##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII Eco81I EcoO65I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MluI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 1572 GTAGAC GT^[AC][GT]AC
AccIII 69 TCCGGA T^CCGGA
AcsI 171 GAATTC [GA]^AATT[CT]
AcyI 1732 GACGCC G[GA]^CG[CT]C
AflIII 1576 ACACGT A^C[GA][CT]GT
Aor51HI 478 AGCGCT AGC^GCT
ApoI 171 GAATTC [GA]^AATT[CT]
AvaI 1652 CCCGAG C^[CT]CG[GA]G
BalI 1745 TGGCCA TGG^CCA
BamHI 1851 GGATCC G^GATCC
BsaHI 1732 GACGCC G[GA]^CG[CT]C
BsaWI 69 TCCGGA [AT]^CCGG[AT]
BseAI 69 TCCGGA T^CCGGA
BsiEI 1102 CGGCCG CG[GA][CT]^CG
BsoBI 1652 CCCGAG C^[CT]CG[GA]G
BspEI 69 TCCGGA T^CCGGA
BsrBI 1544 CCGCTC CCGCTC(-3/-3)
BsrDI 707 CATTGC GCAATG(2/0)
BsrFI 328 ACCGGC [GA]^CCGG[CT]
BssHII 443 GCGCGC G^CGCGC
BstAPI 1609 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 328 ACCGGC [GA]^CCGG[CT]
DraI 734 TTTAAA TTT^AAA
DrdI 1824 GACTGGTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1102 CGGCCG C^GGCCG
EclXI 1102 CGGCCG C^GGCCG
Eco47III 478 AGCGCT AGC^GCT
Eco52I 1102 CGGCCG C^GGCCG
EcoRI 171 GAATTC G^AATTC
EcoRV 809 GATATC GAT^ATC
Hin1I 1732 GACGCC G[GA]^CG[CT]C
MaeIII 1789 GTGAC ^GT[ACGT]AC
MfeI 384 CAATTG C^AATTG
MluNI 1745 TGGCCA TGG^CCA
MroI 69 TCCGGA T^CCGGA
MscI 1745 TGGCCA TGG^CCA
MunI 384 CAATTG C^AATTG
NotI 1101 GCGGCCGC GC^GGCCGC
PflMI 924 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 733 GTTTAAAC GTTT^AAAC
PstI 1199 CTGCAG CTGCA^G
ScaI 1896 AGTACT AGT^ACT
SexAI 1044 ACCAGGT A^CC[AT]GGT
SfcI 1199 CTGCAG C^T[GA][CT]AG
Tsp45I 1789 GTGAC ^GT[GC]AC
Van91I 924 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1842 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 246 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1732 GACGCCACGTC
Asp700I 549 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1151 GAACCTCTTC
AspEI 246 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1732 GACGCCACGTC
AspI 1233 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1309 GACCATGTC
BanI 1458 GGCACC G^G[CT][GA]CC
1814 GGTGCC
BanII 686 GGGCTC G[GA]GC[CT]^C
1867 GAGCCC
BbsI 465 GAAGAC GAAGAC(2/6)
1306 GAAGAC
BcgI 497 CGAAAGAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1387 CGATCTGCCTGC
BfaI 1843 CTAG C^TAG
1894 CTAG
BglII 116 AGATCT A^GATCT
334 AGATCT
BpuAI 465 GAAGAC GAAGAC(2/6)
1306 GAAGAC
BsaI 244 GAGACC GGTCTC(1/5)
832 GAGACC
BsmBI 210 GAGACG CGTCTC(1/5)
965 GAGACG
BspMI 797 ACCTGC ACCTGC(4/8)
1646 ACCTGC
BssSI 1013 CACGAG CACGAG(-5/-1)
888 CTCGTG
DsaI 682 CCACGG C^C[GA][CT]GG
1180 CCACGG
Eam1105I 246 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1732 GACGCCACGTC
EcoNI 1615 CCTGCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1840 CCTCTAGAAGG
EcoT14I 343 CCTTGG C^C[AT][AT]GG
1742 CCTTGG
FauI 958 GCGGG CCCGC(4/6)
1397 GCGGG
HaeII 478 AGCGCT [GA]GCGC^[CT]
1486 GGCGCT
MaeI 1843 CTAG C^TAG
1894 CTAG
PflFI 1233 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1309 GACCATGTC
SfaNI 1626 GATGC GCATC(5/9)
1781 GCATC
SmlI 728 CTCAAG C^T[CT][GA]AG
1900 CTTGAG
StyI 343 CCTTGG C^C[AT][AT]GG
1742 CCTTGG
Tth111I 1233 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1309 GACCATGTC
XcmI 982 CCAGCGTGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1492 CCAGGTTCTGCTTGG
XmnI 549 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1151 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII