##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AhdI AlwI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
BamHI BbeI BbvCI BcgI BciVI
BclI BfrI BglI BglII BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoRI EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MflI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PmeI
PshAI PshBI Psp1406I PstI PvuI
RsrII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccII 121 CGCG CG^CG
AcsI 192 AAATTC [GA]^AATT[CT]
AflIII 1458 ACACGT A^C[GA][CT]GT
AgeI 817 ACCGGT A^CCGGT
Alw44I 618 GTGCAC G^TGCAC
AlwNI 497 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 618 GTGCAC G^TGCAC
ApoI 192 AAATTC [GA]^AATT[CT]
Asp700I 200 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 409 TGCGCA TGC^GCA
AvrII 1556 CCTAGG C^CTAGG
BalI 487 TGGCCA TGG^CCA
BbrPI 1459 CACGTG CAC^GTG
BlnI 1556 CCTAGG C^CTAGG
BlpI 1412 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1412 GCTGAGC GC^T[ACGT]AGC
BsaAI 1459 CACGTG [CT]AC^GT[GA]
BsaI 1215 GGTCTC GGTCTC(1/5)
BsaWI 817 ACCGGT [AT]^CCGG[AT]
BsgI 956 GTGCAG GTGCAG(16/14)
BsiEI 6 CGGCCG CG[GA][CT]^CG
BsmBI 71 CGTCTC CGTCTC(1/5)
BsmI 885 GCATTC GAATGC(1/-1)
BspHI 208 TCATGA T^CATGA
BstEII 455 GGTCACC G^GT[ACGT]ACC
BstPI 455 GGTCACC G^GT[ACGT]ACC
BstUI 121 CGCG CG^CG
BstXI 856 CCAAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1412 GCTGAGC GC^T[ACGT]AGC
DpnII 653 GATC ^GATC
EagI 6 CGGCCG C^GGCCG
EclXI 6 CGGCCG C^GGCCG
Eco52I 6 CGGCCG C^GGCCG
EcoO65I 455 GGTCACC G^GT[ACGT]ACC
FspI 409 TGCGCA TGC^GCA
HaeII 1297 GGCGCT [GA]GCGC^[CT]
KspI 120 CCGCGG CCGC^GG
MboI 653 GATC ^GATC
MluNI 487 TGGCCA TGG^CCA
MscI 487 TGGCCA TGG^CCA
MslI 1531 CACCGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 121 CGCG CG^CG
NaeI 607 GCCGGC GCC^GGC
NdeII 653 GATC ^GATC
NgoMIV 607 GCCGGC G^CCGGC
PinAI 817 ACCGGT A^CCGGT
PmaCI 1459 CACGTG CAC^GTG
PmlI 1459 CACGTG CAC^GTG
PpuMI 1559 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 208 TCATGA T^CATGA
SacII 120 CCGCGG CCGC^GG
SapI 1394 GAAGAGC GCTCTTC(1/4)
Sau3AI 653 GATC ^GATC
ScaI 781 AGTACT AGT^ACT
SfaNI 262 GCATC GCATC(5/9)
SfcI 23 CTACAG C^T[GA][CT]AG
SmaI 421 CCCGGG CCC^GGG
SphI 1536 GCATGC GCATG^C
TfiI 1152 GATTC G^A[AT]TC
Tsp509I 193 AATT ^AATT
XmaCI 421 CCCGGG C^CCGGG
XmaI 421 CCCGGG C^CCGGG
XmnI 200 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 174 GACGTC GACGT^C
567 GACGTC
AocI 985 CCTCAGG CC^T[ACGT]AGG
1563 CCTCAGG
BanI 417 GGTGCC G^G[CT][GA]CC
1043 GGCACC
BfaI 363 CTAG C^TAG
1557 CTAG
BsrBI 218 CCGCTC CCGCTC(-3/-3)
224 CCGCTC
BsrDI 562 GCAATG GCAATG(2/0)
1107 GCAATG
Bsu36I 985 CCTCAGG CC^T[ACGT]AGG
1563 CCTCAGG
CfoI 410 GCGC GCG^C
1298 GCGC
DsaI 120 CCGCGG C^C[GA][CT]GG
874 CCGTGG
EarI 1394 GAAGAG CTCTTC(1/4)
1607 GAAGAG
Eco81I 985 CCTCAGG CC^T[ACGT]AGG
1563 CCTCAGG
EcoNI 143 CCTCTCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1545 CCTGTGGCAGG
EcoRV 760 GATATC GAT^ATC
936 GATATC
HgaI 679 GCGTC GACGC(5/10)
973 GCGTC
HhaI 410 GCGC GCG^C
1298 GCGC
HinP1I 410 GCGC G^CGC
1298 GCGC
Ksp632I 1394 GAAGAG CTCTTC(1/4)
1607 GAAGAG
MaeI 363 CTAG C^TAG
1557 CTAG
NspI 406 ACATGC [GA]CATG^[CT]
1536 GCATGC
PvuII 638 CAGCTG CAG^CTG
1004 CAGCTG
SacI 369 GAGCTC GAGCT^C
1404 GAGCTC
SmlI 336 CTTGAG C^T[CT][GA]AG
1033 CTTGAG
StuI 397 AGGCCT AGG^CCT
1553 AGGCCT
TaqI 320 TCGA T^CGA
451 TCGA
TthHB8I 320 TCGA T^CGA
451 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I Cfr10I Cfr13I DdeI DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII Hin1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
StyI Tru9I TseI Tsp45I TspRI
XcmI