##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccII AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI AspEI AspI AvaI
AviII AvrII BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BlnI
BsaAI BsaBI BsaHI BsiWI BsmI
BsoBI Bsp1407I BspDI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 803 GGTACC G^GTACC
AccI 1297 GTCTAC GT^[AC][GT]AC
AccIII 697 TCCGGA T^CCGGA
AclI 975 AACGTT AA^CGTT
Asp700I 1109 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 803 GGTACC G^GTACC
AspHI 417 GAGCTC G[AT]GC[AT]^C
BamHI 68 GGATCC G^GATCC
BanI 803 GGTACC G^G[CT][GA]CC
BclI 631 TGATCA T^GATCA
BglII 105 AGATCT A^GATCT
BlpI 1376 GCTCAGC GC^T[ACGT]AGC
BpmI 79 CTCCAG CTGGAG(16/14)
Bpu1102I 1376 GCTCAGC GC^T[ACGT]AGC
BseAI 697 TCCGGA T^CCGGA
BsgI 725 CTGCAC GTGCAG(16/14)
BsiEI 563 CGACCG CG[GA][CT]^CG
BsiHKAI 417 GAGCTC G[AT]GC[AT]^C
BsmBI 986 CGTCTC CGTCTC(1/5)
BsmFI 1 GGGAC GGGAC(10/14)
BspEI 697 TCCGGA T^CCGGA
BspMI 452 GCAGGT ACCTGC(4/8)
BstAPI 535 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 908 GGTCACC G^GT[ACGT]ACC
BstPI 908 GGTCACC G^GT[ACGT]ACC
CelII 1376 GCTCAGC GC^T[ACGT]AGC
DraII 1221 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 880 CCTCTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1221 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 908 GGTCACC G^GT[ACGT]ACC
FbaI 631 TGATCA T^GATCA
HincII 227 GTCAAC GT[CT]^[GA]AC
HindII 227 GTCAAC GT[CT]^[GA]AC
KpnI 803 GGTACC GGTAC^C
MaeII 976 ACGT A^CGT
MroI 697 TCCGGA T^CCGGA
NheI 30 GCTAGC G^CTAGC
NspI 1024 GCATGT [GA]CATG^[CT]
PpuMI 1221 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 975 AACGTT AA^CGTT
SacI 417 GAGCTC GAGCT^C
TaqI 562 TCGA T^CGA
Tsp509I 1391 AATT ^AATT
TthHB8I 562 TCGA T^CGA
XbaI 1360 TCTAGA T^CTAGA
XmnI 1109 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 69 GATCC GGATC(4/5)
68 GGATC
AlwNI 5 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1250 CAGTATCTG
BbsI 371 GAAGAC GAAGAC(2/6)
939 GTCTTC
BpuAI 371 GAAGAC GAAGAC(2/6)
939 GTCTTC
BsaWI 24 ACCGGA [AT]^CCGG[AT]
697 TCCGGA
BsrBI 1145 GAGCGG CCGCTC(-3/-3)
1164 GAGCGG
BsrDI 274 GCAATG GCAATG(2/0)
1305 GCAATG
BstXI 148 CCATGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
925 CCATCGTGGTGG
BstYI 68 GGATCC [GA]^GATC[CT]
105 AGATCT
EarI 420 CTCTTC CTCTTC(1/4)
1109 GAAGAG
Ksp632I 420 CTCTTC CTCTTC(1/4)
1109 GAAGAG
MflI 68 GGATCC [GA]^GATC[CT]
105 AGATCT
MseI 91 TTAA T^TAA
1018 TTAA
PflMI 168 CCACTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
730 CCATCAACTGG
PleI 652 GAGTC GAGTC(4/5)
1385 GAGTC
PvuII 722 CAGCTG CAG^CTG
1061 CAGCTG
SapI 1109 GAAGAGC GCTCTTC(1/4)
419 GCTCTTC
TfiI 747 GAATC G^A[AT]TC
865 GAATC
Tru9I 91 TTAA T^TAA
1018 TTAA
Van91I 168 CCACTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
730 CCATCAACTGG
XcmI 467 CCACTGGCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
487 CCATTTGCCGGGTGG
XhoII 68 GGATCC [GA]^GATC[CT]
105 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AvaII BalI
BanII BbvI BcnI BfaI BmyI
BsaI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI