##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwNI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BlnI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstEII
BstPI BstUI BstXI Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI FseI FspI HaeII
HgaI Hin1I HphI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AciI 134 GCGG CCGC(-3/-1)
AfaI 542 GTAC GT^AC
AseI 1097 ATTAAT AT^TAAT
AsnI 1097 ATTAAT AT^TAAT
Asp700I 1953 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 346 CTCGAG C^[CT]CG[GA]G
BanI 1331 GGCACC G^G[CT][GA]CC
BclI 1167 TGATCA T^GATCA
BglII 163 AGATCT A^GATCT
BlpI 1293 GCTAAGC GC^T[ACGT]AGC
BpmI 609 CTCCAG CTGGAG(16/14)
Bpu1102I 1293 GCTAAGC GC^T[ACGT]AGC
BsaI 2085 GAGACC GGTCTC(1/5)
BsmI 87 GCATTC GAATGC(1/-1)
BsoBI 346 CTCGAG C^[CT]CG[GA]G
BspMI 1930 GCAGGT ACCTGC(4/8)
Bst1107I 2114 GTATAC GTA^TAC
BstAPI 1380 GCAGTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1069 TTCGAA TT^CGAA
BstYI 163 AGATCT [GA]^GATC[CT]
BstZ17I 2114 GTATAC GTA^TAC
CelII 1293 GCTAAGC GC^T[ACGT]AGC
CfoI 207 GCGC GCG^C
EcoRV 1807 GATATC GAT^ATC
EcoT22I 1387 ATGCAT ATGCA^T
FauI 134 GCGGG CCCGC(4/6)
FbaI 1167 TGATCA T^GATCA
HhaI 207 GCGC GCG^C
HinP1I 207 GCGC G^CGC
HincII 1834 GTTAAC GT[CT]^[GA]AC
HindII 1834 GTTAAC GT[CT]^[GA]AC
HindIII 1592 AAGCTT A^AGCTT
HpaI 1834 GTTAAC GTT^AAC
MflI 163 AGATCT [GA]^GATC[CT]
NcoI 519 CCATGG C^CATGG
NsiI 1387 ATGCAT ATGCA^T
NspV 1069 TTCGAA TT^CGAA
PaeR7I 346 CTCGAG C^TCGAG
PleI 1232 GACTC GAGTC(4/5)
PshBI 1097 ATTAAT AT^TAAT
RsaI 542 GTAC GT^AC
SacI 197 GAGCTC GAGCT^C
SfuI 1069 TTCGAA TT^CGAA
SmlI 346 CTCGAG C^T[CT][GA]AG
XhoI 346 CTCGAG C^TCGAG
XhoII 163 AGATCT [GA]^GATC[CT]
XmnI 1953 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 1489 GTGCAC G^TGCAC
1712 GTGCAC
AlwI 442 GGATC GGATC(4/5)
1256 GGATC
AocI 406 CCTCAGG CC^T[ACGT]AGG
1363 CCTTAGG
ApaLI 1489 GTGCAC G^TGCAC
1712 GTGCAC
BanII 72 GAGCCC G[GA]GC[CT]^C
197 GAGCTC
BcnI 114 CCGGG CC^[GC]GG
260 CCCGG
BseRI 734 CTCCTC GAGGAG(10/8)
481 GAGGAG
BsmFI 1140 GGGAC GGGAC(10/14)
1920 GTCCC
BspHI 722 TCATGA T^CATGA
1151 TCATGA
Bsu36I 406 CCTCAGG CC^T[ACGT]AGG
1363 CCTTAGG
DraII 402 GGGTCCT [GA]G^G[ACGT]CC[CT]
790 AGGACCT
DsaI 100 CCACGG C^C[GA][CT]GG
519 CCATGG
Eco81I 406 CCTCAGG CC^T[ACGT]AGG
1363 CCTTAGG
EcoO109I 402 GGGTCCT [GA]G^G[ACGT]CC[CT]
790 AGGACCT
HapII 114 CCGG C^CGG
261 CCGG
HpaII 114 CCGG C^CGG
261 CCGG
MspI 114 CCGG C^CGG
261 CCGG
NciI 114 CCGGG CC^[GC]GG
260 CCCGG
PpuMI 402 GGGTCCT [GA]G^G[AT]CC[CT]
790 AGGACCT
PstI 82 CTGCAG CTGCA^G
397 CTGCAG
PvuII 394 CAGCTG CAG^CTG
1337 CAGCTG
RcaI 722 TCATGA T^CATGA
1151 TCATGA
StuI 1411 AGGCCT AGG^CCT
2075 AGGCCT
Tsp45I 557 GTGAC ^GT[GC]AC
596 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccI AcsI AluI ApoI AspHI
AvaII BbvI BfaI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I