##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
Acc65I AccII AccIII AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BanI BbeI
BbrPI BbsI BbvCI BcgI BcnI
BfrI BglI BglII BlnI BpuAI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoT22I
FseI FspI HaeII HhaI HinP1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI Sse8387I SspBI StuI
SwaI TaqI TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 1438 GACGTC GACGT^C
AccI 1518 GTAGAC GT^[AC][GT]AC
AclI 2005 AACGTT AA^CGTT
AcyI 1438 GACGTC G[GA]^CG[CT]C
AflIII 1649 ACATGT A^C[GA][CT]GT
ApaI 1294 GGGCCC GGGCC^C
AspI 1708 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2145 TGGCCA TGG^CCA
BamHI 309 GGATCC G^GATCC
BciVI 1892 GTATCC GTATCC(6/5)
BlpI 557 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 557 GCTCAGC GC^T[ACGT]AGC
BsaAI 1750 TACGTA [CT]AC^GT[GA]
BsaBI 1100 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1438 GACGTC G[GA]^CG[CT]C
BsaI 487 GGTCTC GGTCTC(1/5)
BsaWI 1072 TCCGGT [AT]^CCGG[AT]
BsmI 1332 GAATGC GAATGC(1/-1)
BspLU11I 1649 ACATGT A^CATGT
BstEII 841 GGTTACC G^GT[ACGT]ACC
BstPI 841 GGTTACC G^GT[ACGT]ACC
CelII 557 GCTCAGC GC^T[ACGT]AGC
EaeI 2145 TGGCCA [CT]^GGCC[GA]
EcoO65I 841 GGTTACC G^GT[ACGT]ACC
EcoRV 414 GATATC GAT^ATC
EcoT14I 1219 CCAAGG C^C[AT][AT]GG
FauI 975 CCCGC CCCGC(4/6)
HapII 1073 CCGG C^CGG
Hin1I 1438 GACGTC G[GA]^CG[CT]C
HincII 786 GTCAAC GT[CT]^[GA]AC
HindII 786 GTCAAC GT[CT]^[GA]AC
HpaII 1073 CCGG C^CGG
MamI 1100 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2145 TGGCCA TGG^CCA
MscI 2145 TGGCCA TGG^CCA
MspI 1073 CCGG C^CGG
NdeI 1878 CATATG CA^TATG
PflFI 1708 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1952 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 779 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2005 AACGTT AA^CGTT
PvuII 858 CAGCTG CAG^CTG
SapI 1346 GCTCTTC GCTCTTC(1/4)
ScaI 266 AGTACT AGT^ACT
SnaBI 1750 TACGTA TAC^GTA
StyI 1219 CCAAGG C^C[AT][AT]GG
Tth111I 1708 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1162 CCAGCTAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 438 CCTGAGG CC^T[ACGT]AGG
924 CCTGAGG
AspHI 1091 GTGCTC G[AT]GC[AT]^C
1376 GAGCAC
AvaII 778 GGACC G^G[AT]CC
1953 GGTCC
BsiHKAI 1091 GTGCTC G[AT]GC[AT]^C
1376 GAGCAC
BspMI 851 ACCTGC ACCTGC(4/8)
255 GCAGGT
BsrDI 1641 GCAATG GCAATG(2/0)
1703 GCAATG
BstYI 309 GGATCC [GA]^GATC[CT]
2214 AGATCC
Bsu36I 438 CCTGAGG CC^T[ACGT]AGG
924 CCTGAGG
DraI 2035 TTTAAA TTT^AAA
2061 TTTAAA
DraII 47 AGGGCCT [GA]G^G[ACGT]CC[CT]
1952 AGGTCCT
Eco81I 438 CCTGAGG CC^T[ACGT]AGG
924 CCTGAGG
EcoO109I 47 AGGGCCT [GA]G^G[ACGT]CC[CT]
1952 AGGTCCT
HgaI 1708 GACGC GACGC(5/10)
1143 GCGTC
MflI 309 GGATCC [GA]^GATC[CT]
2214 AGATCC
MspA1I 858 CAGCTG C[AC]G^C[GT]G
976 CCGCTG
NheI 495 GCTAGC G^CTAGC
1165 GCTAGC
PleI 93 GACTC GAGTC(4/5)
174 GACTC
SmlI 120 CTCAAG C^T[CT][GA]AG
390 CTTGAG
SphI 619 GCATGC GCATG^C
1613 GCATGC
XhoII 309 GGATCC [GA]^GATC[CT]
2214 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BanII BbvI BclI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII FbaI Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI