##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbrPI BlnI BlpI Bpu1102I
BsaAI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BsrBI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraIII DrdI Eam1105I Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HgaI HincII HindII HpaI
KpnI MfeI MroI MslI MunI
NcoI NdeI NheI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 1882 GAATTT [GA]^AATT[CT]
AflII 1894 CTTAAG C^TTAAG
AlwNI 2124 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 222 GGGCCC GGGCC^C
ApoI 1882 GAATTT [GA]^AATT[CT]
Asp700I 2086 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 311 GAGCTC G[AT]GC[AT]^C
BamHI 355 GGATCC G^GATCC
BbsI 875 GAAGAC GAAGAC(2/6)
BcgI 1067 CGACTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1902 GGATAC GTATCC(6/5)
BclI 2219 TGATCA T^GATCA
BfrI 1894 CTTAAG C^TTAAG
BglII 1871 AGATCT A^GATCT
BpuAI 875 GAAGAC GAAGAC(2/6)
BsaBI 954 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2137 ACCGGA [AT]^CCGG[AT]
BsiHKAI 311 GAGCTC G[AT]GC[AT]^C
BspLU11I 675 ACATGT A^CATGT
BssHII 183 GCGCGC G^CGCGC
DraI 1912 TTTAAA TTT^AAA
EarI 410 GAAGAG CTCTTC(1/4)
EcoT14I 1212 CCTTGG C^C[AT][AT]GG
FbaI 2219 TGATCA T^GATCA
HindIII 1302 AAGCTT A^AGCTT
Ksp632I 410 GAAGAG CTCTTC(1/4)
MamI 954 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1240 ACGCGT A^CGCGT
PstI 1424 CTGCAG CTGCA^G
SacI 311 GAGCTC GAGCT^C
SfiI 214 GGCCACCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 500 CCCGGG CCC^GGG
SspI 2310 AATATT AAT^ATT
StyI 1212 CCTTGG C^C[AT][AT]GG
XcmI 540 CCAAAGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 500 CCCGGG C^CCGGG
XmaI 500 CCCGGG C^CCGGG
XmnI 2086 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 679 GTAGAC GT^[AC][GT]AC
1984 GTAGAC
AflIII 675 ACATGT A^C[GA][CT]GT
1240 ACGCGT
BsaI 1376 GGTCTC GGTCTC(1/5)
1708 GGTCTC
BspHI 603 TCATGA T^CATGA
1414 TCATGA
BstXI 111 CCACCGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1222 CCAAAGTGCTGG
BstYI 355 GGATCC [GA]^GATC[CT]
1871 AGATCT
MaeII 623 ACGT A^CGT
648 ACGT
MflI 355 GGATCC [GA]^GATC[CT]
1871 AGATCT
NotI 54 GCGGCCGC GC^GGCCGC
158 GCGGCCGC
PpuMI 31 GGGTCCC [GA]G^G[AT]CC[CT]
2207 AGGTCCC
RcaI 603 TCATGA T^CATGA
1414 TCATGA
ScaI 1858 AGTACT AGT^ACT
1941 AGTACT
SmlI 1894 CTTAAG C^T[CT][GA]AG
2251 CTTGAG
SphI 1108 GCATGC GCATG^C
1637 GCATGC
StuI 766 AGGCCT AGG^CCT
2037 AGGCCT
TaqI 1182 TCGA T^CGA
1712 TCGA
TthHB8I 1182 TCGA T^CGA
1712 TCGA
XhoII 355 GGATCC [GA]^GATC[CT]
1871 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI AspI AvaI AvaII
BalI BanI BanII BbeI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I Eco81I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KspI MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PleI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I