##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AviII
AvrII BamHI BbrPI BbvCI BciVI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BsrDI
BsrFI BssHII Bst1107I BstBI BstZ17I
CelII Cfr10I ClaI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KspI MamI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI SacII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 1812 CGCG CG^CG
AcyI 2114 GGCGCC G[GA]^CG[CT]C
AflIII 1538 ACGTGT A^C[GA][CT]GT
Asp700I 413 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 257 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2114 GGCGCC GGCGC^C
BbsI 1513 GAAGAC GAAGAC(2/6)
BcgI 74 CGATGTGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 821 TGATCA T^GATCA
BfaI 231 CTAG C^TAG
BpuAI 1513 GAAGAC GAAGAC(2/6)
BsaHI 2114 GGCGCC G[GA]^CG[CT]C
BsaWI 324 TCCGGT [AT]^CCGG[AT]
BsgI 100 GTGCAG GTGCAG(16/14)
Bsp1407I 1198 TGTACA T^GTACA
BspHI 818 TCATGA T^CATGA
BsrGI 1198 TGTACA T^GTACA
BssSI 1426 CTCGTG CACGAG(-5/-1)
BstAPI 1546 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 110 GGTTACC G^GT[ACGT]ACC
BstPI 110 GGTTACC G^GT[ACGT]ACC
BstUI 1812 CGCG CG^CG
CpoI 249 CGGTCCG CG^G[AT]CCG
DrdI 38 GACTTCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 110 GGTTACC G^GT[ACGT]ACC
FbaI 821 TGATCA T^GATCA
HaeII 2114 GGCGCC [GA]GCGC^[CT]
HgaI 1665 GACGC GACGC(5/10)
Hin1I 2114 GGCGCC G[GA]^CG[CT]C
HincII 2022 GTTGAC GT[CT]^[GA]AC
HindII 2022 GTTGAC GT[CT]^[GA]AC
KasI 2114 GGCGCC G^GCGCC
MaeI 231 CTAG C^TAG
MvnI 1812 CGCG CG^CG
NarI 2114 GGCGCC GG^CGCC
NspI 876 ACATGC [GA]CATG^[CT]
PflFI 257 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 818 TCATGA T^CATGA
RsrII 249 CGGTCCG CG^G[AT]CCG
SacI 1423 GAGCTC GAGCT^C
SapI 2254 GCTCTTC GCTCTTC(1/4)
SexAI 26 ACCTGGT A^CC[AT]GGT
SfoI 2114 GGCGCC GGC^GCC
SmaI 1871 CCCGGG CCC^GGG
SspBI 1198 TGTACA T^GTACA
StuI 587 AGGCCT AGG^CCT
TaqI 980 TCGA T^CGA
Tth111I 257 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 980 TCGA T^CGA
XmaCI 1871 CCCGGG C^CCGGG
XmaI 1871 CCCGGG C^CCGGG
XmnI 413 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Acc65I 217 GGTACC G^GTACC
1790 GGTACC
AcsI 1828 GAATTT [GA]^AATT[CT]
2437 GAATTT
AocI 195 CCTGAGG CC^T[ACGT]AGG
590 CCTGAGG
ApoI 1828 GAATTT [GA]^AATT[CT]
2437 GAATTT
Asp718I 217 GGTACC G^GTACC
1790 GGTACC
BalI 897 TGGCCA TGG^CCA
2351 TGGCCA
BglII 481 AGATCT A^GATCT
1767 AGATCT
BsaI 23 GAGACC GGTCTC(1/5)
1566 GGTCTC
Bsu36I 195 CCTGAGG CC^T[ACGT]AGG
590 CCTGAGG
DraIII 1646 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2007 CACGCAGTG
DsaI 305 CCGTGG C^C[GA][CT]GG
463 CCACGG
Eco81I 195 CCTGAGG CC^T[ACGT]AGG
590 CCTGAGG
KpnI 217 GGTACC GGTAC^C
1790 GGTACC
MluNI 897 TGGCCA TGG^CCA
2351 TGGCCA
MscI 897 TGGCCA TGG^CCA
2351 TGGCCA
PflMI 479 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2070 CCACTGGCTGG
SmlI 1714 CTCAAG C^T[CT][GA]AG
1771 CTCAAG
Van91I 479 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2070 CCACTGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII