##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AhdI AlwNI AocI ApaI
AscI AseI AsnI AspEI AviII
AvrII BanII BbrPI BcgI BciVI
BfrI BglII BlnI BsaAI BsaBI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FseI FspI HpaI
KspI MamI MfeI MluI MroI
MunI NaeI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SfuI SgrAI SmlI SnaBI
SpeI SphI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
AatII 217 GACGTC GACGT^C
Acc65I 1446 GGTACC G^GTACC
AccII 1160 CGCG CG^CG
Aor51HI 798 AGCGCT AGC^GCT
Asp700I 974 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1446 GGTACC G^GTACC
AspI 1359 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 842 CCCGGG C^[CT]CG[GA]G
BalI 1007 TGGCCA TGG^CCA
BbeI 172 GGCGCC GGCGC^C
BclI 647 TGATCA T^GATCA
BglI 40 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 1663 CTGCAC GTGCAG(16/14)
BsmI 1581 GCATTC GAATGC(1/-1)
BsoBI 842 CCCGGG C^[CT]CG[GA]G
BspHI 434 TCATGA T^CATGA
BspMI 1489 GCAGGT ACCTGC(4/8)
BsrBI 107 CCGCTC CCGCTC(-3/-3)
BssSI 836 CTCGTG CACGAG(-5/-1)
BstUI 1160 CGCG CG^CG
BstXI 1010 CCACTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 827 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1576 GAAGAG CTCTTC(1/4)
Eco47III 798 AGCGCT AGC^GCT
EcoRV 1309 GATATC GAT^ATC
FauI 102 GCGGG CCCGC(4/6)
FbaI 647 TGATCA T^GATCA
HgaI 73 GCGTC GACGC(5/10)
HincII 1356 GTTGAC GT[CT]^[GA]AC
HindII 1356 GTTGAC GT[CT]^[GA]AC
HindIII 1641 AAGCTT A^AGCTT
KasI 172 GGCGCC G^GCGCC
KpnI 1446 GGTACC GGTAC^C
Ksp632I 1576 GAAGAG CTCTTC(1/4)
MluNI 1007 TGGCCA TGG^CCA
MscI 1007 TGGCCA TGG^CCA
MvnI 1160 CGCG CG^CG
NarI 172 GGCGCC GG^CGCC
NcoI 681 CCATGG C^CATGG
NdeI 1432 CATATG CA^TATG
NheI 579 GCTAGC G^CTAGC
PflFI 1359 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 303 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 114 GACGGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1285 CTGCAG CTGCA^G
PvuII 1190 CAGCTG CAG^CTG
RcaI 434 TCATGA T^CATGA
SapI 1576 GAAGAGC GCTCTTC(1/4)
SbfI 1284 CCTGCAGG CCTGCA^GG
ScaI 375 AGTACT AGT^ACT
SexAI 828 ACCTGGT A^CC[AT]GGT
SfcI 1285 CTGCAG C^T[GA][CT]AG
SfiI 39 GGCCTCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 172 GGCGCC GGC^GCC
SmaI 842 CCCGGG CCC^GGG
Sse8387I 1284 CCTGCAGG CCTGCA^GG
StuI 1517 AGGCCT AGG^CCT
TaqI 572 TCGA T^CGA
Tsp45I 553 GTGAC ^GT[GC]AC
Tth111I 1359 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 572 TCGA T^CGA
Van91I 303 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1203 TCTAGA T^CTAGA
XmaCI 842 CCCGGG C^CCGGG
XmaI 842 CCCGGG C^CCGGG
XmnI 974 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 401 AAATTC [GA]^AATT[CT]
545 AAATTC
AcyI 172 GGCGCC G[GA]^CG[CT]C
217 GACGTC
Alw44I 389 GTGCAC G^TGCAC
1370 GTGCAC
ApaLI 389 GTGCAC G^TGCAC
1370 GTGCAC
ApoI 401 AAATTC [GA]^AATT[CT]
545 AAATTC
BamHI 1197 GGATCC G^GATCC
1227 GGATCC
BanI 172 GGCGCC G^G[CT][GA]CC
1446 GGTACC
BbsI 1348 GAAGAC GAAGAC(2/6)
1420 GTCTTC
BbvCI 505 GCTGAGG CCTCAGC(-5/-2)
1414 GCTGAGG
BfaI 580 CTAG C^TAG
1204 CTAG
BlpI 88 GCTCAGC GC^T[ACGT]AGC
1171 GCTGAGC
Bpu1102I 88 GCTCAGC GC^T[ACGT]AGC
1171 GCTGAGC
BpuAI 1348 GAAGAC GAAGAC(2/6)
1420 GTCTTC
BsaHI 172 GGCGCC G[GA]^CG[CT]C
217 GACGTC
Bsp1407I 1071 TGTACA T^GTACA
1726 TGTACA
BsrGI 1071 TGTACA T^GTACA
1726 TGTACA
CelII 88 GCTCAGC GC^T[ACGT]AGC
1171 GCTGAGC
DsaI 681 CCATGG C^C[GA][CT]GG
1266 CCGTGG
EaeI 26 TGGCCG [CT]^GGCC[GA]
1007 TGGCCA
EcoT14I 593 CCAAGG C^C[AT][AT]GG
681 CCATGG
HaeII 172 GGCGCC [GA]GCGC^[CT]
798 AGCGCT
Hin1I 172 GGCGCC G[GA]^CG[CT]C
217 GACGTC
MaeI 580 CTAG C^TAG
1204 CTAG
MaeII 218 ACGT A^CGT
1126 ACGT
MaeIII 553 GTGAC ^GT[ACGT]AC
689 GTTAC
MspA1I 29 CCGCTG C[AC]G^C[GT]G
1190 CAGCTG
PleI 59 GACTC GAGTC(4/5)
1207 GACTC
SspBI 1071 TGTACA T^GTACA
1726 TGTACA
StyI 593 CCAAGG C^C[AT][AT]GG
681 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BcnI BmyI BpmI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp509I
TspRI XcmI XhoII