##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BsaAI BsaHI BsaI BsaWI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AfaI 1871 GTAC GT^AC
AlwI 1579 GGATC GGATC(4/5)
Aor51HI 88 AGCGCT AGC^GCT
AspHI 1021 GAGCAC G[AT]GC[AT]^C
AvaI 97 CCCGGG C^[CT]CG[GA]G
AvrII 422 CCTAGG C^CTAGG
BbsI 815 GAAGAC GAAGAC(2/6)
BlnI 422 CCTAGG C^CTAGG
BlpI 1336 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1336 GCTCAGC GC^T[ACGT]AGC
BpuAI 815 GAAGAC GAAGAC(2/6)
BsaBI 1142 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1088 GAGGAG GAGGAG(10/8)
BsgI 1751 CTGCAC GTGCAG(16/14)
BsiEI 44 CGGCCG CG[GA][CT]^CG
BsiHKAI 1021 GAGCAC G[AT]GC[AT]^C
BsoBI 97 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1870 TGTACA T^GTACA
BspMI 404 GCAGGT ACCTGC(4/8)
BsrDI 1188 GCAATG GCAATG(2/0)
BsrGI 1870 TGTACA T^GTACA
BssHII 2 GCGCGC G^CGCGC
BstYI 1579 GGATCT [GA]^GATC[CT]
CelII 1336 GCTCAGC GC^T[ACGT]AGC
DpnII 1580 GATC ^GATC
DraII 1658 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 120 CCGCGG C^C[GA][CT]GG
EagI 44 CGGCCG C^GGCCG
EclXI 44 CGGCCG C^GGCCG
Eco47III 88 AGCGCT AGC^GCT
Eco52I 44 CGGCCG C^GGCCG
EcoO109I 1658 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 366 GATATC GAT^ATC
KspI 120 CCGCGG CCGC^GG
MaeII 147 ACGT A^CGT
MaeIII 1885 GTGAC ^GT[ACGT]AC
MamI 1142 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1580 GATC ^GATC
MflI 1579 GGATCT [GA]^GATC[CT]
MslI 626 CATACGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 1580 GATC ^GATC
NheI 974 GCTAGC G^CTAGC
PflMI 897 CCAACTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1658 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 1871 GTAC GT^AC
SacII 120 CCGCGG CCGC^GG
Sau3AI 1580 GATC ^GATC
SbfI 1126 CCTGCAGG CCTGCA^GG
SmaI 97 CCCGGG CCC^GGG
SmlI 601 CTTGAG C^T[CT][GA]AG
Sse8387I 1126 CCTGCAGG CCTGCA^GG
SspBI 1870 TGTACA T^GTACA
Tsp45I 1885 GTGAC ^GT[GC]AC
Van91I 897 CCAACTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1579 GGATCT [GA]^GATC[CT]
XmaCI 97 CCCGGG C^CCGGG
XmaI 97 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BanII 196 GGGCTC G[GA]GC[CT]^C
1307 GAGCCC
BcnI 97 CCCGG CC^[GC]GG
98 CCGGG
BsmAI 253 GAGAC GTCTC(1/5)
640 GTCTC
BstF5I 943 GGATG GGATG(2/0)
1372 GGATG
EaeI 44 CGGCCG [CT]^GGCC[GA]
1477 TGGCCG
EarI 1620 CTCTTC CTCTTC(1/4)
1153 GAAGAG
EcoT14I 422 CCTAGG C^C[AT][AT]GG
1280 CCTTGG
FauI 126 CCCGC CCCGC(4/6)
203 GCGGG
FokI 943 GGATG GGATG(9/13)
1372 GGATG
HaeII 88 AGCGCT [GA]GCGC^[CT]
106 GGCGCT
HapII 43 CCGG C^CGG
98 CCGG
HpaII 43 CCGG C^CGG
98 CCGG
HphI 395 GGTGA GGTGA(8/7)
646 GGTGA
Ksp632I 1620 CTCTTC CTCTTC(1/4)
1153 GAAGAG
MspA1I 120 CCGCGG C[AC]G^C[GT]G
135 CCGCTG
MspI 43 CCGG C^CGG
98 CCGG
NciI 97 CCCGG CC^[GC]GG
98 CCGGG
NdeI 1401 CATATG CA^TATG
1664 CATATG
PstI 276 CTGCAG CTGCA^G
1127 CTGCAG
StyI 422 CCTAGG C^C[AT][AT]GG
1280 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
AvaII BbvI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI EcoRII Fnu4HI
HaeIII HhaI HinP1I HinfI ItaI
MaeI MboII MnlI MseI MvaI
MvnI MwoI NlaIII NlaIV PleI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I