##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BcgI BclI BcnI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI Bsu36I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AhdI 1995 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 607 ATTAAT AT^TAAT
AsnI 607 ATTAAT AT^TAAT
Asp700I 368 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1995 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 576 GAGCAC G[AT]GC[AT]^C
BanI 1525 GGTGCC G^G[CT][GA]CC
BbvCI 521 CCTCAGC CCTCAGC(-5/-2)
BciVI 1478 GGATAC GTATCC(6/5)
BlpI 267 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 267 GCTTAGC GC^T[ACGT]AGC
BsgI 879 CTGCAC GTGCAG(16/14)
BsiHKAI 576 GAGCAC G[AT]GC[AT]^C
Bsp1407I 2048 TGTACA T^GTACA
BsrFI 105 GCCGGT [GA]^CCGG[CT]
BsrGI 2048 TGTACA T^GTACA
Bst1107I 253 GTATAC GTA^TAC
BstYI 192 AGATCC [GA]^GATC[CT]
BstZ17I 253 GTATAC GTA^TAC
CelII 267 GCTTAGC GC^T[ACGT]AGC
Cfr10I 105 GCCGGT [GA]^CCGG[CT]
DraI 224 TTTAAA TTT^AAA
DsaI 152 CCGTGG C^C[GA][CT]GG
Eam1105I 1995 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1286 GAAGAG CTCTTC(1/4)
EcoRI 819 GAATTC G^AATTC
HapII 106 CCGG C^CGG
HpaII 106 CCGG C^CGG
Ksp632I 1286 GAAGAG CTCTTC(1/4)
MfeI 1371 CAATTG C^AATTG
MflI 192 AGATCC [GA]^GATC[CT]
MspI 106 CCGG C^CGG
MunI 1371 CAATTG C^AATTG
PshAI 955 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 607 ATTAAT AT^TAAT
SexAI 787 ACCAGGT A^CC[AT]GGT
SspBI 2048 TGTACA T^GTACA
SspI 2075 AATATT AAT^ATT
TaqI 1552 TCGA T^CGA
TfiI 455 GATTC G^A[AT]TC
TspRI 937 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 1552 TCGA T^CGA
XcmI 1057 CCAAGGACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 192 AGATCC [GA]^GATC[CT]
XmnI 368 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 253 GTATAC GT^[AC][GT]AC
1150 GTCTAC
AcsI 819 GAATTC [GA]^AATT[CT]
1869 AAATTT
AlwI 193 GATCC GGATC(4/5)
1258 GATCC
ApoI 819 GAATTC [GA]^AATT[CT]
1869 AAATTT
BpmI 591 CTCCAG CTGGAG(16/14)
912 CTCCAG
BsaI 1423 GAGACC GGTCTC(1/5)
1570 GAGACC
BsmAI 1423 GAGAC GTCTC(1/5)
1570 GAGAC
BsrDI 278 CATTGC GCAATG(2/0)
1948 CATTGC
CfoI 854 GCGC GCG^C
1894 GCGC
EaeI 1331 CGGCCA [CT]^GGCC[GA]
1594 TGGCCG
EcoT14I 1057 CCAAGG C^C[AT][AT]GG
2130 CCAAGG
HaeII 853 AGCGCC [GA]GCGC^[CT]
1893 GGCGCT
HhaI 854 GCGC GCG^C
1894 GCGC
HinP1I 854 GCGC G^CGC
1894 GCGC
HindIII 1675 AAGCTT A^AGCTT
1941 AAGCTT
HphI 710 GGTGA GGTGA(8/7)
2019 GGTGA
PleI 479 GACTC GAGTC(4/5)
832 GACTC
PpuMI 259 AGGACCT [GA]G^G[AT]CC[CT]
1060 AGGACCT
PstI 483 CTGCAG CTGCA^G
1185 CTGCAG
PvuII 143 CAGCTG CAG^CTG
1050 CAGCTG
ScaI 1181 AGTACT AGT^ACT
2042 AGTACT
SmlI 834 CTCAAG C^T[CT][GA]AG
970 CTCAAG
SpeI 1166 ACTAGT A^CTAGT
1238 ACTAGT
StyI 1057 CCAAGG C^C[AT][AT]GG
2130 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbvI BfaI BmyI BsaJI BseRI
BsiYI BslI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI Tru9I TseI Tsp45I Tsp509I
Van91I