##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspHI AspI AvrII BamHI
BanII BbeI BcgI BciVI BclI
BglI BlnI BlpI Bpu1102I BsaI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CpoI DraI DraII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI FbaI
FseI HpaI KasI KpnI KspI
MluI MroI MspA1I MvnI NarI
NheI NotI NruI NspV PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 78 GACGTC GACGT^C
AccI 2374 GTCTAC GT^[AC][GT]AC
AcyI 78 GACGTC G[GA]^CG[CT]C
AflII 2360 CTTAAG C^TTAAG
AflIII 2065 ACGTGT A^C[GA][CT]GT
AhdI 2225 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1033 CCTTAGG CC^T[ACGT]AGG
AspEI 2225 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 41 TGCGCA TGC^GCA
BanI 166 GGTGCC G^G[CT][GA]CC
BbrPI 2064 CACGTG CAC^GTG
BbvCI 1584 GCTGAGG CCTCAGC(-5/-2)
BfrI 2360 CTTAAG C^TTAAG
BmyI 88 GGGCAC G[AGT]GC[ACT]^C
BsaAI 2064 CACGTG [CT]AC^GT[GA]
BsaBI 107 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 78 GACGTC G[GA]^CG[CT]C
BsaWI 411 ACCGGA [AT]^CCGG[AT]
BsmI 792 GAATGC GAATGC(1/-1)
Bsp1286I 88 GGGCAC G[AGT]GC[ACT]^C
BspDI 2517 ATCGAT AT^CGAT
BsrFI 169 GCCGGC [GA]^CCGG[CT]
Bsu36I 1033 CCTTAGG CC^T[ACGT]AGG
Cfr10I 169 GCCGGC [GA]^CCGG[CT]
ClaI 2517 ATCGAT AT^CGAT
DraIII 45 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 433 CCATGG C^C[GA][CT]GG
Eam1105I 2225 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1033 CCTTAGG CC^T[ACGT]AGG
EcoRV 1257 GATATC GAT^ATC
EcoT22I 822 ATGCAT ATGCA^T
FauI 73 GCGGG CCCGC(4/6)
FspI 41 TGCGCA TGC^GCA
HaeII 901 AGCGCC [GA]GCGC^[CT]
Hin1I 78 GACGTC G[GA]^CG[CT]C
HindIII 1224 AAGCTT A^AGCTT
MamI 107 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2445 CAATTG C^AATTG
MunI 2445 CAATTG C^AATTG
NaeI 169 GCCGGC GCC^GGC
NcoI 433 CCATGG C^CATGG
NdeI 400 CATATG CA^TATG
NgoMIV 169 GCCGGC G^CCGGC
NsiI 822 ATGCAT ATGCA^T
PacI 2277 TTAATTAA TTAAT^TAA
PmaCI 2064 CACGTG CAC^GTG
PmlI 2064 CACGTG CAC^GTG
PstI 2208 CTGCAG CTGCA^G
SmaI 68 CCCGGG CCC^GGG
StuI 1832 AGGCCT AGG^CCT
XmaCI 68 CCCGGG C^CCGGG
XmaI 68 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 1043 AAATTC [GA]^AATT[CT]
2560 GAATTT
AlwNI 1249 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2017 CAGTACCTG
ApoI 1043 AAATTC [GA]^AATT[CT]
2560 GAATTT
AvaI 68 CCCGGG C^[CT]CG[GA]G
1906 CTCGGG
AvaII 309 GGACC G^G[AT]CC
2054 GGACC
BalI 28 TGGCCA TGG^CCA
1576 TGGCCA
BglII 854 AGATCT A^GATCT
1484 AGATCT
BpmI 886 CTGGAG CTGGAG(16/14)
1839 CTGGAG
BseRI 1398 GAGGAG GAGGAG(10/8)
2085 GAGGAG
BsgI 62 CTGCAC GTGCAG(16/14)
2455 GTGCAG
BsmAI 23 GTCTC GTCTC(1/5)
1356 GTCTC
BsmFI 76 GGGAC GGGAC(10/14)
387 GGGAC
BsoBI 68 CCCGGG C^[CT]CG[GA]G
1906 CTCGGG
Bsp1407I 248 TGTACA T^GTACA
2403 TGTACA
BsrGI 248 TGTACA T^GTACA
2403 TGTACA
BstNI 1538 CCTGG CC^[AT]GG
2263 CCTGG
CfoI 42 GCGC GCG^C
902 GCGC
EaeI 28 TGGCCA [CT]^GGCC[GA]
1576 TGGCCA
EarI 2440 CTCTTC CTCTTC(1/4)
1287 GAAGAG
EcoRII 1538 CCTGG ^CC[AT]GG
2263 CCTGG
HhaI 42 GCGC GCG^C
902 GCGC
HinP1I 42 GCGC G^CGC
902 GCGC
HincII 3 GTCAAC GT[CT]^[GA]AC
1566 GTTGAC
HindII 3 GTCAAC GT[CT]^[GA]AC
1566 GTTGAC
Ksp632I 2440 CTCTTC CTCTTC(1/4)
1287 GAAGAG
MaeII 79 ACGT A^CGT
2065 ACGT
MluNI 28 TGGCCA TGG^CCA
1576 TGGCCA
MscI 28 TGGCCA TGG^CCA
1576 TGGCCA
MslI 983 CACTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1941 CATTGGAATG
MvaI 1538 CCTGG CC^[AT]GG
2263 CCTGG
MwoI 64 GCACCCCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1122 GCCAATCGTGC
NspI 820 ACATGC [GA]CATG^[CT]
824 GCATGT
SmlI 1668 CTTGAG C^T[CT][GA]AG
2360 CTTAAG
SspBI 248 TGTACA T^GTACA
2403 TGTACA
SspI 1068 AATATT AAT^ATT
1137 AATATT
Tsp45I 580 GTGAC ^GT[GC]AC
2233 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AseI
AsnI Asp700I BbsI BbvI BcnI
BfaI BpuAI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstYI Cac8I
Cfr13I DdeI DpnII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspI NciI NdeII NlaIII NlaIV
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII XmnI