##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BbeI
BcgI BclI BfrI BlnI BsaBI
BsaHI BsaI BseAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI DraI Eam1105I
EcoNI EcoRI EcoRV FbaI FseI
Hin1I HindIII HpaI KasI KpnI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
PvuI SacI SalI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AclI 627 AACGTT AA^CGTT
AcsI 942 GAATTT [GA]^AATT[CT]
Aor51HI 1499 AGCGCT AGC^GCT
ApoI 942 GAATTT [GA]^AATT[CT]
Asp700I 355 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 895 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1001 CCCGGG C^[CT]CG[GA]G
BalI 834 TGGCCA TGG^CCA
BbrPI 370 CACGTG CAC^GTG
BbsI 1162 GTCTTC GAAGAC(2/6)
BbvCI 1532 GCTGAGG CCTCAGC(-5/-2)
BciVI 1451 GTATCC GTATCC(6/5)
BfaI 968 CTAG C^TAG
BglII 1128 AGATCT A^GATCT
BlpI 142 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 142 GCTCAGC GC^T[ACGT]AGC
BpuAI 1162 GTCTTC GAAGAC(2/6)
BsaWI 11 TCCGGT [AT]^CCGG[AT]
BsoBI 1001 CCCGGG C^[CT]CG[GA]G
BspHI 704 TCATGA T^CATGA
BsrBI 140 CCGCTC CCGCTC(-3/-3)
BsrFI 868 ACCGGC [GA]^CCGG[CT]
BstEII 846 GGTCACC G^GT[ACGT]ACC
BstPI 846 GGTCACC G^GT[ACGT]ACC
CelII 142 GCTCAGC GC^T[ACGT]AGC
Cfr10I 868 ACCGGC [GA]^CCGG[CT]
CpoI 1441 CGGACCG CG^G[AT]CCG
DrdI 412 GACAGAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 538 CGGCCG C^GGCCG
EarI 1111 CTCTTC CTCTTC(1/4)
EclXI 538 CGGCCG C^GGCCG
Eco47III 1499 AGCGCT AGC^GCT
Eco52I 538 CGGCCG C^GGCCG
EcoO65I 846 GGTCACC G^GT[ACGT]ACC
EcoT22I 1292 ATGCAT ATGCA^T
Ksp632I 1111 CTCTTC CTCTTC(1/4)
KspI 726 CCGCGG CCGC^GG
MaeI 968 CTAG C^TAG
MluNI 834 TGGCCA TGG^CCA
MscI 834 TGGCCA TGG^CCA
MseI 431 TTAA T^TAA
NcoI 752 CCATGG C^CATGG
NsiI 1292 ATGCAT ATGCA^T
PflFI 895 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 370 CACGTG CAC^GTG
PmlI 370 CACGTG CAC^GTG
PpuMI 1120 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 627 AACGTT AA^CGTT
RcaI 704 TCATGA T^CATGA
RsrII 1441 CGGACCG CG^G[AT]CCG
SacII 726 CCGCGG CCGC^GG
SapI 1110 GCTCTTC GCTCTTC(1/4)
SbfI 1551 CCTGCAGG CCTGCA^GG
ScaI 425 AGTACT AGT^ACT
SmaI 1001 CCCGGG CCC^GGG
SmlI 1388 CTCAAG C^T[CT][GA]AG
Sse8387I 1551 CCTGCAGG CCTGCA^GG
Tru9I 431 TTAA T^TAA
Tsp509I 943 AATT ^AATT
Tth111I 895 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1001 CCCGGG C^CCGGG
XmaI 1001 CCCGGG C^CCGGG
XmnI 355 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 426 GTAC GT^AC
1880 GTAC
AlwNI 353 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1754 CAGCCTCTG
AocI 792 CCTGAGG CC^T[ACGT]AGG
1323 CCTGAGG
AspHI 457 GAGCAC G[AT]GC[AT]^C
763 GAGCAC
BanII 1330 GAGCCC G[GA]GC[CT]^C
1844 GAGCCC
BglI 130 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
209 GCCTCGGCGGC
BsaAI 175 TACGTG [CT]AC^GT[GA]
370 CACGTG
BsgI 1744 CTGCAC GTGCAG(16/14)
1102 GTGCAG
BsiEI 538 CGGCCG CG[GA][CT]^CG
1157 CGACCG
BsiHKAI 457 GAGCAC G[AT]GC[AT]^C
763 GAGCAC
BsrDI 522 CATTGC GCAATG(2/0)
1862 GCAATG
BstAPI 1431 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1564 GCACACGGTGC
Bsu36I 792 CCTGAGG CC^T[ACGT]AGG
1323 CCTGAGG
DraIII 198 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
1565 CACACGGTG
Eco81I 792 CCTGAGG CC^T[ACGT]AGG
1323 CCTGAGG
HgaI 21 GACGC GACGC(5/10)
224 GCGTC
HincII 226 GTCAAC GT[CT]^[GA]AC
451 GTTGAC
HindII 226 GTCAAC GT[CT]^[GA]AC
451 GTTGAC
NspI 1233 GCATGT [GA]CATG^[CT]
1462 ACATGC
PleI 997 GACTC GAGTC(4/5)
920 GAGTC
RsaI 426 GTAC GT^AC
1880 GTAC
StuI 279 AGGCCT AGG^CCT
1506 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
AviII BanI BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII