################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## Acc65I AccII AccIII AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BcnI BfrI BglI BglII BlnI BpuAI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoT22I FseI FspI HaeII HhaI HinP1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI Sse8387I SspBI StuI SwaI TaqI TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 1438 GACGTC GACGT^C AccI 1518 GTAGAC GT^[AC][GT]AC AclI 2005 AACGTT AA^CGTT AcyI 1438 GACGTC G[GA]^CG[CT]C AflIII 1649 ACATGT A^C[GA][CT]GT ApaI 1294 GGGCCC GGGCC^C AspI 1708 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2145 TGGCCA TGG^CCA BamHI 309 GGATCC G^GATCC BciVI 1892 GTATCC GTATCC(6/5) BlpI 557 GCTCAGC GC^T[ACGT]AGC Bpu1102I 557 GCTCAGC GC^T[ACGT]AGC BsaAI 1750 TACGTA [CT]AC^GT[GA] BsaBI 1100 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1438 GACGTC G[GA]^CG[CT]C BsaI 487 GGTCTC GGTCTC(1/5) BsaWI 1072 TCCGGT [AT]^CCGG[AT] BsmI 1332 GAATGC GAATGC(1/-1) BspLU11I 1649 ACATGT A^CATGT BstEII 841 GGTTACC G^GT[ACGT]ACC BstPI 841 GGTTACC G^GT[ACGT]ACC CelII 557 GCTCAGC GC^T[ACGT]AGC EaeI 2145 TGGCCA [CT]^GGCC[GA] EcoO65I 841 GGTTACC G^GT[ACGT]ACC EcoRV 414 GATATC GAT^ATC EcoT14I 1219 CCAAGG C^C[AT][AT]GG FauI 975 CCCGC CCCGC(4/6) HapII 1073 CCGG C^CGG Hin1I 1438 GACGTC G[GA]^CG[CT]C HincII 786 GTCAAC GT[CT]^[GA]AC HindII 786 GTCAAC GT[CT]^[GA]AC HpaII 1073 CCGG C^CGG MamI 1100 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2145 TGGCCA TGG^CCA MscI 2145 TGGCCA TGG^CCA MspI 1073 CCGG C^CGG NdeI 1878 CATATG CA^TATG PflFI 1708 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1952 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 779 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2005 AACGTT AA^CGTT PvuII 858 CAGCTG CAG^CTG SapI 1346 GCTCTTC GCTCTTC(1/4) ScaI 266 AGTACT AGT^ACT SnaBI 1750 TACGTA TAC^GTA StyI 1219 CCAAGG C^C[AT][AT]GG Tth111I 1708 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1162 CCAGCTAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AocI 438 CCTGAGG CC^T[ACGT]AGG 924 CCTGAGG AspHI 1091 GTGCTC G[AT]GC[AT]^C 1376 GAGCAC AvaII 778 GGACC G^G[AT]CC 1953 GGTCC BsiHKAI 1091 GTGCTC G[AT]GC[AT]^C 1376 GAGCAC BspMI 851 ACCTGC ACCTGC(4/8) 255 GCAGGT BsrDI 1641 GCAATG GCAATG(2/0) 1703 GCAATG BstYI 309 GGATCC [GA]^GATC[CT] 2214 AGATCC Bsu36I 438 CCTGAGG CC^T[ACGT]AGG 924 CCTGAGG DraI 2035 TTTAAA TTT^AAA 2061 TTTAAA DraII 47 AGGGCCT [GA]G^G[ACGT]CC[CT] 1952 AGGTCCT Eco81I 438 CCTGAGG CC^T[ACGT]AGG 924 CCTGAGG EcoO109I 47 AGGGCCT [GA]G^G[ACGT]CC[CT] 1952 AGGTCCT HgaI 1708 GACGC GACGC(5/10) 1143 GCGTC MflI 309 GGATCC [GA]^GATC[CT] 2214 AGATCC MspA1I 858 CAGCTG C[AC]G^C[GT]G 976 CCGCTG NheI 495 GCTAGC G^CTAGC 1165 GCTAGC PleI 93 GACTC GAGTC(4/5) 174 GACTC SmlI 120 CTCAAG C^T[CT][GA]AG 390 CTTGAG SphI 619 GCATGC GCATG^C 1613 GCATGC XhoII 309 GGATCC [GA]^GATC[CT] 2214 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI BanII BbvI BclI BfaI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII FbaI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI