################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AviII AvrII BamHI BbrPI BbvCI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrDI BsrFI BssHII Bst1107I BstBI BstZ17I CelII Cfr10I ClaI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KspI MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 1812 CGCG CG^CG AcyI 2114 GGCGCC G[GA]^CG[CT]C AflIII 1538 ACGTGT A^C[GA][CT]GT Asp700I 413 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 257 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 2114 GGCGCC GGCGC^C BbsI 1513 GAAGAC GAAGAC(2/6) BcgI 74 CGATGTGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 821 TGATCA T^GATCA BfaI 231 CTAG C^TAG BpuAI 1513 GAAGAC GAAGAC(2/6) BsaHI 2114 GGCGCC G[GA]^CG[CT]C BsaWI 324 TCCGGT [AT]^CCGG[AT] BsgI 100 GTGCAG GTGCAG(16/14) Bsp1407I 1198 TGTACA T^GTACA BspHI 818 TCATGA T^CATGA BsrGI 1198 TGTACA T^GTACA BssSI 1426 CTCGTG CACGAG(-5/-1) BstAPI 1546 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 110 GGTTACC G^GT[ACGT]ACC BstPI 110 GGTTACC G^GT[ACGT]ACC BstUI 1812 CGCG CG^CG CpoI 249 CGGTCCG CG^G[AT]CCG DrdI 38 GACTTCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 110 GGTTACC G^GT[ACGT]ACC FbaI 821 TGATCA T^GATCA HaeII 2114 GGCGCC [GA]GCGC^[CT] HgaI 1665 GACGC GACGC(5/10) Hin1I 2114 GGCGCC G[GA]^CG[CT]C HincII 2022 GTTGAC GT[CT]^[GA]AC HindII 2022 GTTGAC GT[CT]^[GA]AC KasI 2114 GGCGCC G^GCGCC MaeI 231 CTAG C^TAG MvnI 1812 CGCG CG^CG NarI 2114 GGCGCC GG^CGCC NspI 876 ACATGC [GA]CATG^[CT] PflFI 257 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 818 TCATGA T^CATGA RsrII 249 CGGTCCG CG^G[AT]CCG SacI 1423 GAGCTC GAGCT^C SapI 2254 GCTCTTC GCTCTTC(1/4) SexAI 26 ACCTGGT A^CC[AT]GGT SfoI 2114 GGCGCC GGC^GCC SmaI 1871 CCCGGG CCC^GGG SspBI 1198 TGTACA T^GTACA StuI 587 AGGCCT AGG^CCT TaqI 980 TCGA T^CGA Tth111I 257 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 980 TCGA T^CGA XmaCI 1871 CCCGGG C^CCGGG XmaI 1871 CCCGGG C^CCGGG XmnI 413 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Acc65I 217 GGTACC G^GTACC 1790 GGTACC AcsI 1828 GAATTT [GA]^AATT[CT] 2437 GAATTT AocI 195 CCTGAGG CC^T[ACGT]AGG 590 CCTGAGG ApoI 1828 GAATTT [GA]^AATT[CT] 2437 GAATTT Asp718I 217 GGTACC G^GTACC 1790 GGTACC BalI 897 TGGCCA TGG^CCA 2351 TGGCCA BglII 481 AGATCT A^GATCT 1767 AGATCT BsaI 23 GAGACC GGTCTC(1/5) 1566 GGTCTC Bsu36I 195 CCTGAGG CC^T[ACGT]AGG 590 CCTGAGG DraIII 1646 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 2007 CACGCAGTG DsaI 305 CCGTGG C^C[GA][CT]GG 463 CCACGG Eco81I 195 CCTGAGG CC^T[ACGT]AGG 590 CCTGAGG KpnI 217 GGTACC GGTAC^C 1790 GGTACC MluNI 897 TGGCCA TGG^CCA 2351 TGGCCA MscI 897 TGGCCA TGG^CCA 2351 TGGCCA PflMI 479 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2070 CCACTGGCTGG SmlI 1714 CTCAAG C^T[CT][GA]AG 1771 CTCAAG Van91I 479 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2070 CCACTGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII