##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 123 GTCTAC GT^[AC][GT]AC
AflIII 1804 ACATGT A^C[GA][CT]GT
Alw44I 1524 GTGCAC G^TGCAC
ApaLI 1524 GTGCAC G^TGCAC
Asp700I 340 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1834 GGATCC G^GATCC
BglI 85 GCCGTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 32 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 32 GCTGAGC GC^T[ACGT]AGC
BsmI 323 GAATGC GAATGC(1/-1)
BspLU11I 1804 ACATGT A^CATGT
BsrDI 1383 CATTGC GCAATG(2/0)
BstYI 1834 GGATCC [GA]^GATC[CT]
CelII 32 GCTGAGC GC^T[ACGT]AGC
EarI 1425 GAAGAG CTCTTC(1/4)
HindIII 343 AAGCTT A^AGCTT
Ksp632I 1425 GAAGAG CTCTTC(1/4)
MflI 1834 GGATCC [GA]^GATC[CT]
PflMI 589 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1313 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1401 AGTACT AGT^ACT
SfaNI 1220 GATGC GCATC(5/9)
SphI 1032 GCATGC GCATG^C
SspI 731 AATATT AAT^ATT
TaqI 501 TCGA T^CGA
TthHB8I 501 TCGA T^CGA
Van91I 589 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1834 GGATCC [GA]^GATC[CT]
XmnI 340 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 1411 AAATTC [GA]^AATT[CT]
1599 AAATTC
AlwI 1835 GATCC GGATC(4/5)
1834 GGATC
AocI 128 CCTGAGG CC^T[ACGT]AGG
1416 CCTAAGG
ApoI 1411 AAATTC [GA]^AATT[CT]
1599 AAATTC
AspI 701 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1334 GACTGGGTC
BanII 35 GAGCCC G[GA]GC[CT]^C
471 GAGCCC
BfaI 1122 CTAG C^TAG
1576 CTAG
BsaI 661 GGTCTC GGTCTC(1/5)
1319 GGTCTC
BsrFI 578 GCCGGC [GA]^CCGG[CT]
1136 GCCGGC
Bsu36I 128 CCTGAGG CC^T[ACGT]AGG
1416 CCTAAGG
Cfr10I 578 GCCGGC [GA]^CCGG[CT]
1136 GCCGGC
DpnII 1835 GATC ^GATC
1881 GATC
Eco81I 128 CCTGAGG CC^T[ACGT]AGG
1416 CCTAAGG
EcoT14I 507 CCAAGG C^C[AT][AT]GG
1343 CCTTGG
HaeII 787 GGCGCT [GA]GCGC^[CT]
909 AGCGCC
HgaI 1554 GCGTC GACGC(5/10)
1782 GCGTC
MaeI 1122 CTAG C^TAG
1576 CTAG
MaeII 738 ACGT A^CGT
1020 ACGT
MboI 1835 GATC ^GATC
1881 GATC
NaeI 578 GCCGGC GCC^GGC
1136 GCCGGC
NdeII 1835 GATC ^GATC
1881 GATC
NgoMIV 578 GCCGGC G^CCGGC
1136 GCCGGC
PflFI 701 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1334 GACTGGGTC
Sau3AI 1835 GATC ^GATC
1881 GATC
SbfI 651 CCTGCAGG CCTGCA^GG
1823 CCTGCAGG
Sse8387I 651 CCTGCAGG CCTGCA^GG
1823 CCTGCAGG
StuI 1182 AGGCCT AGG^CCT
1375 AGGCCT
StyI 507 CCAAGG C^C[AT][AT]GG
1343 CCTTGG
TfiI 403 GAATC G^A[AT]TC
1257 GATTC
Tth111I 701 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1334 GACTGGGTC
XcmI 46 CCACACGAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
894 CCATCGCCACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BalI BanI BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DraII DraIII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau96I ScrFI
SfcI SmaI Tru9I TseI Tsp45I
Tsp509I TspRI XmaCI XmaI