################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SapI SbfI SexAI SfiI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tsp509I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 319 GTCGAC GT^[AC][GT]AC AflIII 1878 ACGTGT A^C[GA][CT]GT Alw44I 730 GTGCAC G^TGCAC AlwNI 1811 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1131 CCTCAGG CC^T[ACGT]AGG Aor51HI 511 AGCGCT AGC^GCT ApaLI 730 GTGCAC G^TGCAC AspHI 730 GTGCAC G[AT]GC[AT]^C AspI 1813 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 974 GGCGCC G^G[CT][GA]CC BbeI 974 GGCGCC GGCGC^C BbrPI 1877 CACGTG CAC^GTG BbvCI 1397 CCTCAGC CCTCAGC(-5/-2) BfaI 1176 CTAG C^TAG BlpI 1490 GCTGAGC GC^T[ACGT]AGC BpmI 1808 CTCCAG CTGGAG(16/14) Bpu1102I 1490 GCTGAGC GC^T[ACGT]AGC BsaAI 1877 CACGTG [CT]AC^GT[GA] BsaI 1674 GAGACC GGTCTC(1/5) BsiHKAI 730 GTGCAC G[AT]GC[AT]^C BsmAI 1674 GAGAC GTCTC(1/5) BsmI 909 GAATGC GAATGC(1/-1) BsrBI 45 GAGCGG CCGCTC(-3/-3) BsrDI 989 GCAATG GCAATG(2/0) BsrI 858 CCAGT ACTGG(1/-1) BssSI 536 CACGAG CACGAG(-5/-1) BstAPI 762 GCAAGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 369 AGATCC [GA]^GATC[CT] Bsu36I 1131 CCTCAGG CC^T[ACGT]AGG CelII 1490 GCTGAGC GC^T[ACGT]AGC Eco47III 511 AGCGCT AGC^GCT Eco81I 1131 CCTCAGG CC^T[ACGT]AGG HincII 319 GTCGAC GT[CT]^[GA]AC HindII 319 GTCGAC GT[CT]^[GA]AC KasI 974 GGCGCC G^GCGCC KspI 1302 CCGCGG CCGC^GG MaeI 1176 CTAG C^TAG MflI 369 AGATCC [GA]^GATC[CT] NaeI 1651 GCCGGC GCC^GGC NarI 974 GGCGCC GG^CGCC NgoMIV 1651 GCCGGC G^CCGGC NotI 1109 GCGGCCGC GC^GGCCGC PflFI 1813 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1877 CACGTG CAC^GTG PmlI 1877 CACGTG CAC^GTG PpuMI 393 AGGACCT [GA]G^G[AT]CC[CT] PstI 1313 CTGCAG CTGCA^G SacII 1302 CCGCGG CCGC^GG SalI 319 GTCGAC G^TCGAC ScaI 783 AGTACT AGT^ACT SfcI 1313 CTGCAG C^T[GA][CT]AG SfoI 974 GGCGCC GGC^GCC SgrAI 1650 CGCCGGCG C[GA]^CCGG[CT]G Tsp45I 1825 GTGAC ^GT[GC]AC Tth111I 1813 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 369 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 974 GGCGCC G[GA]^CG[CT]C 1628 GACGCC ApaI 746 GGGCCC GGGCC^C 1436 GGGCCC BbsI 544 GAAGAC GAAGAC(2/6) 1803 GAAGAC BpuAI 544 GAAGAC GAAGAC(2/6) 1803 GAAGAC BsaHI 974 GGCGCC G[GA]^CG[CT]C 1628 GACGCC BsiEI 566 CGGCCG CG[GA][CT]^CG 1110 CGGCCG BsmFI 1485 GGGAC GGGAC(10/14) 1663 GTCCC BspMI 1240 ACCTGC ACCTGC(4/8) 1346 GCAGGT BssHII 528 GCGCGC G^CGCGC 1548 GCGCGC BstXI 858 CCAGTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1474 CCAGGAGTTTGG EagI 566 CGGCCG C^GGCCG 1110 CGGCCG EclXI 566 CGGCCG C^GGCCG 1110 CGGCCG Eco52I 566 CGGCCG C^GGCCG 1110 CGGCCG EcoT14I 948 CCATGG C^C[AT][AT]GG 1047 CCATGG Hin1I 974 GGCGCC G[GA]^CG[CT]C 1628 GACGCC MseI 296 TTAA T^TAA 1716 TTAA NcoI 948 CCATGG C^CATGG 1047 CCATGG SmlI 398 CTCAAG C^T[CT][GA]AG 1601 CTCAAG SphI 465 GCATGC GCATG^C 660 GCATGC StyI 948 CCATGG C^C[AT][AT]GG 1047 CCATGG Tru9I 296 TTAA T^TAA 1716 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AfaI AluI AlwI Asp700I AvaI AvaII BalI BanII BbvI BcnI BglI BmyI BsaJI BseRI BsgI BsiYI BslI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmaI StuI TaqI TseI TspRI TthHB8I XmaCI XmaI XmnI