##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiWI BsmBI BsoBI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI CelII Cfr10I CpoI
DraIII DrdI Eam1105I Eco47III EcoRI
EcoRV FseI FspI HaeII Hin1I
KasI KpnI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AocI 2045 CCTAAGG CC^T[ACGT]AGG
AspHI 8 GTGCTC G[AT]GC[AT]^C
BalI 957 TGGCCA TGG^CCA
BcgI 1090 CGATGTTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1849 GGATAC GTATCC(6/5)
BclI 2304 TGATCA T^GATCA
BmyI 8 GTGCTC G[AGT]GC[ACT]^C
BsaAI 5 TACGTG [CT]AC^GT[GA]
BsaI 2356 GGTCTC GGTCTC(1/5)
BsiEI 32 CGGCCG CG[GA][CT]^CG
BsiHKAI 8 GTGCTC G[AT]GC[AT]^C
BsmFI 2245 GTCCC GGGAC(10/14)
BsmI 520 GAATGC GAATGC(1/-1)
Bsp1286I 8 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 2500 TGTACA T^GTACA
BspDI 1466 ATCGAT AT^CGAT
BsrDI 1224 GCAATG GCAATG(2/0)
BsrGI 2500 TGTACA T^GTACA
Bst1107I 2342 GTATAC GTA^TAC
BstBI 1703 TTCGAA TT^CGAA
BstYI 16 GGATCT [GA]^GATC[CT]
BstZ17I 2342 GTATAC GTA^TAC
Bsu36I 2045 CCTAAGG CC^T[ACGT]AGG
CfoI 73 GCGC GCG^C
ClaI 1466 ATCGAT AT^CGAT
EagI 32 CGGCCG C^GGCCG
EclXI 32 CGGCCG C^GGCCG
Eco52I 32 CGGCCG C^GGCCG
Eco81I 2045 CCTAAGG CC^T[ACGT]AGG
EcoNI 1840 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1680 ATGCAT ATGCA^T
FauI 28 CCCGC CCCGC(4/6)
FbaI 2304 TGATCA T^GATCA
HgaI 1709 GACGC GACGC(5/10)
HhaI 73 GCGC GCG^C
HinP1I 73 GCGC G^CGC
HindIII 1719 AAGCTT A^AGCTT
HpaI 2378 GTTAAC GTT^AAC
KspI 29 CCGCGG CCGC^GG
MflI 16 GGATCT [GA]^GATC[CT]
MluNI 957 TGGCCA TGG^CCA
MscI 957 TGGCCA TGG^CCA
MslI 103 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1992 CCATGG C^CATGG
NotI 31 GCGGCCGC GC^GGCCGC
NruI 117 TCGCGA TCG^CGA
NsiI 1680 ATGCAT ATGCA^T
NspV 1703 TTCGAA TT^CGAA
SacII 29 CCGCGG CCGC^GG
SapI 914 GAAGAGC GCTCTTC(1/4)
SfuI 1703 TTCGAA TT^CGAA
SspBI 2500 TGTACA T^GTACA
StuI 1837 AGGCCT AGG^CCT
XhoII 16 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1949 GTAGAC GT^[AC][GT]AC
2342 GTATAC
AccII 30 CGCG CG^CG
118 CGCG
AlwI 16 GGATC GGATC(4/5)
1790 GGATC
Asp700I 1604 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2234 GAATAGCTTC
AvaII 1781 GGACC G^G[AT]CC
1926 GGTCC
BbvCI 282 CCTCAGC CCTCAGC(-5/-2)
803 GCTGAGG
BspMI 382 ACCTGC ACCTGC(4/8)
125 GCAGGT
BstEII 140 GGTGACC G^GT[ACGT]ACC
2198 GGTGACC
BstPI 140 GGTGACC G^GT[ACGT]ACC
2198 GGTGACC
BstUI 30 CGCG CG^CG
118 CGCG
BstXI 147 CCAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
792 CCAAGAACGTGG
Cac8I 115 GCTCGC GC[ACGT]^[ACGT]GC
1809 GCCAGC
DraI 1723 TTTAAA TTT^AAA
2434 TTTAAA
DraII 1780 AGGACCC [GA]G^G[ACGT]CC[CT]
1925 GGGTCCT
DsaI 29 CCGCGG C^C[GA][CT]GG
1992 CCATGG
EaeI 32 CGGCCG [CT]^GGCC[GA]
957 TGGCCA
EcoO109I 1780 AGGACCC [GA]G^G[ACGT]CC[CT]
1925 GGGTCCT
EcoO65I 140 GGTGACC G^GT[ACGT]ACC
2198 GGTGACC
HincII 2378 GTTAAC GT[CT]^[GA]AC
2665 GTCAAC
HindII 2378 GTTAAC GT[CT]^[GA]AC
2665 GTCAAC
MaeII 6 ACGT A^CGT
798 ACGT
MvnI 30 CGCG CG^CG
118 CGCG
PpuMI 1780 AGGACCC [GA]G^G[AT]CC[CT]
1925 GGGTCCT
PvuII 348 CAGCTG CAG^CTG
639 CAGCTG
SfcI 1522 CTATAG C^T[GA][CT]AG
1588 CTACAG
TaqI 1467 TCGA T^CGA
1704 TCGA
TfiI 1169 GAATC G^A[AT]TC
1556 GAATC
TthHB8I 1467 TCGA T^CGA
1704 TCGA
XcmI 1651 CCAGAACCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1983 CCAAGGAGGCCATGG
XmnI 1604 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2234 GAATAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI BbsI BbvI BcnI BfaI
BglI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsrI BssKI
BstAPI BstF5I BstNI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp45I Tsp509I TspRI