##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI BamHI BbeI
BbsI BcgI BciVI BclI BfrI
BglII BlpI Bpu1102I BpuAI BsaBI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI HincII
HindII HpaI KasI KpnI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tsp509I Tth111I XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 1052 GGCGTC G[GA]^CG[CT]C
AocI 1232 CCTGAGG CC^T[ACGT]AGG
AvrII 255 CCTAGG C^CTAGG
BanI 299 GGCACC G^G[CT][GA]CC
BbrPI 731 CACGTG CAC^GTG
BbvCI 1979 CCTCAGC CCTCAGC(-5/-2)
BlnI 255 CCTAGG C^CTAGG
BsaAI 731 CACGTG [CT]AC^GT[GA]
BsaHI 1052 GGCGTC G[GA]^CG[CT]C
BsaI 1581 GAGACC GGTCTC(1/5)
BsmBI 2044 GAGACG CGTCTC(1/5)
BsrDI 1031 GCAATG GCAATG(2/0)
BstAPI 1651 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1232 CCTGAGG CC^T[ACGT]AGG
DrdI 2046 GACGTGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1232 CCTGAGG CC^T[ACGT]AGG
EcoNI 1377 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 1864 GGCCGGCC GGCCGG^CC
Hin1I 1052 GGCGTC G[GA]^CG[CT]C
HindIII 2076 AAGCTT A^AGCTT
MseI 2073 TTAA T^TAA
PaeR7I 890 CTCGAG C^TCGAG
PflMI 1426 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 731 CACGTG CAC^GTG
PmlI 731 CACGTG CAC^GTG
PstI 28 CTGCAG CTGCA^G
PvuII 602 CAGCTG CAG^CTG
SapI 704 GAAGAGC GCTCTTC(1/4)
Tru9I 2073 TTAA T^TAA
Van91I 1426 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 890 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 482 GATCC GGATC(4/5)
1969 GATCC
AlwNI 1290 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2036 CAGAAGCTG
Aor51HI 61 AGCGCT AGC^GCT
1618 AGCGCT
Asp700I 704 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
845 GAAGCCCTTC
BglI 496 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1344 GCCGCCCCGGC
BseRI 1113 GAGGAG GAGGAG(10/8)
1533 GAGGAG
BsgI 1285 CTGCAC GTGCAG(16/14)
1649 CTGCAC
BsmI 1467 GAATGC GAATGC(1/-1)
1849 GAATGC
BstF5I 721 CATCC GGATG(2/0)
746 CATCC
BstYI 481 AGATCC [GA]^GATC[CT]
1968 AGATCC
DpnII 482 GATC ^GATC
1969 GATC
Eco47III 61 AGCGCT AGC^GCT
1618 AGCGCT
FokI 721 CATCC GGATG(9/13)
746 CATCC
HinfI 1407 GAGTC G^A[ACGT]TC
1962 GACTC
KspI 244 CCGCGG CCGC^GG
1270 CCGCGG
MboI 482 GATC ^GATC
1969 GATC
MflI 481 AGATCC [GA]^GATC[CT]
1968 AGATCC
NcoI 13 CCATGG C^CATGG
1804 CCATGG
NdeII 482 GATC ^GATC
1969 GATC
NotI 364 GCGGCCGC GC^GGCCGC
868 GCGGCCGC
PleI 1962 GACTC GAGTC(4/5)
1407 GAGTC
SacI 633 GAGCTC GAGCT^C
1632 GAGCTC
SacII 244 CCGCGG CCGC^GG
1270 CCGCGG
Sau3AI 482 GATC ^GATC
1969 GATC
SfaNI 1476 GATGC GCATC(5/9)
745 GCATC
SfcI 28 CTGCAG C^T[GA][CT]AG
599 CTACAG
Tsp45I 1036 GTGAC ^GT[GC]AC
2049 GTGAC
XhoII 481 AGATCC [GA]^GATC[CT]
1968 AGATCC
XmnI 704 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
845 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
ApaLI AspHI AvaI AvaII AviII
BalI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssHII BssKI BssSI BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FspI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIII NlaIV NspI PpuMI
RsaI Sau96I ScrFI SgrAI SmlI
StuI StyI TaqI TseI TspRI
TthHB8I