##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspI
AviII AvrII BalI BbrPI BbsI
BbvCI BcgI BciVI BclI BglI
BlnI BpuAI BsaAI BsaBI BsaI
BseAI BsiWI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI Eco47III Eco81I EcoO65I EcoRV
FauI FbaI FokI FseI FspI
HgaI HindIII KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI StuI SwaI
TaqI Tth111I TthHB8I Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
AccI 156 GTAGAC GT^[AC][GT]AC
AcyI 48 GGCGCC G[GA]^CG[CT]C
AflII 1627 CTTAAG C^TTAAG
AhdI 159 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 496 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1092 ATTAAT AT^TAAT
AsnI 1092 ATTAAT AT^TAAT
Asp700I 776 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 159 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 59 CCCGAG C^[CT]CG[GA]G
BamHI 41 GGATCC G^GATCC
BanI 48 GGCGCC G^G[CT][GA]CC
BbeI 48 GGCGCC GGCGC^C
BfrI 1627 CTTAAG C^TTAAG
BglII 963 AGATCT A^GATCT
BlpI 17 GCTGAGC GC^T[ACGT]AGC
BpmI 591 CTGGAG CTGGAG(16/14)
Bpu1102I 17 GCTGAGC GC^T[ACGT]AGC
BsaHI 48 GGCGCC G[GA]^CG[CT]C
BsaWI 374 ACCGGA [AT]^CCGG[AT]
BsiEI 67 CGGCCG CG[GA][CT]^CG
BsmBI 296 CGTCTC CGTCTC(1/5)
BsmFI 106 GGGAC GGGAC(10/14)
BsmI 119 GAATGC GAATGC(1/-1)
BsoBI 59 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1563 TGTACA T^GTACA
BspHI 1672 TCATGA T^CATGA
BsrFI 462 ACCGGC [GA]^CCGG[CT]
BsrGI 1563 TGTACA T^GTACA
CelII 17 GCTGAGC GC^T[ACGT]AGC
Cfr10I 462 ACCGGC [GA]^CCGG[CT]
DraII 180 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 67 CGGCCG [CT]^GGCC[GA]
EagI 67 CGGCCG C^GGCCG
Eam1105I 159 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 67 CGGCCG C^GGCCG
Eco52I 67 CGGCCG C^GGCCG
EcoNI 95 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 180 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1193 CCTTGG C^C[AT][AT]GG
EcoT22I 1343 ATGCAT ATGCA^T
HaeII 48 GGCGCC [GA]GCGC^[CT]
Hin1I 48 GGCGCC G[GA]^CG[CT]C
HincII 793 GTTAAC GT[CT]^[GA]AC
HindII 793 GTTAAC GT[CT]^[GA]AC
HpaI 793 GTTAAC GTT^AAC
KasI 48 GGCGCC G^GCGCC
MspA1I 273 CAGCTG C[AC]G^C[GT]G
NarI 48 GGCGCC GG^CGCC
NdeI 735 CATATG CA^TATG
NsiI 1343 ATGCAT ATGCA^T
NspI 1101 GCATGT [GA]CATG^[CT]
PpuMI 180 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1092 ATTAAT AT^TAAT
PvuII 273 CAGCTG CAG^CTG
RcaI 1672 TCATGA T^CATGA
SapI 258 GAAGAGC GCTCTTC(1/4)
SbfI 98 CCTGCAGG CCTGCA^GG
ScaI 547 AGTACT AGT^ACT
SexAI 334 ACCAGGT A^CC[AT]GGT
SfoI 48 GGCGCC GGC^GCC
Sse8387I 98 CCTGCAGG CCTGCA^GG
SspBI 1563 TGTACA T^GTACA
SspI 1089 AATATT AAT^ATT
StyI 1193 CCTTGG C^C[AT][AT]GG
Tsp45I 226 GTGAC ^GT[GC]AC
XcmI 998 CCAACGGAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 776 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 42 GATCC GGATC(4/5)
41 GGATC
AspHI 660 GAGCAC G[AT]GC[AT]^C
1676 GAGCAC
AvaII 181 GGACC G^G[AT]CC
377 GGACC
BanII 54 GAGCCC G[GA]GC[CT]^C
854 GGGCTC
BcnI 45 CCGGG CC^[GC]GG
741 CCCGG
BseRI 9 GAGGAG GAGGAG(10/8)
555 GAGGAG
BsiHKAI 660 GAGCAC G[AT]GC[AT]^C
1676 GAGCAC
BsmAI 531 GAGAC GTCTC(1/5)
297 GTCTC
BstYI 41 GGATCC [GA]^GATC[CT]
963 AGATCT
Cac8I 892 GCTGGC GC[ACGT]^[ACGT]GC
1407 GCTTGC
CfoI 49 GCGC GCG^C
89 GCGC
EcoRI 1023 GAATTC G^AATTC
1636 GAATTC
HaeIII 68 GGCC GG^CC
76 GGCC
HhaI 49 GCGC GCG^C
89 GCGC
HinP1I 49 GCGC G^CGC
89 GCGC
MaeIII 226 GTGAC ^GT[ACGT]AC
1264 GTAAC
MflI 41 GGATCC [GA]^GATC[CT]
963 AGATCT
MslI 1588 CATTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1610 CATGCCTGTG
NciI 45 CCGGG CC^[GC]GG
741 CCCGG
PstI 99 CTGCAG CTGCA^G
1569 CTGCAG
SfaNI 149 GATGC GCATC(5/9)
362 GATGC
XbaI 1027 TCTAGA T^CTAGA
1640 TCTAGA
XhoII 41 GGATCC [GA]^GATC[CT]
963 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BfaI BmyI BsaJI BsgI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
TfiI Tru9I TseI Tsp509I TspRI