##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AvrII
BbeI BbrPI BbsI BbvCI BciVI
BfaI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaWI BseAI BsiHKAI
BsiWI BsmBI BsmFI BsmI BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII Eam1105I
Eco47III EcoO65I EcoRV EcoT22I FseI
HaeII HpaI KasI KpnI KspI
MaeI MfeI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SfiI
SfoI SfuI SgrAI SnaBI SpeI
StuI SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 161 GTCTAC GT^[AC][GT]AC
AcyI 1119 GGCGTC G[GA]^CG[CT]C
AlwNI 638 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1016 CCTGAGG CC^T[ACGT]AGG
AseI 1279 ATTAAT AT^TAAT
AsnI 1279 ATTAAT AT^TAAT
AspI 698 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 900 TGCGCA TGC^GCA
BanI 394 GGTGCC G^G[CT][GA]CC
BanII 1034 GAGCCC G[GA]GC[CT]^C
BcgI 772 CGATGTGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 606 TGATCA T^GATCA
BglII 937 AGATCT A^GATCT
BpmI 198 CTGGAG CTGGAG(16/14)
BsaBI 232 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1119 GGCGTC G[GA]^CG[CT]C
BsaI 680 GAGACC GGTCTC(1/5)
BseRI 98 GAGGAG GAGGAG(10/8)
BsiEI 26 CGGCCG CG[GA][CT]^CG
BsmAI 680 GAGAC GTCTC(1/5)
Bsp1407I 1302 TGTACA T^GTACA
BspLU11I 714 ACATGT A^CATGT
BsrGI 1302 TGTACA T^GTACA
Bsu36I 1016 CCTGAGG CC^T[ACGT]AGG
DrdI 152 GACAAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
Eco81I 1016 CCTGAGG CC^T[ACGT]AGG
EcoRI 488 GAATTC G^AATTC
FbaI 606 TGATCA T^GATCA
FspI 900 TGCGCA TGC^GCA
HgaI 1120 GCGTC GACGC(5/10)
Hin1I 1119 GGCGTC G[GA]^CG[CT]C
HincII 704 GTCAAC GT[CT]^[GA]AC
HindII 704 GTCAAC GT[CT]^[GA]AC
HindIII 1422 AAGCTT A^AGCTT
MamI 232 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1051 ACGCGT A^CGCGT
NcoI 54 CCATGG C^CATGG
PflFI 698 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1279 ATTAAT AT^TAAT
SbfI 850 CCTGCAGG CCTGCA^GG
SexAI 390 ACCTGGT A^CC[AT]GGT
SmaI 1059 CCCGGG CCC^GGG
Sse8387I 850 CCTGCAGG CCTGCA^GG
SspBI 1302 TGTACA T^GTACA
SspI 1545 AATATT AAT^ATT
Tth111I 698 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1059 CCCGGG C^CCGGG
XmaI 1059 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 1269 CTTAAG C^TTAAG
1425 CTTAAG
AflIII 714 ACATGT A^C[GA][CT]GT
1051 ACGCGT
Asp700I 424 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
865 GAACCTCTTC
BamHI 237 GGATCC G^GATCC
756 GGATCC
BfrI 1269 CTTAAG C^TTAAG
1425 CTTAAG
BsgI 329 GTGCAG GTGCAG(16/14)
1556 GTGCAG
BstAPI 175 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
934 GCAAGATCTGC
DsaI 54 CCATGG C^C[GA][CT]GG
402 CCGTGG
EarI 869 CTCTTC CTCTTC(1/4)
275 GAAGAG
EcoNI 349 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
850 CCTGCAGGAGG
EcoT14I 54 CCATGG C^C[AT][AT]GG
504 CCAAGG
HinfI 203 GAATC G^A[ACGT]TC
672 GAATC
Ksp632I 869 CTCTTC CTCTTC(1/4)
275 GAAGAG
MspA1I 140 CAGCTG C[AC]G^C[GT]G
563 CAGCTG
PacI 1258 TTAATTAA TTAAT^TAA
1280 TTAATTAA
PstI 143 CTGCAG CTGCA^G
851 CTGCAG
PvuII 140 CAGCTG CAG^CTG
563 CAGCTG
ScaI 582 AGTACT AGT^ACT
1068 AGTACT
SfaNI 1022 GATGC GCATC(5/9)
879 GCATC
SmlI 1269 CTTAAG C^T[CT][GA]AG
1425 CTTAAG
SphI 499 GCATGC GCATG^C
1209 GCATGC
StyI 54 CCATGG C^C[AT][AT]GG
504 CCAAGG
TfiI 203 GAATC G^A[AT]TC
672 GAATC
XmnI 424 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
865 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BalI
BbvI BcnI BmyI BsaJI BsiYI
BslI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PpuMI RsaI
Sau3AI Sau96I ScrFI SfcI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII