##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BanII BbeI BbrPI BbvCI
BcgI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrGI BssHII BssSI BstAPI BstUI
CelII CfoI ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FauI FseI FspI
HaeII HhaI HinP1I HindIII KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AcyI 417 GACGCC G[GA]^CG[CT]C
AocI 869 CCTAAGG CC^T[ACGT]AGG
Asp700I 1715 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 400 TGGCCA TGG^CCA
BamHI 1330 GGATCC G^GATCC
BanI 1488 GGCACC G^G[CT][GA]CC
BbsI 698 GAAGAC GAAGAC(2/6)
BciVI 1404 GTATCC GTATCC(6/5)
BfaI 1730 CTAG C^TAG
BpuAI 698 GAAGAC GAAGAC(2/6)
BsaHI 417 GACGCC G[GA]^CG[CT]C
BseRI 852 GAGGAG GAGGAG(10/8)
BsgI 1803 GTGCAG GTGCAG(16/14)
BspHI 1159 TCATGA T^CATGA
BspMI 651 ACCTGC ACCTGC(4/8)
BsrBI 286 CCGCTC CCGCTC(-3/-3)
BsrFI 55 GCCGGT [GA]^CCGG[CT]
Bst1107I 1110 GTATAC GTA^TAC
BstBI 1722 TTCGAA TT^CGAA
BstEII 923 GGTAACC G^GT[ACGT]ACC
BstF5I 1450 GGATG GGATG(2/0)
BstPI 923 GGTAACC G^GT[ACGT]ACC
BstXI 1836 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1110 GTATAC GTA^TAC
Bsu36I 869 CCTAAGG CC^T[ACGT]AGG
Cac8I 502 GCTTGC GC[ACGT]^[ACGT]GC
Cfr10I 55 GCCGGT [GA]^CCGG[CT]
DraI 958 TTTAAA TTT^AAA
EaeI 400 TGGCCA [CT]^GGCC[GA]
Eco81I 869 CCTAAGG CC^T[ACGT]AGG
EcoNI 128 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 923 GGTAACC G^GT[ACGT]ACC
EcoRV 1571 GATATC GAT^ATC
EcoT22I 1151 ATGCAT ATGCA^T
FokI 1450 GGATG GGATG(9/13)
HgaI 417 GACGC GACGC(5/10)
Hin1I 417 GACGCC G[GA]^CG[CT]C
MaeI 1730 CTAG C^TAG
MfeI 1900 CAATTG C^AATTG
MluNI 400 TGGCCA TGG^CCA
MscI 400 TGGCCA TGG^CCA
MspA1I 1264 CCGCTG C[AC]G^C[GT]G
MunI 1900 CAATTG C^AATTG
NsiI 1151 ATGCAT ATGCA^T
NspV 1722 TTCGAA TT^CGAA
PaeR7I 1185 CTCGAG C^TCGAG
RcaI 1159 TCATGA T^CATGA
SapI 1875 GAAGAGC GCTCTTC(1/4)
SexAI 781 ACCAGGT A^CC[AT]GGT
SfuI 1722 TTCGAA TT^CGAA
SmlI 1185 CTCGAG C^T[CT][GA]AG
SspI 1526 AATATT AAT^ATT
StuI 448 AGGCCT AGG^CCT
XcmI 1408 CCATGTGGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1185 CTCGAG C^TCGAG
XmnI 1715 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 336 GTAC GT^AC
350 GTAC
AvaI 1 CCCGAG C^[CT]CG[GA]G
1185 CTCGAG
BclI 892 TGATCA T^GATCA
900 TGATCA
BmyI 178 GGGCAC G[AGT]GC[ACT]^C
1487 GGGCAC
BsoBI 1 CCCGAG C^[CT]CG[GA]G
1185 CTCGAG
Bsp1286I 178 GGGCAC G[AGT]GC[ACT]^C
1487 GGGCAC
BspLU11I 671 ACATGT A^CATGT
1234 ACATGT
DraII 381 GGGACCT [GA]G^G[ACGT]CC[CT]
1606 AGGTCCC
DsaI 271 CCATGG C^C[GA][CT]GG
800 CCATGG
EarI 731 GAAGAG CTCTTC(1/4)
1875 GAAGAG
EcoO109I 381 GGGACCT [GA]G^G[ACGT]CC[CT]
1606 AGGTCCC
FbaI 892 TGATCA T^GATCA
900 TGATCA
HapII 56 CCGG C^CGG
166 CCGG
HincII 1480 GTTAAC GT[CT]^[GA]AC
1498 GTTAAC
HindII 1480 GTTAAC GT[CT]^[GA]AC
1498 GTTAAC
HpaI 1480 GTTAAC GTT^AAC
1498 GTTAAC
HpaII 56 CCGG C^CGG
166 CCGG
HphI 1414 GGTGA GGTGA(8/7)
1712 GGTGA
Ksp632I 731 GAAGAG CTCTTC(1/4)
1875 GAAGAG
MspI 56 CCGG C^CGG
166 CCGG
NcoI 271 CCATGG C^CATGG
800 CCATGG
NdeI 551 CATATG CA^TATG
1305 CATATG
PleI 1380 GAGTC GAGTC(4/5)
1813 GAGTC
PpuMI 381 GGGACCT [GA]G^G[AT]CC[CT]
1606 AGGTCCC
RsaI 336 GTAC GT^AC
350 GTAC
TaqI 1186 TCGA T^CGA
1723 TCGA
TthHB8I 1186 TCGA T^CGA
1723 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccI AciI AcsI AflIII AluI
AlwI AlwNI ApoI AvaII BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsmI BsrDI BsrI BssKI
BstNI BstYI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI HaeIII HinfI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII