################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AgeI AhdI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbeI BcgI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsgI BsiEI BsiWI BspDI BspHI BspLU11I BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 41 CGCG CG^CG AccIII 1878 TCCGGA T^CCGGA AocI 2000 CCTGAGG CC^T[ACGT]AGG Asp700I 361 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 164 CACGTG CAC^GTG BbvCI 2117 GCTGAGG CCTCAGC(-5/-2) BciVI 427 GTATCC GTATCC(6/5) BclI 168 TGATCA T^GATCA BglI 2190 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 828 AGATCT A^GATCT BsaAI 164 CACGTG [CT]AC^GT[GA] BsaI 2017 GAGACC GGTCTC(1/5) BseAI 1878 TCCGGA T^CCGGA BsmBI 104 GAGACG CGTCTC(1/5) BsmI 1174 GCATTC GAATGC(1/-1) Bsp1407I 1068 TGTACA T^GTACA BspEI 1878 TCCGGA T^CCGGA BsrGI 1068 TGTACA T^GTACA BssSI 676 CACGAG CACGAG(-5/-1) BstUI 41 CGCG CG^CG BstXI 1967 CCAGCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2000 CCTGAGG CC^T[ACGT]AGG DraI 697 TTTAAA TTT^AAA DsaI 1946 CCGTGG C^C[GA][CT]GG EaeI 661 CGGCCA [CT]^GGCC[GA] Eco81I 2000 CCTGAGG CC^T[ACGT]AGG EcoRI 501 GAATTC G^AATTC EcoT14I 252 CCAAGG C^C[AT][AT]GG FbaI 168 TGATCA T^GATCA HgaI 442 GACGC GACGC(5/10) HincII 1283 GTCAAC GT[CT]^[GA]AC HindII 1283 GTCAAC GT[CT]^[GA]AC MroI 1878 TCCGGA T^CCGGA MvnI 41 CGCG CG^CG NspI 2086 GCATGC [GA]CATG^[CT] PmaCI 164 CACGTG CAC^GTG PmeI 696 GTTTAAAC GTTT^AAAC PmlI 164 CACGTG CAC^GTG SacI 24 GAGCTC GAGCT^C ScaI 954 AGTACT AGT^ACT SfaNI 1665 GCATC GCATC(5/9) SmaI 2141 CCCGGG CCC^GGG SphI 2086 GCATGC GCATG^C SspBI 1068 TGTACA T^GTACA StyI 252 CCAAGG C^C[AT][AT]GG XmaCI 2141 CCCGGG C^CCGGG XmaI 2141 CCCGGG C^CCGGG XmnI 361 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 501 GAATTC [GA]^AATT[CT] 1043 AAATTT AflIII 915 ACGTGT A^C[GA][CT]GT 1708 ACACGT Alw44I 245 GTGCAC G^TGCAC 314 GTGCAC AlwNI 1225 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG 1858 CAGCTGCTG Aor51HI 1163 AGCGCT AGC^GCT 1969 AGCGCT ApaLI 245 GTGCAC G^TGCAC 314 GTGCAC ApoI 501 GAATTC [GA]^AATT[CT] 1043 AAATTT BbsI 541 GAAGAC GAAGAC(2/6) 998 GTCTTC BpuAI 541 GAAGAC GAAGAC(2/6) 998 GTCTTC BspMI 1441 ACCTGC ACCTGC(4/8) 2092 ACCTGC BsrFI 67 GCCGGC [GA]^CCGG[CT] 1776 GCCGGC Cfr10I 67 GCCGGC [GA]^CCGG[CT] 1776 GCCGGC DraII 588 AGGCCCC [GA]G^G[ACGT]CC[CT] 1658 GGGGCCT EarI 16 GAAGAG CTCTTC(1/4) 584 GAAGAG Eco47III 1163 AGCGCT AGC^GCT 1969 AGCGCT EcoO109I 588 AGGCCCC [GA]G^G[ACGT]CC[CT] 1658 GGGGCCT Ksp632I 16 GAAGAG CTCTTC(1/4) 584 GAAGAG NaeI 67 GCCGGC GCC^GGC 1776 GCCGGC NgoMIV 67 GCCGGC G^CCGGC 1776 GCCGGC PflMI 147 CCATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1828 CCAGGACATGG Van91I 147 CCATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1828 CCAGGACATGG XcmI 570 CCATCGAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1467 CCAGGCTGCCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BamHI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII