##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AvaII AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I Cfr13I
ClaI CpoI DraII DraIII Eam1105I
Eco47III Eco81I EcoO109I EcoO65I EcoRI
EcoRV FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KspI MamI MluI MroI MspA1I
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PaeR7I PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuII RcaI RsrII SacII SalI
Sau96I SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI SwaI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 1000 GGTACC G^GTACC
AlwI 1509 GGATC GGATC(4/5)
Asp700I 1088 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1000 GGTACC G^GTACC
AspI 1208 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 159 CCCGGG C^[CT]CG[GA]G
BalI 1485 TGGCCA TGG^CCA
BanII 913 GAGCTC G[GA]GC[CT]^C
BbrPI 902 CACGTG CAC^GTG
BclI 993 TGATCA T^GATCA
BsaAI 902 CACGTG [CT]AC^GT[GA]
BseRI 796 GAGGAG GAGGAG(10/8)
BsgI 1311 GTGCAG GTGCAG(16/14)
BsmI 698 GCATTC GAATGC(1/-1)
BsoBI 159 CCCGGG C^[CT]CG[GA]G
BspLU11I 1057 ACATGT A^CATGT
BsrDI 1734 GCAATG GCAATG(2/0)
DrdI 379 GACCCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 130 CGGCCG C^GGCCG
EclXI 130 CGGCCG C^GGCCG
Eco52I 130 CGGCCG C^GGCCG
EcoT22I 594 ATGCAT ATGCA^T
FauI 986 CCCGC CCCGC(4/6)
FbaI 993 TGATCA T^GATCA
HapII 160 CCGG C^CGG
HindIII 229 AAGCTT A^AGCTT
HpaII 160 CCGG C^CGG
KpnI 1000 GGTACC GGTAC^C
MaeII 903 ACGT A^CGT
MfeI 1602 CAATTG C^AATTG
MluNI 1485 TGGCCA TGG^CCA
MscI 1485 TGGCCA TGG^CCA
MslI 196 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 160 CCGG C^CGG
MunI 1602 CAATTG C^AATTG
NcoI 1523 CCATGG C^CATGG
NsiI 594 ATGCAT ATGCA^T
PacI 1133 TTAATTAA TTAAT^TAA
PflFI 1208 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 902 CACGTG CAC^GTG
PmlI 902 CACGTG CAC^GTG
PstI 383 CTGCAG CTGCA^G
PvuI 75 CGATCG CGAT^CG
SacI 913 GAGCTC GAGCT^C
SapI 1197 GAAGAGC GCTCTTC(1/4)
ScaI 1638 AGTACT AGT^ACT
SmaI 159 CCCGGG CCC^GGG
SpeI 1661 ACTAGT A^CTAGT
SspI 1425 AATATT AAT^ATT
StuI 764 AGGCCT AGG^CCT
Tsp45I 1344 GTGAC ^GT[GC]AC
Tth111I 1208 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 159 CCCGGG C^CCGGG
XmaI 159 CCCGGG C^CCGGG
XmnI 1088 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 245 GTCTAC GT^[AC][GT]AC
874 GTAGAC
AflII 1045 CTTAAG C^TTAAG
1182 CTTAAG
AflIII 901 ACACGT A^C[GA][CT]GT
1057 ACATGT
BcnI 159 CCCGG CC^[GC]GG
160 CCGGG
BfrI 1045 CTTAAG C^TTAAG
1182 CTTAAG
BsaI 469 GAGACC GGTCTC(1/5)
1743 GAGACC
BsmAI 469 GAGAC GTCTC(1/5)
1743 GAGAC
BsmFI 362 GGGAC GGGAC(10/14)
1724 GTCCC
Cac8I 2 GCAGGC GC[ACGT]^[ACGT]GC
1268 GCGTGC
CfoI 34 GCGC GCG^C
463 GCGC
DsaI 837 CCACGG C^C[GA][CT]GG
1523 CCATGG
EaeI 130 CGGCCG [CT]^GGCC[GA]
1485 TGGCCA
EcoNI 452 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
990 CCTTGATCAGG
HhaI 34 GCGC GCG^C
463 GCGC
HinP1I 34 GCGC G^CGC
463 GCGC
HphI 1168 GGTGA GGTGA(8/7)
117 TCACC
MwoI 915 GCTCAAGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
924 GCAGCAGAGGC
NciI 159 CCCGG CC^[GC]GG
160 CCGGG
NspI 92 ACATGC [GA]CATG^[CT]
1057 ACATGT
PflMI 139 CCACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1488 CCATAAAGTGG
PleI 1346 GACTC GAGTC(4/5)
29 GAGTC
SfcI 383 CTGCAG C^T[GA][CT]AG
1405 CTATAG
TaqI 78 TCGA T^CGA
322 TCGA
TthHB8I 78 TCGA T^CGA
322 TCGA
Van91I 139 CCACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1488 CCATAAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI BanI BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaJI BsiEI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI MvnI NdeII NlaIII
NlaIV RsaI Sau3AI ScrFI SfaNI
SmlI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII