##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AccIII AflII AflIII AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BciVI BclI
BfrI BlnI BsaAI BsaBI BseAI
BsmBI BsmI Bsp1407I BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CpoI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI Ksp632I MamI
MfeI MluI MroI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI RcaI
RsrII SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1663 GACGTC GACGT^C
Acc65I 851 GGTACC G^GTACC
AclI 1162 AACGTT AA^CGTT
AcsI 1762 GAATTC [GA]^AATT[CT]
AgeI 953 ACCGGT A^CCGGT
ApoI 1762 GAATTC [GA]^AATT[CT]
Asp700I 951 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 851 GGTACC G^GTACC
BbvCI 1756 GCTGAGG CCTCAGC(-5/-2)
BlpI 1520 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1520 GCTGAGC GC^T[ACGT]AGC
BsgI 1658 GTGCAG GTGCAG(16/14)
BsiWI 1721 CGTACG C^GTACG
BspDI 1735 ATCGAT AT^CGAT
BsrFI 953 ACCGGT [GA]^CCGG[CT]
BsrI 1712 ACTGG ACTGG(1/-1)
BssSI 1353 CACGAG CACGAG(-5/-1)
BstAPI 1596 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1520 GCTGAGC GC^T[ACGT]AGC
Cfr10I 953 ACCGGT [GA]^CCGG[CT]
ClaI 1735 ATCGAT AT^CGAT
DraI 1137 TTTAAA TTT^AAA
EagI 352 CGGCCG C^GGCCG
EclXI 352 CGGCCG C^GGCCG
Eco52I 352 CGGCCG C^GGCCG
EcoRI 1762 GAATTC G^AATTC
EcoT14I 575 CCATGG C^C[AT][AT]GG
HaeII 392 AGCGCC [GA]GCGC^[CT]
HincII 217 GTCGAC GT[CT]^[GA]AC
HindII 217 GTCGAC GT[CT]^[GA]AC
KpnI 851 GGTACC GGTAC^C
KspI 371 CCGCGG CCGC^GG
NcoI 575 CCATGG C^CATGG
PflMI 1453 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 953 ACCGGT A^CCGGT
Psp1406I 1162 AACGTT AA^CGTT
PvuII 550 CAGCTG CAG^CTG
SacII 371 CCGCGG CCGC^GG
SalI 217 GTCGAC G^TCGAC
SfiI 32 GGCCCACGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 575 CCATGG C^C[AT][AT]GG
TfiI 1500 GAATC G^A[AT]TC
Tsp509I 1763 AATT ^AATT
Van91I 1453 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 919 TCTAGA T^CTAGA
XmnI 951 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BalI 449 TGGCCA TGG^CCA
770 TGGCCA
BbsI 1716 GAAGAC GAAGAC(2/6)
649 GTCTTC
BfaI 920 CTAG C^TAG
1274 CTAG
BglII 668 AGATCT A^GATCT
709 AGATCT
BpuAI 1716 GAAGAC GAAGAC(2/6)
649 GTCTTC
BsaI 486 GAGACC GGTCTC(1/5)
700 GAGACC
BsaWI 953 ACCGGT [AT]^CCGG[AT]
1034 ACCGGA
BsiEI 352 CGGCCG CG[GA][CT]^CG
427 CGGTCG
BspMI 64 GCAGGT ACCTGC(4/8)
645 GCAGGT
HphI 514 GGTGA GGTGA(8/7)
731 TCACC
MaeI 920 CTAG C^TAG
1274 CTAG
MluNI 449 TGGCCA TGG^CCA
770 TGGCCA
MscI 449 TGGCCA TGG^CCA
770 TGGCCA
MspA1I 371 CCGCGG C[AC]G^C[GT]G
550 CAGCTG
PaeR7I 259 CTCGAG C^TCGAG
358 CTCGAG
SacI 170 GAGCTC GAGCT^C
1038 GAGCTC
SfaNI 760 GATGC GCATC(5/9)
580 GCATC
XhoI 259 CTCGAG C^TCGAG
358 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbvI
BcgI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I XhoII