##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AccI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KspI
MfeI MluI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 16 GACGTC GACGT^C
AccII 33 CGCG CG^CG
AccIII 1293 TCCGGA T^CCGGA
AclI 1162 AACGTT AA^CGTT
AflIII 1581 ACGTGT A^C[GA][CT]GT
AocI 1525 CCTCAGG CC^T[ACGT]AGG
ApaI 163 GGGCCC GGGCC^C
Asp700I 496 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1080 GAGCAC G[AT]GC[AT]^C
AvaI 1091 CCCGGG C^[CT]CG[GA]G
BbrPI 1580 CACGTG CAC^GTG
BbsI 1570 GAAGAC GAAGAC(2/6)
BglI 58 GCCCTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1461 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1461 GCTGAGC GC^T[ACGT]AGC
BpuAI 1570 GAAGAC GAAGAC(2/6)
BsaAI 1580 CACGTG [CT]AC^GT[GA]
BsaWI 1293 TCCGGA [AT]^CCGG[AT]
BseAI 1293 TCCGGA T^CCGGA
BsiHKAI 1080 GAGCAC G[AT]GC[AT]^C
BsmI 500 GCATTC GAATGC(1/-1)
BsoBI 1091 CCCGGG C^[CT]CG[GA]G
BspEI 1293 TCCGGA T^CCGGA
BspMI 1208 ACCTGC ACCTGC(4/8)
BstUI 33 CGCG CG^CG
BstXI 342 CCAAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1677 AGATCC [GA]^GATC[CT]
Bsu36I 1525 CCTCAGG CC^T[ACGT]AGG
CelII 1461 GCTGAGC GC^T[ACGT]AGC
DraIII 1263 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 727 CCATGG C^C[GA][CT]GG
Eco81I 1525 CCTCAGG CC^T[ACGT]AGG
EcoNI 1209 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MflI 1677 AGATCC [GA]^GATC[CT]
MroI 1293 TCCGGA T^CCGGA
MslI 1727 CATTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 33 CGCG CG^CG
NcoI 727 CCATGG C^CATGG
NdeI 805 CATATG CA^TATG
PmaCI 1580 CACGTG CAC^GTG
PmlI 1580 CACGTG CAC^GTG
PpuMI 1256 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1162 AACGTT AA^CGTT
SfaNI 862 GATGC GCATC(5/9)
SmaI 1091 CCCGGG CCC^GGG
SphI 1366 GCATGC GCATG^C
SspI 1535 AATATT AAT^ATT
TaqI 209 TCGA T^CGA
TthHB8I 209 TCGA T^CGA
XhoII 1677 AGATCC [GA]^GATC[CT]
XmaCI 1091 CCCGGG C^CCGGG
XmaI 1091 CCCGGG C^CCGGG
XmnI 496 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 1202 GGTACC G^GTACC
1219 GGTACC
AlwI 1227 GATCC GGATC(4/5)
1678 GATCC
Asp718I 1202 GGTACC G^GTACC
1219 GGTACC
BbeI 140 GGCGCC GGCGC^C
170 GGCGCC
BpmI 223 CTGGAG CTGGAG(16/14)
1306 CTGGAG
BsaBI 829 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1678 GATCCAAATC
BseRI 508 GAGGAG GAGGAG(10/8)
541 GAGGAG
BsgI 1249 CTGCAC GTGCAG(16/14)
1132 GTGCAG
BsmFI 1256 GGGAC GGGAC(10/14)
1619 GGGAC
FauI 156 CCCGC CCCGC(4/6)
1362 GCGGG
KasI 140 GGCGCC G^GCGCC
170 GGCGCC
KpnI 1202 GGTACC GGTAC^C
1219 GGTACC
MamI 829 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1678 GATCCAAATC
MspA1I 232 CAGCTG C[AC]G^C[GT]G
655 CAGCTG
MwoI 25 GCCATGCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
58 GCCCTCTGGGC
NarI 140 GGCGCC GG^CGCC
170 GGCGCC
NspI 703 ACATGC [GA]CATG^[CT]
1366 GCATGC
PflMI 1262 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1299 CCATTTTCTGG
PvuII 232 CAGCTG CAG^CTG
655 CAGCTG
SapI 4 GAAGAGC GCTCTTC(1/4)
483 GAAGAGC
SfoI 140 GGCGCC GGC^GCC
170 GGCGCC
TfiI 834 GAATC G^A[AT]TC
1810 GAATC
Van91I 1262 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1299 CCATTTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwNI ApoI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BsaHI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MspI MvaI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI StyI Tru9I TseI Tsp45I
Tsp509I TspRI