##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BbeI BcgI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaHI BsgI BsiEI BsiWI BspDI
BspHI BspLU11I BsrBI BsrDI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MscI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 41 CGCG CG^CG
AccIII 1878 TCCGGA T^CCGGA
AocI 2000 CCTGAGG CC^T[ACGT]AGG
Asp700I 361 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 164 CACGTG CAC^GTG
BbvCI 2117 GCTGAGG CCTCAGC(-5/-2)
BciVI 427 GTATCC GTATCC(6/5)
BclI 168 TGATCA T^GATCA
BglI 2190 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 828 AGATCT A^GATCT
BsaAI 164 CACGTG [CT]AC^GT[GA]
BsaI 2017 GAGACC GGTCTC(1/5)
BseAI 1878 TCCGGA T^CCGGA
BsmBI 104 GAGACG CGTCTC(1/5)
BsmI 1174 GCATTC GAATGC(1/-1)
Bsp1407I 1068 TGTACA T^GTACA
BspEI 1878 TCCGGA T^CCGGA
BsrGI 1068 TGTACA T^GTACA
BssSI 676 CACGAG CACGAG(-5/-1)
BstUI 41 CGCG CG^CG
BstXI 1967 CCAGCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2000 CCTGAGG CC^T[ACGT]AGG
DraI 697 TTTAAA TTT^AAA
DsaI 1946 CCGTGG C^C[GA][CT]GG
EaeI 661 CGGCCA [CT]^GGCC[GA]
Eco81I 2000 CCTGAGG CC^T[ACGT]AGG
EcoRI 501 GAATTC G^AATTC
EcoT14I 252 CCAAGG C^C[AT][AT]GG
FbaI 168 TGATCA T^GATCA
HgaI 442 GACGC GACGC(5/10)
HincII 1283 GTCAAC GT[CT]^[GA]AC
HindII 1283 GTCAAC GT[CT]^[GA]AC
MroI 1878 TCCGGA T^CCGGA
MvnI 41 CGCG CG^CG
NspI 2086 GCATGC [GA]CATG^[CT]
PmaCI 164 CACGTG CAC^GTG
PmeI 696 GTTTAAAC GTTT^AAAC
PmlI 164 CACGTG CAC^GTG
SacI 24 GAGCTC GAGCT^C
ScaI 954 AGTACT AGT^ACT
SfaNI 1665 GCATC GCATC(5/9)
SmaI 2141 CCCGGG CCC^GGG
SphI 2086 GCATGC GCATG^C
SspBI 1068 TGTACA T^GTACA
StyI 252 CCAAGG C^C[AT][AT]GG
XmaCI 2141 CCCGGG C^CCGGG
XmaI 2141 CCCGGG C^CCGGG
XmnI 361 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 501 GAATTC [GA]^AATT[CT]
1043 AAATTT
AflIII 915 ACGTGT A^C[GA][CT]GT
1708 ACACGT
Alw44I 245 GTGCAC G^TGCAC
314 GTGCAC
AlwNI 1225 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
1858 CAGCTGCTG
Aor51HI 1163 AGCGCT AGC^GCT
1969 AGCGCT
ApaLI 245 GTGCAC G^TGCAC
314 GTGCAC
ApoI 501 GAATTC [GA]^AATT[CT]
1043 AAATTT
BbsI 541 GAAGAC GAAGAC(2/6)
998 GTCTTC
BpuAI 541 GAAGAC GAAGAC(2/6)
998 GTCTTC
BspMI 1441 ACCTGC ACCTGC(4/8)
2092 ACCTGC
BsrFI 67 GCCGGC [GA]^CCGG[CT]
1776 GCCGGC
Cfr10I 67 GCCGGC [GA]^CCGG[CT]
1776 GCCGGC
DraII 588 AGGCCCC [GA]G^G[ACGT]CC[CT]
1658 GGGGCCT
EarI 16 GAAGAG CTCTTC(1/4)
584 GAAGAG
Eco47III 1163 AGCGCT AGC^GCT
1969 AGCGCT
EcoO109I 588 AGGCCCC [GA]G^G[ACGT]CC[CT]
1658 GGGGCCT
Ksp632I 16 GAAGAG CTCTTC(1/4)
584 GAAGAG
NaeI 67 GCCGGC GCC^GGC
1776 GCCGGC
NgoMIV 67 GCCGGC G^CCGGC
1776 GCCGGC
PflMI 147 CCATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1828 CCAGGACATGG
Van91I 147 CCATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1828 CCAGGACATGG
XcmI 570 CCATCGAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1467 CCAGGCTGCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BamHI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII