##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BciVI BclI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FbaI FspI HindIII HpaI
KasI MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII ScaI
SexAI SfoI SgrAI SnaBI SpeI
SphI SspBI SwaI TfiI Tth111I
Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 520 GGTACC G^GTACC
AccI 16 GTCGAC GT^[AC][GT]AC
AcsI 165 GAATTC [GA]^AATT[CT]
AcyI 306 GACGCC G[GA]^CG[CT]C
AflII 1408 CTTAAG C^TTAAG
AflIII 1194 ACGTGT A^C[GA][CT]GT
AlwNI 639 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 672 CCTGAGG CC^T[ACGT]AGG
ApaI 1103 GGGCCC GGGCC^C
ApoI 165 GAATTC [GA]^AATT[CT]
Asp700I 161 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 520 GGTACC G^GTACC
BbrPI 1193 CACGTG CAC^GTG
BbsI 608 GAAGAC GAAGAC(2/6)
BfrI 1408 CTTAAG C^TTAAG
BpuAI 608 GAAGAC GAAGAC(2/6)
BsaAI 1193 CACGTG [CT]AC^GT[GA]
BsaHI 306 GACGCC G[GA]^CG[CT]C
BseRI 957 CTCCTC GAGGAG(10/8)
BsgI 911 GTGCAG GTGCAG(16/14)
BsmAI 1460 GAGAC GTCTC(1/5)
BsrFI 1187 GCCGGC [GA]^CCGG[CT]
BsrI 255 CCAGT ACTGG(1/-1)
BssSI 231 CTCGTG CACGAG(-5/-1)
BstAPI 227 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 363 TTCGAA TT^CGAA
BstYI 589 AGATCC [GA]^GATC[CT]
Bsu36I 672 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1187 GCCGGC [GA]^CCGG[CT]
DraI 1585 TTTAAA TTT^AAA
Eco81I 672 CCTGAGG CC^T[ACGT]AGG
EcoRI 165 GAATTC G^AATTC
FseI 1186 GGCCGGCC GGCCGG^CC
Hin1I 306 GACGCC G[GA]^CG[CT]C
KpnI 520 GGTACC GGTAC^C
KspI 1139 CCGCGG CCGC^GG
MaeII 1194 ACGT A^CGT
MflI 589 AGATCC [GA]^GATC[CT]
NaeI 1187 GCCGGC GCC^GGC
NcoI 121 CCATGG C^CATGG
NgoMIV 1187 GCCGGC G^CCGGC
NspV 363 TTCGAA TT^CGAA
PmaCI 1193 CACGTG CAC^GTG
PmlI 1193 CACGTG CAC^GTG
PshAI 1462 GACTAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1139 CCGCGG CCGC^GG
SalI 16 GTCGAC G^TCGAC
SbfI 644 CCTGCAGG CCTGCA^GG
SfiI 428 GGCCACGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 363 TTCGAA TT^CGAA
Sse8387I 644 CCTGCAGG CCTGCA^GG
SspI 1579 AATATT AAT^ATT
StuI 1084 AGGCCT AGG^CCT
XcmI 255 CCAGTAGGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 589 AGATCC [GA]^GATC[CT]
XmnI 161 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 765 GTGCAC G^TGCAC
1056 GTGCAC
AlwI 590 GATCC GGATC(4/5)
269 GGATC
ApaLI 765 GTGCAC G^TGCAC
1056 GTGCAC
BalI 464 TGGCCA TGG^CCA
1493 TGGCCA
BspMI 325 ACCTGC ACCTGC(4/8)
551 ACCTGC
BsrBI 1222 CCGCTC CCGCTC(-3/-3)
744 GAGCGG
HaeII 112 AGCGCC [GA]GCGC^[CT]
1129 AGCGCC
HgaI 306 GACGC GACGC(5/10)
1307 GCGTC
HincII 16 GTCGAC GT[CT]^[GA]AC
714 GTCAAC
HindII 16 GTCGAC GT[CT]^[GA]AC
714 GTCAAC
HphI 605 GGTGA GGTGA(8/7)
1149 GGTGA
MaeIII 1336 GTGAC ^GT[ACGT]AC
1532 GTCAC
MluNI 464 TGGCCA TGG^CCA
1493 TGGCCA
MscI 464 TGGCCA TGG^CCA
1493 TGGCCA
PpuMI 1390 GGGACCC [GA]G^G[AT]CC[CT]
1467 GGGTCCC
SacI 480 GAGCTC GAGCT^C
579 GAGCTC
Tsp45I 1336 GTGAC ^GT[GC]AC
1532 GTCAC
Tsp509I 166 AATT ^AATT
1004 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
BsmI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeI MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
Tru9I TseI TspRI TthHB8I XmaCI
XmaI