##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaLI ApoI AscI AseI
AsnI AspEI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BclI
BfrI BlnI BsaBI BsaI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MslI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TthHB8I Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 820 GGTACC G^GTACC
AcyI 690 GACGCC G[GA]^CG[CT]C
AocI 1241 CCTCAGG CC^T[ACGT]AGG
Asp718I 820 GGTACC G^GTACC
AspI 1066 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 909 TGGCCA TGG^CCA
BbrPI 1724 CACGTG CAC^GTG
BglII 674 AGATCT A^GATCT
BsaAI 1724 CACGTG [CT]AC^GT[GA]
BsaHI 690 GACGCC G[GA]^CG[CT]C
BsgI 835 GTGCAG GTGCAG(16/14)
BsiEI 49 CGGTCG CG[GA][CT]^CG
BsmI 1006 GAATGC GAATGC(1/-1)
BsrBI 46 GAGCGG CCGCTC(-3/-3)
BsrDI 1762 CATTGC GCAATG(2/0)
BstYI 674 AGATCT [GA]^GATC[CT]
Bsu36I 1241 CCTCAGG CC^T[ACGT]AGG
EaeI 909 TGGCCA [CT]^GGCC[GA]
EarI 1525 CTCTTC CTCTTC(1/4)
Eco81I 1241 CCTCAGG CC^T[ACGT]AGG
EcoRV 730 GATATC GAT^ATC
HaeII 628 AGCGCC [GA]GCGC^[CT]
Hin1I 690 GACGCC G[GA]^CG[CT]C
KpnI 820 GGTACC GGTAC^C
Ksp632I 1525 CTCTTC CTCTTC(1/4)
MflI 674 AGATCT [GA]^GATC[CT]
MluNI 909 TGGCCA TGG^CCA
MscI 909 TGGCCA TGG^CCA
NaeI 638 GCCGGC GCC^GGC
NcoI 85 CCATGG C^CATGG
NgoMIV 638 GCCGGC G^CCGGC
NspI 248 ACATGC [GA]CATG^[CT]
PflFI 1066 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1724 CACGTG CAC^GTG
PmlI 1724 CACGTG CAC^GTG
SfcI 981 CTACAG C^T[GA][CT]AG
SmaI 1570 CCCGGG CCC^GGG
SmlI 63 CTCAAG C^T[CT][GA]AG
StuI 977 AGGCCT AGG^CCT
Tsp509I 1780 AATT ^AATT
Tth111I 1066 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1610 CCAGTGTGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 674 AGATCT [GA]^GATC[CT]
XmaCI 1570 CCCGGG C^CCGGG
XmaI 1570 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 80 CGCG CG^CG
761 CGCG
AccIII 420 TCCGGA T^CCGGA
1073 TCCGGA
AlwNI 113 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
712 CAGAGCCTG
ApaI 1547 GGGCCC GGGCC^C
1714 GGGCCC
Asp700I 165 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
672 GAAGATCTTC
AvaI 1570 CCCGGG C^[CT]CG[GA]G
1678 CCCGAG
BbsI 703 GAAGAC GAAGAC(2/6)
1757 GAAGAC
BfaI 358 CTAG C^TAG
1399 CTAG
BglI 61 GCCTCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1666 GCCGGGAGGGC
BpmI 553 CTGGAG CTGGAG(16/14)
1060 CTGGAG
BpuAI 703 GAAGAC GAAGAC(2/6)
1757 GAAGAC
BsaWI 420 TCCGGA [AT]^CCGG[AT]
1073 TCCGGA
BseAI 420 TCCGGA T^CCGGA
1073 TCCGGA
BsoBI 1570 CCCGGG C^[CT]CG[GA]G
1678 CCCGAG
BspEI 420 TCCGGA T^CCGGA
1073 TCCGGA
BspMI 1161 ACCTGC ACCTGC(4/8)
831 GCAGGT
BsrFI 340 ACCGGC [GA]^CCGG[CT]
638 GCCGGC
BstUI 80 CGCG CG^CG
761 CGCG
Cfr10I 340 ACCGGC [GA]^CCGG[CT]
638 GCCGGC
DpnII 399 GATC ^GATC
675 GATC
DrdI 2 GACAGAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
42 GACAGAGCGGTC
DsaI 85 CCATGG C^C[GA][CT]GG
1493 CCACGG
MaeI 358 CTAG C^TAG
1399 CTAG
MaeII 989 ACGT A^CGT
1725 ACGT
MboI 399 GATC ^GATC
675 GATC
MroI 420 TCCGGA T^CCGGA
1073 TCCGGA
MvnI 80 CGCG CG^CG
761 CGCG
NdeII 399 GATC ^GATC
675 GATC
Sau3AI 399 GATC ^GATC
675 GATC
TfiI 109 GATTC G^A[AT]TC
296 GAATC
XmnI 165 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
672 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanI BanII BbvI BcnI BlpI
BmyI Bpu1102I BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CelII CfoI Cfr13I DdeI DraII
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PleI PpuMI PvuII
RsaI SacI Sau96I ScrFI SfaNI
StyI Tru9I TseI Tsp45I TspRI