##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbsI BbvCI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BssHII BssSI Bst1107I
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DrdI Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FseI FspI
KpnI MamI MfeI MluI MluNI
MroI MscI MunI NcoI NdeI
NheI NruI NsiI PacI PflFI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I XbaI XcmI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
ApaI 143 GGGCCC GGGCC^C
AseI 2189 ATTAAT AT^TAAT
AsnI 2189 ATTAAT AT^TAAT
Asp700I 1318 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 184 GAGCTC G[AT]GC[AT]^C
BbrPI 974 CACGTG CAC^GTG
BcgI 2090 GCAGTTCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 688 GGATAC GTATCC(6/5)
BclI 1368 TGATCA T^GATCA
BsiHKAI 184 GAGCTC G[AT]GC[AT]^C
BsmBI 411 CGTCTC CGTCTC(1/5)
BsrFI 382 GCCGGC [GA]^CCGG[CT]
BstBI 2098 TTCGAA TT^CGAA
BstYI 594 AGATCC [GA]^GATC[CT]
Cfr10I 382 GCCGGC [GA]^CCGG[CT]
DraI 2276 TTTAAA TTT^AAA
EcoNI 1749 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2248 GAATTC G^AATTC
FbaI 1368 TGATCA T^GATCA
HindIII 1079 AAGCTT A^AGCTT
HpaI 1606 GTTAAC GTT^AAC
MflI 594 AGATCC [GA]^GATC[CT]
MslI 1714 CATTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 382 GCCGGC GCC^GGC
NgoMIV 382 GCCGGC G^CCGGC
NotI 386 GCGGCCGC GC^GGCCGC
NspV 2098 TTCGAA TT^CGAA
PaeR7I 1520 CTCGAG C^TCGAG
PflMI 1678 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 974 CACGTG CAC^GTG
PmlI 974 CACGTG CAC^GTG
PpuMI 1533 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 2189 ATTAAT AT^TAAT
SacI 184 GAGCTC GAGCT^C
SapI 1043 GCTCTTC GCTCTTC(1/4)
SexAI 3 ACCAGGT A^CC[AT]GGT
SfuI 2098 TTCGAA TT^CGAA
Van91I 1678 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1520 CTCGAG C^TCGAG
XhoII 594 AGATCC [GA]^GATC[CT]
XmnI 1318 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1229 GTAGAC GT^[AC][GT]AC
1688 GTCTAC
AcsI 2063 AAATTT [GA]^AATT[CT]
2248 GAATTC
ApoI 2063 AAATTT [GA]^AATT[CT]
2248 GAATTC
AvaII 64 GGACC G^G[AT]CC
1534 GGACC
BanI 158 GGCGCC G^G[CT][GA]CC
311 GGCGCC
BbeI 158 GGCGCC GGCGC^C
311 GGCGCC
BpmI 346 CTCCAG CTGGAG(16/14)
710 CTGGAG
BsaAI 974 CACGTG [CT]AC^GT[GA]
1544 TACGTG
BsaI 266 GAGACC GGTCTC(1/5)
713 GAGACC
BsiEI 108 CGGCCG CG[GA][CT]^CG
387 CGGCCG
Bsp1407I 1722 TGTACA T^GTACA
1863 TGTACA
BspMI 1890 ACCTGC ACCTGC(4/8)
1427 GCAGGT
BsrBI 343 CCGCTC CCGCTC(-3/-3)
131 GAGCGG
BsrDI 1094 GCAATG GCAATG(2/0)
2182 GCAATG
BsrGI 1722 TGTACA T^GTACA
1863 TGTACA
BstF5I 913 CATCC GGATG(2/0)
1966 GGATG
DraIII 2 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2136 CACTCGGTG
EagI 108 CGGCCG C^GGCCG
387 CGGCCG
EclXI 108 CGGCCG C^GGCCG
387 CGGCCG
Eco52I 108 CGGCCG C^GGCCG
387 CGGCCG
EcoT14I 138 CCAAGG C^C[AT][AT]GG
1630 CCAAGG
FokI 913 CATCC GGATG(9/13)
1966 GGATG
HincII 1034 GTCAAC GT[CT]^[GA]AC
1606 GTTAAC
HindII 1034 GTCAAC GT[CT]^[GA]AC
1606 GTTAAC
KasI 158 GGCGCC G^GCGCC
311 GGCGCC
KspI 105 CCGCGG CCGC^GG
396 CCGCGG
NarI 158 GGCGCC GG^CGCC
311 GGCGCC
NspI 1515 GCATGC [GA]CATG^[CT]
1650 GCATGC
PleI 1148 GACTC GAGTC(4/5)
1941 GACTC
PshAI 65 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1580 GACATAGGTC
PstI 650 CTGCAG CTGCA^G
1283 CTGCAG
PvuII 421 CAGCTG CAG^CTG
1286 CAGCTG
SacII 105 CCGCGG CCGC^GG
396 CCGCGG
SfoI 158 GGCGCC GGC^GCC
311 GGCGCC
SmlI 1073 CTTGAG C^T[CT][GA]AG
1520 CTCGAG
SphI 1515 GCATGC GCATG^C
1650 GCATGC
SspBI 1722 TGTACA T^GTACA
1863 TGTACA
StyI 138 CCAAGG C^C[AT][AT]GG
1630 CCAAGG
TfiI 931 GATTC G^A[AT]TC
1134 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI AvaI BanII BbvI
BcnI BfaI BglI BmyI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstAPI BstNI BstUI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmaCI XmaI