##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaHI BsaI BseRI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 11 CGCG CG^CG
AcsI 1299 AAATTT [GA]^AATT[CT]
ApoI 1299 AAATTT [GA]^AATT[CT]
AspHI 270 GAGCTC G[AT]GC[AT]^C
AvaI 15 CCCGGG C^[CT]CG[GA]G
AviII 234 TGCGCA TGC^GCA
BanII 270 GAGCTC G[GA]GC[CT]^C
BfaI 1107 CTAG C^TAG
BglI 932 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 270 GAGCTC G[AGT]GC[ACT]^C
BsiHKAI 270 GAGCTC G[AT]GC[AT]^C
BsmAI 874 GAGAC GTCTC(1/5)
BsoBI 15 CCCGGG C^[CT]CG[GA]G
Bsp1286I 270 GAGCTC G[AGT]GC[ACT]^C
BsrDI 928 CATTGC GCAATG(2/0)
BstUI 11 CGCG CG^CG
EcoNI 30 CCTGCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 58 CCTTGG C^C[AT][AT]GG
FauI 322 CCCGC CCCGC(4/6)
FspI 234 TGCGCA TGC^GCA
HaeII 217 GGCGCT [GA]GCGC^[CT]
HgaI 1141 GACGC GACGC(5/10)
MaeI 1107 CTAG C^TAG
MaeII 646 ACGT A^CGT
MvnI 11 CGCG CG^CG
NspI 520 GCATGT [GA]CATG^[CT]
PstI 671 CTGCAG CTGCA^G
SacI 270 GAGCTC GAGCT^C
ScaI 549 AGTACT AGT^ACT
SexAI 84 ACCTGGT A^CC[AT]GGT
SfcI 671 CTGCAG C^T[GA][CT]AG
SmaI 15 CCCGGG CCC^GGG
SspI 791 AATATT AAT^ATT
StyI 58 CCTTGG C^C[AT][AT]GG
TaqI 872 TCGA T^CGA
TthHB8I 872 TCGA T^CGA
XmaCI 15 CCCGGG C^CCGGG
XmaI 15 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 96 TCCGGA T^CCGGA
1137 TCCGGA
AfaI 550 GTAC GT^AC
1237 GTAC
BalI 61 TGGCCA TGG^CCA
924 TGGCCA
BcnI 15 CCCGG CC^[GC]GG
16 CCGGG
BlpI 145 GCTCAGC GC^T[ACGT]AGC
779 GCTAAGC
BpmI 404 CTGGAG CTGGAG(16/14)
1281 CTGGAG
Bpu1102I 145 GCTCAGC GC^T[ACGT]AGC
779 GCTAAGC
BsaWI 96 TCCGGA [AT]^CCGG[AT]
1137 TCCGGA
BseAI 96 TCCGGA T^CCGGA
1137 TCCGGA
BsgI 839 CTGCAC GTGCAG(16/14)
806 GTGCAG
BspEI 96 TCCGGA T^CCGGA
1137 TCCGGA
BstXI 300 CCATCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
651 CCAACTCTATGG
CelII 145 GCTCAGC GC^T[ACGT]AGC
779 GCTAAGC
DraII 318 AGGTCCC [GA]G^G[ACGT]CC[CT]
1420 GGGACCT
EcoO109I 318 AGGTCCC [GA]G^G[ACGT]CC[CT]
1420 GGGACCT
MluNI 61 TGGCCA TGG^CCA
924 TGGCCA
MroI 96 TCCGGA T^CCGGA
1137 TCCGGA
MscI 61 TGGCCA TGG^CCA
924 TGGCCA
NciI 15 CCCGG CC^[GC]GG
16 CCGGG
NlaIV 319 GGTCCC GG[ACGT]^[ACGT]CC
1420 GGGACC
PpuMI 318 AGGTCCC [GA]G^G[AT]CC[CT]
1420 GGGACCT
RsaI 550 GTAC GT^AC
1237 GTAC
SfaNI 121 GATGC GCATC(5/9)
1019 GATGC
SmlI 428 CTTGAG C^T[CT][GA]AG
735 CTCAAG
Tsp509I 580 AATT ^AATT
1300 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BbsI BbvI BpuAI BsaJI BsiYI
BslI BsmFI BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII PflMI PleI
PvuII Sau3AI Sau96I ScrFI TfiI
Tru9I TseI Tsp45I TspRI Van91I
XcmI