##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII BbrPI BciVI BclI
BfrI BglII BsaAI BsaBI BsaWI
BseAI BsiHKAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
BssSI BstAPI BstBI BstXI Bsu36I
ClaI CpoI DraI DraIII Eam1105I
EarI Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MamI MfeI MluI MroI MseI
MslI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SwaI Tru9I Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1870 GTATAC GT^[AC][GT]AC
BalI 949 TGGCCA TGG^CCA
BamHI 1466 GGATCC G^GATCC
BbvCI 788 GCTGAGG CCTCAGC(-5/-2)
BcgI 1840 CGAGGGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 984 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 984 GCTCAGC GC^T[ACGT]AGC
BsaI 163 GGTCTC GGTCTC(1/5)
BsgI 702 GTGCAG GTGCAG(16/14)
BsiEI 3 CGGCCG CG[GA][CT]^CG
BsmBI 323 CGTCTC CGTCTC(1/5)
Bsp1407I 867 TGTACA T^GTACA
BsrGI 867 TGTACA T^GTACA
Bst1107I 1870 GTATAC GTA^TAC
BstEII 1030 GGTGACC G^GT[ACGT]ACC
BstPI 1030 GGTGACC G^GT[ACGT]ACC
BstZ17I 1870 GTATAC GTA^TAC
CelII 984 GCTCAGC GC^T[ACGT]AGC
DrdI 315 GACAACCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3 CGGCCG C^GGCCG
EclXI 3 CGGCCG C^GGCCG
Eco52I 3 CGGCCG C^GGCCG
EcoO65I 1030 GGTGACC G^GT[ACGT]ACC
KspI 407 CCGCGG CCGC^GG
MluNI 949 TGGCCA TGG^CCA
MscI 949 TGGCCA TGG^CCA
NcoI 1528 CCATGG C^CATGG
NspI 989 GCATGT [GA]CATG^[CT]
SacII 407 CCGCGG CCGC^GG
SspBI 867 TGTACA T^GTACA
SspI 1750 AATATT AAT^ATT
XbaI 826 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 1135 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1656 CAGGGCCTG
Aor51HI 236 AGCGCT AGC^GCT
796 AGCGCT
AvrII 1383 CCTAGG C^CTAGG
1851 CCTAGG
BbsI 525 GAAGAC GAAGAC(2/6)
1319 GAAGAC
BglI 5 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1573 GCCTTCAAGGC
BlnI 1383 CCTAGG C^CTAGG
1851 CCTAGG
BpmI 1451 CTCCAG CTGGAG(16/14)
435 CTGGAG
BpuAI 525 GAAGAC GAAGAC(2/6)
1319 GAAGAC
BspMI 694 ACCTGC ACCTGC(4/8)
1728 ACCTGC
BsrBI 127 CCGCTC CCGCTC(-3/-3)
225 CCGCTC
BstYI 439 AGATCC [GA]^GATC[CT]
1466 GGATCC
Eco47III 236 AGCGCT AGC^GCT
796 AGCGCT
MflI 439 AGATCC [GA]^GATC[CT]
1466 GGATCC
PpuMI 1558 AGGACCC [GA]G^G[AT]CC[CT]
1634 GGGTCCT
PstI 818 CTGCAG CTGCA^G
1362 CTGCAG
PvuII 102 CAGCTG CAG^CTG
144 CAGCTG
SfcI 818 CTGCAG C^T[GA][CT]AG
1362 CTGCAG
SmaI 604 CCCGGG CCC^GGG
1110 CCCGGG
TaqI 498 TCGA T^CGA
649 TCGA
Tsp509I 1768 AATT ^AATT
1780 AATT
TthHB8I 498 TCGA T^CGA
649 TCGA
XhoII 439 AGATCC [GA]^GATC[CT]
1466 GGATCC
XmaCI 604 CCCGGG C^CCGGG
1110 CCCGGG
XmaI 604 CCCGGG C^CCGGG
1110 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BanI
BanII BbeI BbvI BcnI BfaI
BmyI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI StuI
StyI TfiI TseI Tsp45I TspRI
Van91I XcmI