################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AflII AflIII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvrII BamHI BbeI BcgI BclI BfrI BlnI BsaBI BsaHI BsaI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI DraI Eam1105I EcoNI EcoRI EcoRV FbaI FseI Hin1I HindIII HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI PvuI SacI SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AclI 627 AACGTT AA^CGTT AcsI 942 GAATTT [GA]^AATT[CT] Aor51HI 1499 AGCGCT AGC^GCT ApoI 942 GAATTT [GA]^AATT[CT] Asp700I 355 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 895 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1001 CCCGGG C^[CT]CG[GA]G BalI 834 TGGCCA TGG^CCA BbrPI 370 CACGTG CAC^GTG BbsI 1162 GTCTTC GAAGAC(2/6) BbvCI 1532 GCTGAGG CCTCAGC(-5/-2) BciVI 1451 GTATCC GTATCC(6/5) BfaI 968 CTAG C^TAG BglII 1128 AGATCT A^GATCT BlpI 142 GCTCAGC GC^T[ACGT]AGC Bpu1102I 142 GCTCAGC GC^T[ACGT]AGC BpuAI 1162 GTCTTC GAAGAC(2/6) BsaWI 11 TCCGGT [AT]^CCGG[AT] BsoBI 1001 CCCGGG C^[CT]CG[GA]G BspHI 704 TCATGA T^CATGA BsrBI 140 CCGCTC CCGCTC(-3/-3) BsrFI 868 ACCGGC [GA]^CCGG[CT] BstEII 846 GGTCACC G^GT[ACGT]ACC BstPI 846 GGTCACC G^GT[ACGT]ACC CelII 142 GCTCAGC GC^T[ACGT]AGC Cfr10I 868 ACCGGC [GA]^CCGG[CT] CpoI 1441 CGGACCG CG^G[AT]CCG DrdI 412 GACAGAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 538 CGGCCG C^GGCCG EarI 1111 CTCTTC CTCTTC(1/4) EclXI 538 CGGCCG C^GGCCG Eco47III 1499 AGCGCT AGC^GCT Eco52I 538 CGGCCG C^GGCCG EcoO65I 846 GGTCACC G^GT[ACGT]ACC EcoT22I 1292 ATGCAT ATGCA^T Ksp632I 1111 CTCTTC CTCTTC(1/4) KspI 726 CCGCGG CCGC^GG MaeI 968 CTAG C^TAG MluNI 834 TGGCCA TGG^CCA MscI 834 TGGCCA TGG^CCA MseI 431 TTAA T^TAA NcoI 752 CCATGG C^CATGG NsiI 1292 ATGCAT ATGCA^T PflFI 895 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 370 CACGTG CAC^GTG PmlI 370 CACGTG CAC^GTG PpuMI 1120 GGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 627 AACGTT AA^CGTT RcaI 704 TCATGA T^CATGA RsrII 1441 CGGACCG CG^G[AT]CCG SacII 726 CCGCGG CCGC^GG SapI 1110 GCTCTTC GCTCTTC(1/4) SbfI 1551 CCTGCAGG CCTGCA^GG ScaI 425 AGTACT AGT^ACT SmaI 1001 CCCGGG CCC^GGG SmlI 1388 CTCAAG C^T[CT][GA]AG Sse8387I 1551 CCTGCAGG CCTGCA^GG Tru9I 431 TTAA T^TAA Tsp509I 943 AATT ^AATT Tth111I 895 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1001 CCCGGG C^CCGGG XmaI 1001 CCCGGG C^CCGGG XmnI 355 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 426 GTAC GT^AC 1880 GTAC AlwNI 353 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1754 CAGCCTCTG AocI 792 CCTGAGG CC^T[ACGT]AGG 1323 CCTGAGG AspHI 457 GAGCAC G[AT]GC[AT]^C 763 GAGCAC BanII 1330 GAGCCC G[GA]GC[CT]^C 1844 GAGCCC BglI 130 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 209 GCCTCGGCGGC BsaAI 175 TACGTG [CT]AC^GT[GA] 370 CACGTG BsgI 1744 CTGCAC GTGCAG(16/14) 1102 GTGCAG BsiEI 538 CGGCCG CG[GA][CT]^CG 1157 CGACCG BsiHKAI 457 GAGCAC G[AT]GC[AT]^C 763 GAGCAC BsrDI 522 CATTGC GCAATG(2/0) 1862 GCAATG BstAPI 1431 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1564 GCACACGGTGC Bsu36I 792 CCTGAGG CC^T[ACGT]AGG 1323 CCTGAGG DraIII 198 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG 1565 CACACGGTG Eco81I 792 CCTGAGG CC^T[ACGT]AGG 1323 CCTGAGG HgaI 21 GACGC GACGC(5/10) 224 GCGTC HincII 226 GTCAAC GT[CT]^[GA]AC 451 GTTGAC HindII 226 GTCAAC GT[CT]^[GA]AC 451 GTTGAC NspI 1233 GCATGT [GA]CATG^[CT] 1462 ACATGC PleI 997 GACTC GAGTC(4/5) 920 GAGTC RsaI 426 GTAC GT^AC 1880 GTAC StuI 279 AGGCCT AGG^CCT 1506 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AluI AlwI AvaII AviII BanI BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XcmI XhoII