTT [GA]^AATT[CT]
1757 AAATTT
BalI 71 TGGCCA TGG^CCA
400 TGGCCA
BcnI 297 CCGGG CC^[GC]GG
703 CCGGG
BsgI 240 GTGCAG GTGCAG(16/14)
452 GTGCAG
BsrDI 209 GCAATG GCAATG(2/0)
1646 GCAATG
BstAPI 242 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
677 GCAGCTGCTGC
BstUI 992 CGCG CG^CG
1398 CGCG
BstXI 424 CCAGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1595 CCAGTGTTGTGG
BstYI 507 GGATCC [GA]^GATC[CT]
1733 AGATCT
DraI 1480 TTTAAA TTT^AAA
1724 TTTAAA
DrdI 831 GACCCATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1487 GACTCATAGGTC
EaeI 71 TGGCCA [CT]^GGCC[GA]
400 TGGCCA
HgaI 245 GCGTC GACGC(5/10)
272 GCGTC
MflI 507 GGATCC [GA]^GATC[CT]
1733 AGATCT
MluNI 71 TGGCCA TGG^CCA
400 TGGCCA
MscI 71 TGGCCA TGG^CCA
400 TGGCCA
MslI 184 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1181 CATAGTTGTG
MvnI 992 CGCG CG^CG
1398 CGCG
NciI 297 CCGGG CC^[GC]GG
703 CCGGG
XcmI 421 CCACCAGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
725 CCACGCAGTCTCTGG
XhoII 507 GGATCC [GA]^GATC[CT]
1733 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BanI BbeI
BbvI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NarI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI