##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbeI BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HindIII KasI KspI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1359 GGTACC G^GTACC
AccII 592 CGCG CG^CG
AcsI 1407 GAATTC [GA]^AATT[CT]
AcyI 341 GGCGTC G[GA]^CG[CT]C
AfaI 1360 GTAC GT^AC
Alw44I 273 GTGCAC G^TGCAC
ApaLI 273 GTGCAC G^TGCAC
ApoI 1407 GAATTC [GA]^AATT[CT]
Asp700I 1436 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1359 GGTACC G^GTACC
AvaI 484 CCCGGG C^[CT]CG[GA]G
BalI 459 TGGCCA TGG^CCA
BbvCI 8 CCTCAGC CCTCAGC(-5/-2)
BlpI 1179 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1179 GCTGAGC GC^T[ACGT]AGC
BsaHI 341 GGCGTC G[GA]^CG[CT]C
BseRI 289 CTCCTC GAGGAG(10/8)
BsoBI 484 CCCGGG C^[CT]CG[GA]G
BsrFI 170 GCCGGC [GA]^CCGG[CT]
BssSI 995 CTCGTG CACGAG(-5/-1)
BstUI 592 CGCG CG^CG
CelII 1179 GCTGAGC GC^T[ACGT]AGC
Cfr10I 170 GCCGGC [GA]^CCGG[CT]
DrdI 662 GACGTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 678 CCATGG C^C[GA][CT]GG
EarI 20 CTCTTC CTCTTC(1/4)
EcoRI 1407 GAATTC G^AATTC
Hin1I 341 GGCGTC G[GA]^CG[CT]C
HpaI 1205 GTTAAC GTT^AAC
KpnI 1359 GGTACC GGTAC^C
Ksp632I 20 CTCTTC CTCTTC(1/4)
MluNI 459 TGGCCA TGG^CCA
MscI 459 TGGCCA TGG^CCA
MseI 1206 TTAA T^TAA
MvnI 592 CGCG CG^CG
NaeI 170 GCCGGC GCC^GGC
NcoI 678 CCATGG C^CATGG
NdeI 321 CATATG CA^TATG
NgoMIV 170 GCCGGC G^CCGGC
PleI 71 GACTC GAGTC(4/5)
PstI 641 CTGCAG CTGCA^G
RsaI 1360 GTAC GT^AC
SexAI 1355 ACCAGGT A^CC[AT]GGT
SmaI 484 CCCGGG CCC^GGG
TaqI 453 TCGA T^CGA
Tru9I 1206 TTAA T^TAA
TthHB8I 453 TCGA T^CGA
XmaCI 484 CCCGGG C^CCGGG
XmaI 484 CCCGGG C^CCGGG
XmnI 1436 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 182 GTCTAC GT^[AC][GT]AC
494 GTCTAC
AlwNI 635 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
794 CAGGCCCTG
BbrPI 125 CACGTG CAC^GTG
941 CACGTG
BbsI 130 GAAGAC GAAGAC(2/6)
778 GAAGAC
BfaI 470 CTAG C^TAG
1120 CTAG
BglI 908 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1255 GCCAGCGAGGC
BpuAI 130 GAAGAC GAAGAC(2/6)
778 GAAGAC
BsaAI 125 CACGTG [CT]AC^GT[GA]
941 CACGTG
BsaI 1129 GAGACC GGTCTC(1/5)
1384 GAGACC
BsgI 122 CTGCAC GTGCAG(16/14)
1199 GTGCAG
BsmFI 660 GGGAC GGGAC(10/14)
344 GTCCC
BspMI 376 ACCTGC ACCTGC(4/8)
1201 GCAGGT
BstYI 225 AGATCC [GA]^GATC[CT]
891 AGATCC
HgaI 180 GCGTC GACGC(5/10)
342 GCGTC
HincII 1205 GTTAAC GT[CT]^[GA]AC
1455 GTCAAC
HindII 1205 GTTAAC GT[CT]^[GA]AC
1455 GTCAAC
MaeI 470 CTAG C^TAG
1120 CTAG
MflI 225 AGATCC [GA]^GATC[CT]
891 AGATCC
PmaCI 125 CACGTG CAC^GTG
941 CACGTG
PmlI 125 CACGTG CAC^GTG
941 CACGTG
PvuII 1037 CAGCTG CAG^CTG
1058 CAGCTG
SfcI 641 CTGCAG C^T[GA][CT]AG
934 CTATAG
TfiI 970 GAATC G^A[AT]TC
1391 GAATC
XcmI 414 CCACAGTCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1125 CCAAGAGACCCCTGG
XhoII 225 AGATCC [GA]^GATC[CT]
891 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SmlI StyI TseI Tsp45I
Tsp509I TspRI