##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BfrI BglII BlnI BsaBI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoRI EcoRV EcoT22I HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PstI PvuI
PvuII RsrII SalI SbfI ScaI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XcmI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcyI 82 GGCGCC G[GA]^CG[CT]C
AspHI 1692 GAGCTC G[AT]GC[AT]^C
AviII 1247 TGCGCA TGC^GCA
BamHI 1365 GGATCC G^GATCC
BbeI 82 GGCGCC GGCGC^C
BbrPI 1699 CACGTG CAC^GTG
BbsI 1567 GTCTTC GAAGAC(2/6)
BbvCI 1411 GCTGAGG CCTCAGC(-5/-2)
BcgI 1029 GCACCAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 898 GGATAC GTATCC(6/5)
BclI 1352 TGATCA T^GATCA
BlpI 624 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 624 GCTGAGC GC^T[ACGT]AGC
BpuAI 1567 GTCTTC GAAGAC(2/6)
BsaAI 1699 CACGTG [CT]AC^GT[GA]
BsaHI 82 GGCGCC G[GA]^CG[CT]C
BsaWI 2153 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1692 GAGCTC G[AT]GC[AT]^C
BsmBI 612 GAGACG CGTCTC(1/5)
BspHI 804 TCATGA T^CATGA
BstXI 1668 CCAAATAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1365 GGATCC [GA]^GATC[CT]
CelII 624 GCTGAGC GC^T[ACGT]AGC
DraIII 1619 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 1352 TGATCA T^GATCA
FseI 29 GGCCGGCC GGCCGG^CC
FspI 1247 TGCGCA TGC^GCA
Hin1I 82 GGCGCC G[GA]^CG[CT]C
HincII 2321 GTTGAC GT[CT]^[GA]AC
HindII 2321 GTTGAC GT[CT]^[GA]AC
KasI 82 GGCGCC G^GCGCC
KspI 35 CCGCGG CCGC^GG
MaeII 1700 ACGT A^CGT
MflI 1365 GGATCC [GA]^GATC[CT]
NarI 82 GGCGCC GG^CGCC
NcoI 2302 CCATGG C^CATGG
PaeR7I 1790 CTCGAG C^TCGAG
PmaCI 1699 CACGTG CAC^GTG
PmlI 1699 CACGTG CAC^GTG
PshAI 1062 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 804 TCATGA T^CATGA
SacI 1692 GAGCTC GAGCT^C
SacII 35 CCGCGG CCGC^GG
SapI 1470 GAAGAGC GCTCTTC(1/4)
SexAI 1838 ACCTGGT A^CC[AT]GGT
SfiI 967 GGCCCAGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 82 GGCGCC GGC^GCC
SmaI 89 CCCGGG CCC^GGG
SphI 1850 GCATGC GCATG^C
TfiI 1731 GAATC G^A[AT]TC
XhoI 1790 CTCGAG C^TCGAG
XhoII 1365 GGATCC [GA]^GATC[CT]
XmaCI 89 CCCGGG C^CCGGG
XmaI 89 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 1320 GAATTT [GA]^AATT[CT]
1558 GAATTT
AocI 97 CCTGAGG CC^T[ACGT]AGG
933 CCTGAGG
ApoI 1320 GAATTT [GA]^AATT[CT]
1558 GAATTT
BsaI 1717 GAGACC GGTCTC(1/5)
1294 GGTCTC
BseRI 1182 CTCCTC GAGGAG(10/8)
1421 GAGGAG
BsgI 793 CTGCAC GTGCAG(16/14)
836 GTGCAG
BstEII 1123 GGTAACC G^GT[ACGT]ACC
1654 GGTTACC
BstPI 1123 GGTAACC G^GT[ACGT]ACC
1654 GGTTACC
Bsu36I 97 CCTGAGG CC^T[ACGT]AGG
933 CCTGAGG
Eco81I 97 CCTGAGG CC^T[ACGT]AGG
933 CCTGAGG
EcoNI 582 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2019 CCTCCCAGAGG
EcoO65I 1123 GGTAACC G^GT[ACGT]ACC
1654 GGTTACC
HgaI 614 GACGC GACGC(5/10)
184 GCGTC
MseI 1646 TTAA T^TAA
2424 TTAA
NspI 1850 GCATGC [GA]CATG^[CT]
1862 ACATGC
PpuMI 937 AGGACCC [GA]G^G[AT]CC[CT]
2199 AGGACCT
SmlI 1790 CTCGAG C^T[CT][GA]AG
2278 CTTGAG
TaqI 1419 TCGA T^CGA
1791 TCGA
Tru9I 1646 TTAA T^TAA
2424 TTAA
TthHB8I 1419 TCGA T^CGA
1791 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TseI Tsp45I Tsp509I
TspRI Van91I