##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NdeI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AfaI 1655 GTAC GT^AC
AocI 517 CCTGAGG CC^T[ACGT]AGG
BalI 453 TGGCCA TGG^CCA
BamHI 274 GGATCC G^GATCC
BbsI 1010 GTCTTC GAAGAC(2/6)
BbvCI 1363 GCTGAGG CCTCAGC(-5/-2)
BpuAI 1010 GTCTTC GAAGAC(2/6)
BsiEI 50 CGGCCG CG[GA][CT]^CG
BstAPI 1252 GCAGACGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 517 CCTGAGG CC^T[ACGT]AGG
EagI 50 CGGCCG C^GGCCG
EclXI 50 CGGCCG C^GGCCG
Eco52I 50 CGGCCG C^GGCCG
Eco81I 517 CCTGAGG CC^T[ACGT]AGG
HindIII 1523 AAGCTT A^AGCTT
MluNI 453 TGGCCA TGG^CCA
MscI 453 TGGCCA TGG^CCA
NaeI 182 GCCGGC GCC^GGC
NcoI 81 CCATGG C^CATGG
NgoMIV 182 GCCGGC G^CCGGC
NotI 49 GCGGCCGC GC^GGCCGC
NspI 1317 ACATGC [GA]CATG^[CT]
RsaI 1655 GTAC GT^AC
SacI 134 GAGCTC GAGCT^C
SbfI 373 CCTGCAGG CCTGCA^GG
SexAI 1657 ACCAGGT A^CC[AT]GGT
SfaNI 802 GCATC GCATC(5/9)
SfiI 1099 GGCCCGACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 849 CCCGGG CCC^GGG
Sse8387I 373 CCTGCAGG CCTGCA^GG
TfiI 1756 GAATC G^A[AT]TC
XbaI 1584 TCTAGA T^CTAGA
XcmI 78 CCACCATGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 849 CCCGGG C^CCGGG
XmaI 849 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbeI 164 GGCGCC GGCGC^C
700 GGCGCC
BsaI 896 GGTCTC GGTCTC(1/5)
1302 GGTCTC
BsrFI 182 GCCGGC [GA]^CCGG[CT]
625 GCCGGT
BstEII 974 GGTGACC G^GT[ACGT]ACC
1156 GGTCACC
BstPI 974 GGTGACC G^GT[ACGT]ACC
1156 GGTCACC
BstXI 81 CCATGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
444 CCAGCCTGGTGG
BstYI 274 GGATCC [GA]^GATC[CT]
999 AGATCC
Cfr10I 182 GCCGGC [GA]^CCGG[CT]
625 GCCGGT
DsaI 81 CCATGG C^C[GA][CT]GG
1206 CCGTGG
EarI 137 CTCTTC CTCTTC(1/4)
1334 GAAGAG
EcoO65I 974 GGTGACC G^GT[ACGT]ACC
1156 GGTCACC
KasI 164 GGCGCC G^GCGCC
700 GGCGCC
Ksp632I 137 CTCTTC CTCTTC(1/4)
1334 GAAGAG
MflI 274 GGATCC [GA]^GATC[CT]
999 AGATCC
MslI 1147 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1312 CATGGACATG
NarI 164 GGCGCC GG^CGCC
700 GGCGCC
NheI 601 GCTAGC G^CTAGC
1514 GCTAGC
PflMI 704 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1346 CCACAGGCTGG
SapI 1334 GAAGAGC GCTCTTC(1/4)
136 GCTCTTC
SfoI 164 GGCGCC GGC^GCC
700 GGCGCC
Van91I 704 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1346 CCACAGGCTGG
XhoII 274 GGATCC [GA]^GATC[CT]
999 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssHII BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII Sau3AI Sau96I ScrFI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I