##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI AscI AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BfrI BglII BlnI BsaAI BsaHI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI Tth111I
Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 1280 GGTACC G^GTACC
Alw44I 738 GTGCAC G^TGCAC
AlwNI 1473 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 738 GTGCAC G^TGCAC
Asp718I 1280 GGTACC G^GTACC
AspHI 738 GTGCAC G[AT]GC[AT]^C
BanI 1280 GGTACC G^G[CT][GA]CC
BbsI 968 GAAGAC GAAGAC(2/6)
BcgI 880 CGAAGTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 811 GTATCC GTATCC(6/5)
BclI 959 TGATCA T^GATCA
BglI 95 GCCCTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 908 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 908 GCTGAGC GC^T[ACGT]AGC
BpuAI 968 GAAGAC GAAGAC(2/6)
BsiEI 895 CGATCG CG[GA][CT]^CG
BsiHKAI 738 GTGCAC G[AT]GC[AT]^C
Bsp1407I 1427 TGTACA T^GTACA
BspHI 373 TCATGA T^CATGA
BspMI 37 GCAGGT ACCTGC(4/8)
BsrBI 21 GAGCGG CCGCTC(-3/-3)
BsrGI 1427 TGTACA T^GTACA
BstXI 1285 CCAGTATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 468 GGATCT [GA]^GATC[CT]
CelII 908 GCTGAGC GC^T[ACGT]AGC
CfoI 344 GCGC GCG^C
DrdI 1198 GACACTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1034 GAATTC G^AATTC
EcoT14I 277 CCAAGG C^C[AT][AT]GG
FauI 905 CCCGC CCCGC(4/6)
FbaI 959 TGATCA T^GATCA
HaeII 343 AGCGCC [GA]GCGC^[CT]
HhaI 344 GCGC GCG^C
HinP1I 344 GCGC G^CGC
KpnI 1280 GGTACC GGTAC^C
MflI 468 GGATCT [GA]^GATC[CT]
MwoI 95 GCCCTCGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PacI 1597 TTAATTAA TTAAT^TAA
PpuMI 309 GGGTCCT [GA]G^G[AT]CC[CT]
PvuI 895 CGATCG CGAT^CG
RcaI 373 TCATGA T^CATGA
SfiI 94 GGCCCTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 89 CCCGGG CCC^GGG
SmlI 221 CTCAAG C^T[CT][GA]AG
SpeI 1023 ACTAGT A^CTAGT
SphI 1668 GCATGC GCATG^C
SspBI 1427 TGTACA T^GTACA
StuI 2061 AGGCCT AGG^CCT
StyI 277 CCAAGG C^C[AT][AT]GG
TaqI 745 TCGA T^CGA
Tsp45I 1040 GTGAC ^GT[GC]AC
TthHB8I 745 TCGA T^CGA
XbaI 1173 TCTAGA T^CTAGA
XhoII 468 GGATCT [GA]^GATC[CT]
XmaCI 89 CCCGGG C^CCGGG
XmaI 89 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AseI 1606 ATTAAT AT^TAAT
1968 ATTAAT
AsnI 1606 ATTAAT AT^TAAT
1968 ATTAAT
Asp700I 377 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1894 GAAGAGGTTC
AvaI 89 CCCGGG C^[CT]CG[GA]G
98 CTCGGG
AvaII 66 GGTCC G^G[AT]CC
310 GGTCC
BpmI 627 CTGGAG CTGGAG(16/14)
1479 CTGGAG
BsaI 40 GGTCTC GGTCTC(1/5)
512 GGTCTC
BsaWI 11 TCCGGT [AT]^CCGG[AT]
82 ACCGGA
BsgI 140 GTGCAG GTGCAG(16/14)
414 GTGCAG
BsoBI 89 CCCGGG C^[CT]CG[GA]G
98 CTCGGG
EarI 486 GAAGAG CTCTTC(1/4)
1894 GAAGAG
EcoT22I 1980 ATGCAT ATGCA^T
2267 ATGCAT
HphI 693 GGTGA GGTGA(8/7)
1758 GGTGA
Ksp632I 486 GAAGAG CTCTTC(1/4)
1894 GAAGAG
MslI 2103 CACATAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2105 CATAAATGTG
NsiI 1980 ATGCAT ATGCA^T
2267 ATGCAT
PleI 219 GACTC GAGTC(4/5)
1296 GAGTC
PshBI 1606 ATTAAT AT^TAAT
1968 ATTAAT
PstI 388 CTGCAG CTGCA^G
481 CTGCAG
PvuII 507 CAGCTG CAG^CTG
624 CAGCTG
SspI 1214 AATATT AAT^ATT
1422 AATATT
TfiI 1073 GATTC G^A[AT]TC
2114 GAATC
XmnI 377 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1894 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApaI ApoI BalI BanII
BbvI BcnI BfaI BmyI BsaBI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeI MaeII
MaeIII MamI MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI NciI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI Tru9I TseI Tsp509I
TspRI