################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AhdI AlwI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI BamHI BbeI BbvCI BcgI BciVI BclI BfrI BglI BglII BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstYI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoRI EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI MamI MfeI MflI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmeI PshAI PshBI Psp1406I PstI PvuI RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccII 121 CGCG CG^CG AcsI 192 AAATTC [GA]^AATT[CT] AflIII 1458 ACACGT A^C[GA][CT]GT AgeI 817 ACCGGT A^CCGGT Alw44I 618 GTGCAC G^TGCAC AlwNI 497 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 618 GTGCAC G^TGCAC ApoI 192 AAATTC [GA]^AATT[CT] Asp700I 200 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 409 TGCGCA TGC^GCA AvrII 1556 CCTAGG C^CTAGG BalI 487 TGGCCA TGG^CCA BbrPI 1459 CACGTG CAC^GTG BlnI 1556 CCTAGG C^CTAGG BlpI 1412 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1412 GCTGAGC GC^T[ACGT]AGC BsaAI 1459 CACGTG [CT]AC^GT[GA] BsaI 1215 GGTCTC GGTCTC(1/5) BsaWI 817 ACCGGT [AT]^CCGG[AT] BsgI 956 GTGCAG GTGCAG(16/14) BsiEI 6 CGGCCG CG[GA][CT]^CG BsmBI 71 CGTCTC CGTCTC(1/5) BsmI 885 GCATTC GAATGC(1/-1) BspHI 208 TCATGA T^CATGA BstEII 455 GGTCACC G^GT[ACGT]ACC BstPI 455 GGTCACC G^GT[ACGT]ACC BstUI 121 CGCG CG^CG BstXI 856 CCAAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1412 GCTGAGC GC^T[ACGT]AGC DpnII 653 GATC ^GATC EagI 6 CGGCCG C^GGCCG EclXI 6 CGGCCG C^GGCCG Eco52I 6 CGGCCG C^GGCCG EcoO65I 455 GGTCACC G^GT[ACGT]ACC FspI 409 TGCGCA TGC^GCA HaeII 1297 GGCGCT [GA]GCGC^[CT] KspI 120 CCGCGG CCGC^GG MboI 653 GATC ^GATC MluNI 487 TGGCCA TGG^CCA MscI 487 TGGCCA TGG^CCA MslI 1531 CACCGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 121 CGCG CG^CG NaeI 607 GCCGGC GCC^GGC NdeII 653 GATC ^GATC NgoMIV 607 GCCGGC G^CCGGC PinAI 817 ACCGGT A^CCGGT PmaCI 1459 CACGTG CAC^GTG PmlI 1459 CACGTG CAC^GTG PpuMI 1559 AGGACCT [GA]G^G[AT]CC[CT] RcaI 208 TCATGA T^CATGA SacII 120 CCGCGG CCGC^GG SapI 1394 GAAGAGC GCTCTTC(1/4) Sau3AI 653 GATC ^GATC ScaI 781 AGTACT AGT^ACT SfaNI 262 GCATC GCATC(5/9) SfcI 23 CTACAG C^T[GA][CT]AG SmaI 421 CCCGGG CCC^GGG SphI 1536 GCATGC GCATG^C TfiI 1152 GATTC G^A[AT]TC Tsp509I 193 AATT ^AATT XmaCI 421 CCCGGG C^CCGGG XmaI 421 CCCGGG C^CCGGG XmnI 200 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AatII 174 GACGTC GACGT^C 567 GACGTC AocI 985 CCTCAGG CC^T[ACGT]AGG 1563 CCTCAGG BanI 417 GGTGCC G^G[CT][GA]CC 1043 GGCACC BfaI 363 CTAG C^TAG 1557 CTAG BsrBI 218 CCGCTC CCGCTC(-3/-3) 224 CCGCTC BsrDI 562 GCAATG GCAATG(2/0) 1107 GCAATG Bsu36I 985 CCTCAGG CC^T[ACGT]AGG 1563 CCTCAGG CfoI 410 GCGC GCG^C 1298 GCGC DsaI 120 CCGCGG C^C[GA][CT]GG 874 CCGTGG EarI 1394 GAAGAG CTCTTC(1/4) 1607 GAAGAG Eco81I 985 CCTCAGG CC^T[ACGT]AGG 1563 CCTCAGG EcoNI 143 CCTCTCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1545 CCTGTGGCAGG EcoRV 760 GATATC GAT^ATC 936 GATATC HgaI 679 GCGTC GACGC(5/10) 973 GCGTC HhaI 410 GCGC GCG^C 1298 GCGC HinP1I 410 GCGC G^CGC 1298 GCGC Ksp632I 1394 GAAGAG CTCTTC(1/4) 1607 GAAGAG MaeI 363 CTAG C^TAG 1557 CTAG NspI 406 ACATGC [GA]CATG^[CT] 1536 GCATGC PvuII 638 CAGCTG CAG^CTG 1004 CAGCTG SacI 369 GAGCTC GAGCT^C 1404 GAGCTC SmlI 336 CTTGAG C^T[CT][GA]AG 1033 CTTGAG StuI 397 AGGCCT AGG^CCT 1553 AGGCCT TaqI 320 TCGA T^CGA 451 TCGA TthHB8I 320 TCGA T^CGA 451 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcyI AfaI AluI AspHI AvaI AvaII BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII Hin1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI StyI Tru9I TseI Tsp45I TspRI XcmI