##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BcgI BciVI BclI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SapI SbfI SexAI
SfiI SfuI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tsp509I
Van91I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 319 GTCGAC GT^[AC][GT]AC
AflIII 1878 ACGTGT A^C[GA][CT]GT
Alw44I 730 GTGCAC G^TGCAC
AlwNI 1811 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1131 CCTCAGG CC^T[ACGT]AGG
Aor51HI 511 AGCGCT AGC^GCT
ApaLI 730 GTGCAC G^TGCAC
AspHI 730 GTGCAC G[AT]GC[AT]^C
AspI 1813 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 974 GGCGCC G^G[CT][GA]CC
BbeI 974 GGCGCC GGCGC^C
BbrPI 1877 CACGTG CAC^GTG
BbvCI 1397 CCTCAGC CCTCAGC(-5/-2)
BfaI 1176 CTAG C^TAG
BlpI 1490 GCTGAGC GC^T[ACGT]AGC
BpmI 1808 CTCCAG CTGGAG(16/14)
Bpu1102I 1490 GCTGAGC GC^T[ACGT]AGC
BsaAI 1877 CACGTG [CT]AC^GT[GA]
BsaI 1674 GAGACC GGTCTC(1/5)
BsiHKAI 730 GTGCAC G[AT]GC[AT]^C
BsmAI 1674 GAGAC GTCTC(1/5)
BsmI 909 GAATGC GAATGC(1/-1)
BsrBI 45 GAGCGG CCGCTC(-3/-3)
BsrDI 989 GCAATG GCAATG(2/0)
BsrI 858 CCAGT ACTGG(1/-1)
BssSI 536 CACGAG CACGAG(-5/-1)
BstAPI 762 GCAAGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 369 AGATCC [GA]^GATC[CT]
Bsu36I 1131 CCTCAGG CC^T[ACGT]AGG
CelII 1490 GCTGAGC GC^T[ACGT]AGC
Eco47III 511 AGCGCT AGC^GCT
Eco81I 1131 CCTCAGG CC^T[ACGT]AGG
HincII 319 GTCGAC GT[CT]^[GA]AC
HindII 319 GTCGAC GT[CT]^[GA]AC
KasI 974 GGCGCC G^GCGCC
KspI 1302 CCGCGG CCGC^GG
MaeI 1176 CTAG C^TAG
MflI 369 AGATCC [GA]^GATC[CT]
NaeI 1651 GCCGGC GCC^GGC
NarI 974 GGCGCC GG^CGCC
NgoMIV 1651 GCCGGC G^CCGGC
NotI 1109 GCGGCCGC GC^GGCCGC
PflFI 1813 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1877 CACGTG CAC^GTG
PmlI 1877 CACGTG CAC^GTG
PpuMI 393 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1313 CTGCAG CTGCA^G
SacII 1302 CCGCGG CCGC^GG
SalI 319 GTCGAC G^TCGAC
ScaI 783 AGTACT AGT^ACT
SfcI 1313 CTGCAG C^T[GA][CT]AG
SfoI 974 GGCGCC GGC^GCC
SgrAI 1650 CGCCGGCG C[GA]^CCGG[CT]G
Tsp45I 1825 GTGAC ^GT[GC]AC
Tth111I 1813 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 369 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 974 GGCGCC G[GA]^CG[CT]C
1628 GACGCC
ApaI 746 GGGCCC GGGCC^C
1436 GGGCCC
BbsI 544 GAAGAC GAAGAC(2/6)
1803 GAAGAC
BpuAI 544 GAAGAC GAAGAC(2/6)
1803 GAAGAC
BsaHI 974 GGCGCC G[GA]^CG[CT]C
1628 GACGCC
BsiEI 566 CGGCCG CG[GA][CT]^CG
1110 CGGCCG
BsmFI 1485 GGGAC GGGAC(10/14)
1663 GTCCC
BspMI 1240 ACCTGC ACCTGC(4/8)
1346 GCAGGT
BssHII 528 GCGCGC G^CGCGC
1548 GCGCGC
BstXI 858 CCAGTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1474 CCAGGAGTTTGG
EagI 566 CGGCCG C^GGCCG
1110 CGGCCG
EclXI 566 CGGCCG C^GGCCG
1110 CGGCCG
Eco52I 566 CGGCCG C^GGCCG
1110 CGGCCG
EcoT14I 948 CCATGG C^C[AT][AT]GG
1047 CCATGG
Hin1I 974 GGCGCC G[GA]^CG[CT]C
1628 GACGCC
MseI 296 TTAA T^TAA
1716 TTAA
NcoI 948 CCATGG C^CATGG
1047 CCATGG
SmlI 398 CTCAAG C^T[CT][GA]AG
1601 CTCAAG
SphI 465 GCATGC GCATG^C
660 GCATGC
StyI 948 CCATGG C^C[AT][AT]GG
1047 CCATGG
Tru9I 296 TTAA T^TAA
1716 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
Asp700I AvaI AvaII BalI BanII
BbvI BcnI BglI BmyI BsaJI
BseRI BsgI BsiYI BslI BsoBI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmaI StuI
TaqI TseI TspRI TthHB8I XmaCI
XmaI XmnI