##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
BbsI BciVI BfrI BpuAI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
ClaI CpoI DraI DraIII Eam1105I
Eco47III EcoNI EcoT22I FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NcoI NdeI NheI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI SwaI XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 71 enzymes) #
###################################
AccI 1850 GTAGAC GT^[AC][GT]AC
AcsI 1760 GAATTC [GA]^AATT[CT]
AcyI 648 GGCGCC G[GA]^CG[CT]C
Alw44I 870 GTGCAC G^TGCAC
AocI 777 CCTGAGG CC^T[ACGT]AGG
ApaLI 870 GTGCAC G^TGCAC
ApoI 1760 GAATTC [GA]^AATT[CT]
AspI 570 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1451 CCTAGG C^CTAGG
BamHI 1912 GGATCC G^GATCC
BanI 648 GGCGCC G^G[CT][GA]CC
BbeI 648 GGCGCC GGCGC^C
BbvCI 1604 CCTCAGC CCTCAGC(-5/-2)
BclI 326 TGATCA T^GATCA
BfaI 1452 CTAG C^TAG
BglI 1267 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1162 AGATCT A^GATCT
BlnI 1451 CCTAGG C^CTAGG
BlpI 1042 GCTCAGC GC^T[ACGT]AGC
BpmI 706 CTGGAG CTGGAG(16/14)
Bpu1102I 1042 GCTCAGC GC^T[ACGT]AGC
BsaHI 648 GGCGCC G[GA]^CG[CT]C
BseRI 57 GAGGAG GAGGAG(10/8)
BsgI 307 GTGCAG GTGCAG(16/14)
BsiEI 1253 CGGCCG CG[GA][CT]^CG
Bsp1407I 1234 TGTACA T^GTACA
BspHI 1523 TCATGA T^CATGA
BsrDI 1153 GCAATG GCAATG(2/0)
BsrFI 1047 GCCGGC [GA]^CCGG[CT]
BsrGI 1234 TGTACA T^GTACA
BstEII 128 GGTCACC G^GT[ACGT]ACC
BstPI 128 GGTCACC G^GT[ACGT]ACC
Bsu36I 777 CCTGAGG CC^T[ACGT]AGG
CelII 1042 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1047 GCCGGC [GA]^CCGG[CT]
DrdI 695 GACGCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1253 CGGCCG C^GGCCG
EclXI 1253 CGGCCG C^GGCCG
Eco52I 1253 CGGCCG C^GGCCG
Eco81I 777 CCTGAGG CC^T[ACGT]AGG
EcoO65I 128 GGTCACC G^GT[ACGT]ACC
EcoRI 1760 GAATTC G^AATTC
EcoRV 1500 GATATC GAT^ATC
FbaI 326 TGATCA T^GATCA
HaeII 648 GGCGCC [GA]GCGC^[CT]
HgaI 695 GACGC GACGC(5/10)
Hin1I 648 GGCGCC G[GA]^CG[CT]C
KasI 648 GGCGCC G^GCGCC
MaeI 1452 CTAG C^TAG
MseI 1943 TTAA T^TAA
NaeI 1047 GCCGGC GCC^GGC
NarI 648 GGCGCC GG^CGCC
NgoMIV 1047 GCCGGC G^CCGGC
NotI 1252 GCGGCCGC GC^GGCCGC
NspI 331 ACATGC [GA]CATG^[CT]
PaeR7I 1597 CTCGAG C^TCGAG
PflFI 570 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1124 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1523 TCATGA T^CATGA
SacI 989 GAGCTC GAGCT^C
SapI 909 GAAGAGC GCTCTTC(1/4)
SbfI 857 CCTGCAGG CCTGCA^GG
SexAI 1129 ACCTGGT A^CC[AT]GGT
SfoI 648 GGCGCC GGC^GCC
Sse8387I 857 CCTGCAGG CCTGCA^GG
SspBI 1234 TGTACA T^GTACA
StuI 506 AGGCCT AGG^CCT
Tru9I 1943 TTAA T^TAA
Tth111I 570 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1124 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1597 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 943 GGGCCC GGGCC^C
1318 GGGCCC
AvaI 1283 CTCGGG C^[CT]CG[GA]G
1597 CTCGAG
AviII 193 TGCGCA TGC^GCA
796 TGCGCA
BbrPI 189 CACGTG CAC^GTG
228 CACGTG
BsaI 360 GAGACC GGTCTC(1/5)
1119 GAGACC
BsoBI 1283 CTCGGG C^[CT]CG[GA]G
1597 CTCGAG
BsrBI 1040 CCGCTC CCGCTC(-3/-3)
1517 CCGCTC
BstXI 364 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1055 CCACCCCAGTGG
EarI 909 GAAGAG CTCTTC(1/4)
1888 GAAGAG
FspI 193 TGCGCA TGC^GCA
796 TGCGCA
Ksp632I 909 GAAGAG CTCTTC(1/4)
1888 GAAGAG
MboII 909 GAAGA GAAGA(8/7)
1888 GAAGA
PmaCI 189 CACGTG CAC^GTG
228 CACGTG
PmlI 189 CACGTG CAC^GTG
228 CACGTG
PstI 759 CTGCAG CTGCA^G
858 CTGCAG
PvuII 1331 CAGCTG CAG^CTG
1546 CAGCTG
SmlI 491 CTTGAG C^T[CT][GA]AG
1597 CTCGAG
TaqI 1027 TCGA T^CGA
1598 TCGA
Tsp509I 849 AATT ^AATT
1761 AATT
TthHB8I 1027 TCGA T^CGA
1598 TCGA
XcmI 139 CCACGGGACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1610 CCACAAGTACGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaII BalI BanII
BbvI BcgI BcnI BmyI BsaAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp45I TspRI XhoII