##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
KpnI KspI MaeI MaeII MluI
MroI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 1175 GGCGCC G[GA]^CG[CT]C
AflIII 966 ACATGT A^C[GA][CT]GT
Alw44I 1717 GTGCAC G^TGCAC
AocI 989 CCTGAGG CC^T[ACGT]AGG
ApaLI 1717 GTGCAC G^TGCAC
BalI 939 TGGCCA TGG^CCA
BbeI 1175 GGCGCC GGCGC^C
BbsI 697 GAAGAC GAAGAC(2/6)
BcgI 1489 CGAGGACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1648 CCGGG CC^[GC]GG
BglI 1562 GCCTCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 697 GAAGAC GAAGAC(2/6)
BsaBI 628 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1175 GGCGCC G[GA]^CG[CT]C
BsaI 1094 GAGACC GGTCTC(1/5)
BspHI 1224 TCATGA T^CATGA
BspLU11I 966 ACATGT A^CATGT
Bsu36I 989 CCTGAGG CC^T[ACGT]AGG
DraI 1800 TTTAAA TTT^AAA
DraII 166 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 1198 GACAGAGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 989 CCTGAGG CC^T[ACGT]AGG
EcoO109I 166 GGGTCCC [GA]G^G[ACGT]CC[CT]
HaeII 1175 GGCGCC [GA]GCGC^[CT]
HapII 1648 CCGG C^CGG
Hin1I 1175 GGCGCC G[GA]^CG[CT]C
HincII 443 GTTAAC GT[CT]^[GA]AC
HindII 443 GTTAAC GT[CT]^[GA]AC
HpaI 443 GTTAAC GTT^AAC
HpaII 1648 CCGG C^CGG
KasI 1175 GGCGCC G^GCGCC
MamI 628 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 840 CAATTG C^AATTG
MluNI 939 TGGCCA TGG^CCA
MscI 939 TGGCCA TGG^CCA
MspI 1648 CCGG C^CGG
MunI 840 CAATTG C^AATTG
NarI 1175 GGCGCC GG^CGCC
NciI 1648 CCGGG CC^[GC]GG
PflMI 1691 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 813 GAGTC GAGTC(4/5)
PpuMI 166 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1224 TCATGA T^CATGA
ScaI 948 AGTACT AGT^ACT
SfoI 1175 GGCGCC GGC^GCC
SphI 1447 GCATGC GCATG^C
StuI 1365 AGGCCT AGG^CCT
SwaI 1799 ATTTAAAT ATTT^AAAT
Van91I 1691 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 284 GTAGAC GT^[AC][GT]AC
1475 GTAGAC
Asp700I 559 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
697 GAAGACTTTC
AvaI 659 CTCGAG C^[CT]CG[GA]G
776 CTCGAG
AvaII 167 GGTCC G^G[AT]CC
1206 GGTCC
BbvCI 143 GCTGAGG CCTCAGC(-5/-2)
308 GCTGAGG
BpmI 897 CTGGAG CTGGAG(16/14)
1790 CTGGAG
BseRI 992 GAGGAG GAGGAG(10/8)
1193 GAGGAG
BsmAI 1094 GAGAC GTCTC(1/5)
1196 GAGAC
BsmI 1048 GAATGC GAATGC(1/-1)
689 GCATTC
BsoBI 659 CTCGAG C^[CT]CG[GA]G
776 CTCGAG
BspMI 1302 ACCTGC ACCTGC(4/8)
364 GCAGGT
BsrDI 289 CATTGC GCAATG(2/0)
1495 CATTGC
BstYI 885 AGATCC [GA]^GATC[CT]
997 GGATCT
CfoI 1176 GCGC GCG^C
1550 GCGC
HhaI 1176 GCGC GCG^C
1550 GCGC
HinP1I 1176 GCGC G^CGC
1550 GCGC
MflI 885 AGATCC [GA]^GATC[CT]
997 GGATCT
MslI 328 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1279 CATTGAGATG
PaeR7I 659 CTCGAG C^TCGAG
776 CTCGAG
SacI 535 GAGCTC GAGCT^C
773 GAGCTC
XhoI 659 CTCGAG C^TCGAG
776 CTCGAG
XhoII 885 AGATCC [GA]^GATC[CT]
997 GGATCT
XmnI 559 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
697 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI BanI BanII BbvI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HindIII HinfI HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI