##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AocI ApoI AscI AseI AsnI
Asp700I AvrII BamHI BbsI BciVI
BfrI BglI BlnI BpuAI BsaI
BsiEI BsiWI BsmBI BspDI BspMI
BsrBI BsrDI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DrdI EagI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FseI MfeI MluI MunI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 74 enzymes) #
###################################
Acc65I 1004 GGTACC G^GTACC
AccI 609 GTCGAC GT^[AC][GT]AC
AccIII 848 TCCGGA T^CCGGA
AcyI 167 GGCGCC G[GA]^CG[CT]C
AflIII 1607 ACATGT A^C[GA][CT]GT
AhdI 1654 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1126 GTGCAC G^TGCAC
ApaLI 1126 GTGCAC G^TGCAC
Asp718I 1004 GGTACC G^GTACC
AspEI 1654 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 346 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 824 TGCGCA TGC^GCA
BalI 1984 TGGCCA TGG^CCA
BbeI 167 GGCGCC GGCGC^C
BbrPI 376 CACGTG CAC^GTG
BbvCI 1093 CCTCAGC CCTCAGC(-5/-2)
BcgI 416 CGAGATTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1370 TGATCA T^GATCA
BglII 701 AGATCT A^GATCT
BlpI 322 GCTGAGC GC^T[ACGT]AGC
BpmI 1042 CTGGAG CTGGAG(16/14)
Bpu1102I 322 GCTGAGC GC^T[ACGT]AGC
BsaAI 376 CACGTG [CT]AC^GT[GA]
BsaBI 1533 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 167 GGCGCC G[GA]^CG[CT]C
BsaWI 848 TCCGGA [AT]^CCGG[AT]
BseAI 848 TCCGGA T^CCGGA
BsmAI 527 GAGAC GTCTC(1/5)
BsmI 1857 GCATTC GAATGC(1/-1)
Bsp1407I 1936 TGTACA T^GTACA
BspEI 848 TCCGGA T^CCGGA
BspHI 665 TCATGA T^CATGA
BspLU11I 1607 ACATGT A^CATGT
BsrGI 1936 TGTACA T^GTACA
BsrI 2053 ACTGG ACTGG(1/-1)
CelII 322 GCTGAGC GC^T[ACGT]AGC
DraI 2100 TTTAAA TTT^AAA
DraIII 1342 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1984 TGGCCA [CT]^GGCC[GA]
Eam1105I 1654 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 550 GAAGAG CTCTTC(1/4)
EcoRV 1597 GATATC GAT^ATC
FbaI 1370 TGATCA T^GATCA
FspI 824 TGCGCA TGC^GCA
Hin1I 167 GGCGCC G[GA]^CG[CT]C
HpaI 91 GTTAAC GTT^AAC
KasI 167 GGCGCC G^GCGCC
KpnI 1004 GGTACC GGTAC^C
Ksp632I 550 GAAGAG CTCTTC(1/4)
KspI 171 CCGCGG CCGC^GG
MamI 1533 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1984 TGGCCA TGG^CCA
MroI 848 TCCGGA T^CCGGA
MscI 1984 TGGCCA TGG^CCA
NaeI 231 GCCGGC GCC^GGC
NarI 167 GGCGCC GG^CGCC
NcoI 557 CCATGG C^CATGG
NdeI 1849 CATATG CA^TATG
NgoMIV 231 GCCGGC G^CCGGC
NheI 1563 GCTAGC G^CTAGC
PaeR7I 524 CTCGAG C^TCGAG
PflFI 346 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 376 CACGTG CAC^GTG
PmlI 376 CACGTG CAC^GTG
RcaI 665 TCATGA T^CATGA
SacII 171 CCGCGG CCGC^GG
SalI 609 GTCGAC G^TCGAC
SapI 550 GAAGAGC GCTCTTC(1/4)
SexAI 1271 ACCAGGT A^CC[AT]GGT
SfoI 167 GGCGCC GGC^GCC
SphI 837 GCATGC GCATG^C
SspBI 1936 TGTACA T^GTACA
Tth111I 346 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 524 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 1167 GATCC GGATC(4/5)
1281 GGATC
Aor51HI 680 AGCGCT AGC^GCT
1205 AGCGCT
ApaI 431 GGGCCC GGGCC^C
746 GGGCCC
BstYI 701 AGATCT [GA]^GATC[CT]
1166 AGATCC
DsaI 171 CCGCGG C^C[GA][CT]GG
557 CCATGG
Eco47III 680 AGCGCT AGC^GCT
1205 AGCGCT
EcoNI 1080 CCTTCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1901 CCTCCCCCAGG
HindIII 1132 AAGCTT A^AGCTT
2083 AAGCTT
HphI 1926 GGTGA GGTGA(8/7)
353 TCACC
MaeII 377 ACGT A^CGT
1346 ACGT
MflI 701 AGATCT [GA]^GATC[CT]
1166 AGATCC
MslI 660 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1140 CATCGTGGTG
NspI 837 GCATGC [GA]CATG^[CT]
1607 ACATGT
PflMI 240 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1272 CCAGGTCCTGG
PleI 1233 GACTC GAGTC(4/5)
1660 GAGTC
ScaI 730 AGTACT AGT^ACT
1046 AGTACT
Tsp509I 913 AATT ^AATT
1326 AATT
Van91I 240 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1272 CCAGGTCCTGG
XhoII 701 AGATCT [GA]^GATC[CT]
1166 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrFI BssKI
BssSI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I XmaCI XmaI