##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BanI BanII BbeI BbvCI
BclI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspEI BspLU11I
BsrDI BssHII BstAPI BstBI BstEII
BstPI CelII CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 1687 CGCG CG^CG
AcsI 367 AAATTC [GA]^AATT[CT]
AocI 1477 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1406 AGCGCT AGC^GCT
ApoI 367 AAATTC [GA]^AATT[CT]
AvaI 1759 CCCGGG C^[CT]CG[GA]G
BamHI 99 GGATCC G^GATCC
BbrPI 112 CACGTG CAC^GTG
BciVI 1259 GTATCC GTATCC(6/5)
BglI 349 GCCAGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 648 AGATCT A^GATCT
BsaAI 112 CACGTG [CT]AC^GT[GA]
BsaI 622 GAGACC GGTCTC(1/5)
BsgI 1483 GTGCAG GTGCAG(16/14)
BsoBI 1759 CCCGGG C^[CT]CG[GA]G
Bsp1407I 119 TGTACA T^GTACA
BspDI 58 ATCGAT AT^CGAT
BsrFI 1430 ACCGGC [GA]^CCGG[CT]
BsrGI 119 TGTACA T^GTACA
BsrI 209 ACTGG ACTGG(1/-1)
BssSI 301 CACGAG CACGAG(-5/-1)
Bst1107I 802 GTATAC GTA^TAC
BstUI 1687 CGCG CG^CG
BstZ17I 802 GTATAC GTA^TAC
Bsu36I 1477 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1430 ACCGGC [GA]^CCGG[CT]
ClaI 58 ATCGAT AT^CGAT
EaeI 1469 TGGCCG [CT]^GGCC[GA]
EarI 580 GAAGAG CTCTTC(1/4)
Eco47III 1406 AGCGCT AGC^GCT
Eco81I 1477 CCTGAGG CC^T[ACGT]AGG
HgaI 539 GCGTC GACGC(5/10)
HincII 193 GTCGAC GT[CT]^[GA]AC
HindII 193 GTCGAC GT[CT]^[GA]AC
HphI 1628 GGTGA GGTGA(8/7)
Ksp632I 580 GAAGAG CTCTTC(1/4)
KspI 1686 CCGCGG CCGC^GG
MaeII 113 ACGT A^CGT
MvnI 1687 CGCG CG^CG
NdeI 905 CATATG CA^TATG
NspI 386 ACATGC [GA]CATG^[CT]
PmaCI 112 CACGTG CAC^GTG
PmlI 112 CACGTG CAC^GTG
PpuMI 1538 AGGACCC [GA]G^G[AT]CC[CT]
SacII 1686 CCGCGG CCGC^GG
SalI 193 GTCGAC G^TCGAC
SmaI 1759 CCCGGG CCC^GGG
SspBI 119 TGTACA T^GTACA
StuI 1729 AGGCCT AGG^CCT
TfiI 1569 GAATC G^A[AT]TC
Tsp45I 1499 GTGAC ^GT[GC]AC
XbaI 243 TCTAGA T^CTAGA
XmaCI 1759 CCCGGG C^CCGGG
XmaI 1759 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 193 GTCGAC GT^[AC][GT]AC
802 GTATAC
AlwNI 670 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1594 CAGAAGCTG
Asp700I 375 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
646 GAAGATCTTC
AspHI 985 GAGCAC G[AT]GC[AT]^C
1003 GTGCTC
BbsI 880 GAAGAC GAAGAC(2/6)
1765 GAAGAC
BcgI 1198 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
185 GCAAGACTGTCG
BfaI 244 CTAG C^TAG
1363 CTAG
BpuAI 880 GAAGAC GAAGAC(2/6)
1765 GAAGAC
BsiHKAI 985 GAGCAC G[AT]GC[AT]^C
1003 GTGCTC
BsmFI 1121 GGGAC GGGAC(10/14)
1682 GGGAC
BspMI 1783 ACCTGC ACCTGC(4/8)
6 GCAGGT
BsrBI 1048 GAGCGG CCGCTC(-3/-3)
1703 GAGCGG
DraII 38 AGGCCCT [GA]G^G[ACGT]CC[CT]
1538 AGGACCC
EcoO109I 38 AGGCCCT [GA]G^G[ACGT]CC[CT]
1538 AGGACCC
HaeII 1359 GGCGCT [GA]GCGC^[CT]
1406 AGCGCT
MaeI 244 CTAG C^TAG
1363 CTAG
MaeIII 53 GTAAC ^GT[ACGT]AC
1499 GTGAC
NcoI 1340 CCATGG C^CATGG
1466 CCATGG
PleI 1372 GACTC GAGTC(4/5)
1776 GACTC
SmlI 1006 CTCAAG C^T[CT][GA]AG
1240 CTCAAG
XmnI 375 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
646 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BspHI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII