################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI Asp718I AspEI AspI AviII AvrII BalI BamHI BbsI BbvCI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DrdI Eam1105I Eco47III EcoO65I EcoRV EcoT22I FseI FspI KpnI MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NruI NsiI PacI PflFI PinAI PmeI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### ApaI 143 GGGCCC GGGCC^C AseI 2189 ATTAAT AT^TAAT AsnI 2189 ATTAAT AT^TAAT Asp700I 1318 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 184 GAGCTC G[AT]GC[AT]^C BbrPI 974 CACGTG CAC^GTG BcgI 2090 GCAGTTCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 688 GGATAC GTATCC(6/5) BclI 1368 TGATCA T^GATCA BsiHKAI 184 GAGCTC G[AT]GC[AT]^C BsmBI 411 CGTCTC CGTCTC(1/5) BsrFI 382 GCCGGC [GA]^CCGG[CT] BstBI 2098 TTCGAA TT^CGAA BstYI 594 AGATCC [GA]^GATC[CT] Cfr10I 382 GCCGGC [GA]^CCGG[CT] DraI 2276 TTTAAA TTT^AAA EcoNI 1749 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2248 GAATTC G^AATTC FbaI 1368 TGATCA T^GATCA HindIII 1079 AAGCTT A^AGCTT HpaI 1606 GTTAAC GTT^AAC MflI 594 AGATCC [GA]^GATC[CT] MslI 1714 CATTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 382 GCCGGC GCC^GGC NgoMIV 382 GCCGGC G^CCGGC NotI 386 GCGGCCGC GC^GGCCGC NspV 2098 TTCGAA TT^CGAA PaeR7I 1520 CTCGAG C^TCGAG PflMI 1678 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 974 CACGTG CAC^GTG PmlI 974 CACGTG CAC^GTG PpuMI 1533 AGGACCC [GA]G^G[AT]CC[CT] PshBI 2189 ATTAAT AT^TAAT SacI 184 GAGCTC GAGCT^C SapI 1043 GCTCTTC GCTCTTC(1/4) SexAI 3 ACCAGGT A^CC[AT]GGT SfuI 2098 TTCGAA TT^CGAA Van91I 1678 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1520 CTCGAG C^TCGAG XhoII 594 AGATCC [GA]^GATC[CT] XmnI 1318 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccI 1229 GTAGAC GT^[AC][GT]AC 1688 GTCTAC AcsI 2063 AAATTT [GA]^AATT[CT] 2248 GAATTC ApoI 2063 AAATTT [GA]^AATT[CT] 2248 GAATTC AvaII 64 GGACC G^G[AT]CC 1534 GGACC BanI 158 GGCGCC G^G[CT][GA]CC 311 GGCGCC BbeI 158 GGCGCC GGCGC^C 311 GGCGCC BpmI 346 CTCCAG CTGGAG(16/14) 710 CTGGAG BsaAI 974 CACGTG [CT]AC^GT[GA] 1544 TACGTG BsaI 266 GAGACC GGTCTC(1/5) 713 GAGACC BsiEI 108 CGGCCG CG[GA][CT]^CG 387 CGGCCG Bsp1407I 1722 TGTACA T^GTACA 1863 TGTACA BspMI 1890 ACCTGC ACCTGC(4/8) 1427 GCAGGT BsrBI 343 CCGCTC CCGCTC(-3/-3) 131 GAGCGG BsrDI 1094 GCAATG GCAATG(2/0) 2182 GCAATG BsrGI 1722 TGTACA T^GTACA 1863 TGTACA BstF5I 913 CATCC GGATG(2/0) 1966 GGATG DraIII 2 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 2136 CACTCGGTG EagI 108 CGGCCG C^GGCCG 387 CGGCCG EclXI 108 CGGCCG C^GGCCG 387 CGGCCG Eco52I 108 CGGCCG C^GGCCG 387 CGGCCG EcoT14I 138 CCAAGG C^C[AT][AT]GG 1630 CCAAGG FokI 913 CATCC GGATG(9/13) 1966 GGATG HincII 1034 GTCAAC GT[CT]^[GA]AC 1606 GTTAAC HindII 1034 GTCAAC GT[CT]^[GA]AC 1606 GTTAAC KasI 158 GGCGCC G^GCGCC 311 GGCGCC KspI 105 CCGCGG CCGC^GG 396 CCGCGG NarI 158 GGCGCC GG^CGCC 311 GGCGCC NspI 1515 GCATGC [GA]CATG^[CT] 1650 GCATGC PleI 1148 GACTC GAGTC(4/5) 1941 GACTC PshAI 65 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1580 GACATAGGTC PstI 650 CTGCAG CTGCA^G 1283 CTGCAG PvuII 421 CAGCTG CAG^CTG 1286 CAGCTG SacII 105 CCGCGG CCGC^GG 396 CCGCGG SfoI 158 GGCGCC GGC^GCC 311 GGCGCC SmlI 1073 CTTGAG C^T[CT][GA]AG 1520 CTCGAG SphI 1515 GCATGC GCATG^C 1650 GCATGC SspBI 1722 TGTACA T^GTACA 1863 TGTACA StyI 138 CCAAGG C^C[AT][AT]GG 1630 CCAAGG TfiI 931 GATTC G^A[AT]TC 1134 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AocI AvaI BanII BbvI BcnI BfaI BglI BmyI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstAPI BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmaCI XmaI