################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccIII AclI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AvaII AviII AvrII BamHI BbeI BbvCI BcgI BciVI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraII DraIII Eam1105I Eco47III Eco81I EcoO109I EcoO65I EcoRI EcoRV FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KspI MamI MluI MroI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuII RcaI RsrII SacII SalI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 1000 GGTACC G^GTACC AlwI 1509 GGATC GGATC(4/5) Asp700I 1088 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1000 GGTACC G^GTACC AspI 1208 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 159 CCCGGG C^[CT]CG[GA]G BalI 1485 TGGCCA TGG^CCA BanII 913 GAGCTC G[GA]GC[CT]^C BbrPI 902 CACGTG CAC^GTG BclI 993 TGATCA T^GATCA BsaAI 902 CACGTG [CT]AC^GT[GA] BseRI 796 GAGGAG GAGGAG(10/8) BsgI 1311 GTGCAG GTGCAG(16/14) BsmI 698 GCATTC GAATGC(1/-1) BsoBI 159 CCCGGG C^[CT]CG[GA]G BspLU11I 1057 ACATGT A^CATGT BsrDI 1734 GCAATG GCAATG(2/0) DrdI 379 GACCCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 130 CGGCCG C^GGCCG EclXI 130 CGGCCG C^GGCCG Eco52I 130 CGGCCG C^GGCCG EcoT22I 594 ATGCAT ATGCA^T FauI 986 CCCGC CCCGC(4/6) FbaI 993 TGATCA T^GATCA HapII 160 CCGG C^CGG HindIII 229 AAGCTT A^AGCTT HpaII 160 CCGG C^CGG KpnI 1000 GGTACC GGTAC^C MaeII 903 ACGT A^CGT MfeI 1602 CAATTG C^AATTG MluNI 1485 TGGCCA TGG^CCA MscI 1485 TGGCCA TGG^CCA MslI 196 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 160 CCGG C^CGG MunI 1602 CAATTG C^AATTG NcoI 1523 CCATGG C^CATGG NsiI 594 ATGCAT ATGCA^T PacI 1133 TTAATTAA TTAAT^TAA PflFI 1208 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 902 CACGTG CAC^GTG PmlI 902 CACGTG CAC^GTG PstI 383 CTGCAG CTGCA^G PvuI 75 CGATCG CGAT^CG SacI 913 GAGCTC GAGCT^C SapI 1197 GAAGAGC GCTCTTC(1/4) ScaI 1638 AGTACT AGT^ACT SmaI 159 CCCGGG CCC^GGG SpeI 1661 ACTAGT A^CTAGT SspI 1425 AATATT AAT^ATT StuI 764 AGGCCT AGG^CCT Tsp45I 1344 GTGAC ^GT[GC]AC Tth111I 1208 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 159 CCCGGG C^CCGGG XmaI 159 CCCGGG C^CCGGG XmnI 1088 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 245 GTCTAC GT^[AC][GT]AC 874 GTAGAC AflII 1045 CTTAAG C^TTAAG 1182 CTTAAG AflIII 901 ACACGT A^C[GA][CT]GT 1057 ACATGT BcnI 159 CCCGG CC^[GC]GG 160 CCGGG BfrI 1045 CTTAAG C^TTAAG 1182 CTTAAG BsaI 469 GAGACC GGTCTC(1/5) 1743 GAGACC BsmAI 469 GAGAC GTCTC(1/5) 1743 GAGAC BsmFI 362 GGGAC GGGAC(10/14) 1724 GTCCC Cac8I 2 GCAGGC GC[ACGT]^[ACGT]GC 1268 GCGTGC CfoI 34 GCGC GCG^C 463 GCGC DsaI 837 CCACGG C^C[GA][CT]GG 1523 CCATGG EaeI 130 CGGCCG [CT]^GGCC[GA] 1485 TGGCCA EcoNI 452 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 990 CCTTGATCAGG HhaI 34 GCGC GCG^C 463 GCGC HinP1I 34 GCGC G^CGC 463 GCGC HphI 1168 GGTGA GGTGA(8/7) 117 TCACC MwoI 915 GCTCAAGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 924 GCAGCAGAGGC NciI 159 CCCGG CC^[GC]GG 160 CCGGG NspI 92 ACATGC [GA]CATG^[CT] 1057 ACATGT PflMI 139 CCACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1488 CCATAAAGTGG PleI 1346 GACTC GAGTC(4/5) 29 GAGTC SfcI 383 CTGCAG C^T[GA][CT]AG 1405 CTATAG TaqI 78 TCGA T^CGA 322 TCGA TthHB8I 78 TCGA T^CGA 322 TCGA Van91I 139 CCACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1488 CCATAAAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI AspHI BanI BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BsiEI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MvnI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI SmlI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII