##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BbrPI BbvCI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 1242 GGCGCC G[GA]^CG[CT]C
AlwNI 511 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1284 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 18 GAGCTC G[AT]GC[AT]^C
AspI 507 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1550 GGACC G^G[AT]CC
AviII 1616 TGCGCA TGC^GCA
BalI 1651 TGGCCA TGG^CCA
BbeI 1242 GGCGCC GGCGC^C
BcgI 15 CGAGAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 529 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 529 GCTCAGC GC^T[ACGT]AGC
BsaHI 1242 GGCGCC G[GA]^CG[CT]C
BsiHKAI 18 GAGCTC G[AT]GC[AT]^C
BsmBI 977 GAGACG CGTCTC(1/5)
BsrBI 1161 GAGCGG CCGCTC(-3/-3)
BstXI 857 CCACTCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 529 GCTCAGC GC^T[ACGT]AGC
DraI 396 TTTAAA TTT^AAA
DsaI 991 CCATGG C^C[GA][CT]GG
EcoNI 1589 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1616 TGCGCA TGC^GCA
HaeII 1242 GGCGCC [GA]GCGC^[CT]
Hin1I 1242 GGCGCC G[GA]^CG[CT]C
KasI 1242 GGCGCC G^GCGCC
MaeII 653 ACGT A^CGT
MluNI 1651 TGGCCA TGG^CCA
MscI 1651 TGGCCA TGG^CCA
NarI 1242 GGCGCC GG^CGCC
NcoI 991 CCATGG C^CATGG
NheI 534 GCTAGC G^CTAGC
NspI 1870 GCATGT [GA]CATG^[CT]
PflFI 507 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1595 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 165 CGATCG CGAT^CG
SacI 18 GAGCTC GAGCT^C
ScaI 816 AGTACT AGT^ACT
SfoI 1242 GGCGCC GGC^GCC
SspI 228 AATATT AAT^ATT
TfiI 1392 GATTC G^A[AT]TC
Tth111I 507 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1595 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1537 CCATTTCCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1284 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 11 GATCC GGATC(4/5)
1697 GATCC
AvaI 909 CCCGAG C^[CT]CG[GA]G
1692 CCCGAG
BciVI 833 GGATAC GTATCC(6/5)
254 GTATCC
BsgI 649 CTGCAC GTGCAG(16/14)
709 GTGCAG
BsoBI 909 CCCGAG C^[CT]CG[GA]G
1692 CCCGAG
BspMI 647 ACCTGC ACCTGC(4/8)
1851 ACCTGC
BsrDI 1264 GCAATG GCAATG(2/0)
1599 GCAATG
BssSI 71 CACGAG CACGAG(-5/-1)
1401 CACGAG
BstYI 10 AGATCC [GA]^GATC[CT]
1696 AGATCC
EaeI 994 TGGCCG [CT]^GGCC[GA]
1651 TGGCCA
FauI 80 GCGGG CCCGC(4/6)
1163 GCGGG
MflI 10 AGATCC [GA]^GATC[CT]
1696 AGATCC
MseI 397 TTAA T^TAA
1969 TTAA
MspA1I 78 CAGCGG C[AC]G^C[GT]G
1865 CCGCTG
PstI 517 CTGCAG CTGCA^G
1949 CTGCAG
TaqI 1097 TCGA T^CGA
1292 TCGA
Tru9I 397 TTAA T^TAA
1969 TTAA
TthHB8I 1097 TCGA T^CGA
1292 TCGA
XhoII 10 AGATCC [GA]^GATC[CT]
1696 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
Tsp509I TspRI