##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbrPI BbsI BcgI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsoBI BspDI BspEI BspLU11I BspMI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraII DraIII DrdI Eam1105I
Eco47III EcoO109I EcoO65I EcoRV FseI
FspI HincII HindII HindIII HpaI
KpnI MamI MluI MluNI MroI
MscI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 1296 GTCTAC GT^[AC][GT]AC
AcyI 94 GGCGCC G[GA]^CG[CT]C
AflII 769 CTTAAG C^TTAAG
AflIII 412 ACGTGT A^C[GA][CT]GT
AlwNI 270 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1398 CCTTAGG CC^T[ACGT]AGG
Asp700I 370 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 36 GAGCTC G[AT]GC[AT]^C
AvaII 1411 GGTCC G^G[AT]CC
BanII 36 GAGCTC G[GA]GC[CT]^C
BbeI 94 GGCGCC GGCGC^C
BciVI 191 GGATAC GTATCC(6/5)
BclI 287 TGATCA T^GATCA
BfrI 769 CTTAAG C^TTAAG
BsaHI 94 GGCGCC G[GA]^CG[CT]C
BsaI 10 GGTCTC GGTCTC(1/5)
BseRI 280 GAGGAG GAGGAG(10/8)
BsiHKAI 36 GAGCTC G[AT]GC[AT]^C
BsmAI 11 GTCTC GTCTC(1/5)
BspHI 1286 TCATGA T^CATGA
BsrBI 154 CCGCTC CCGCTC(-3/-3)
BsrDI 1614 GCAATG GCAATG(2/0)
BsrFI 174 GCCGGC [GA]^CCGG[CT]
BstXI 539 CCACAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1398 CCTTAGG CC^T[ACGT]AGG
Cfr10I 174 GCCGGC [GA]^CCGG[CT]
Cfr13I 1411 GGTCC G^G[ACGT]CC
EaeI 172 CGGCCG [CT]^GGCC[GA]
EagI 172 CGGCCG C^GGCCG
EarI 157 CTCTTC CTCTTC(1/4)
EclXI 172 CGGCCG C^GGCCG
Eco52I 172 CGGCCG C^GGCCG
Eco81I 1398 CCTTAGG CC^T[ACGT]AGG
EcoNI 1394 CCTCCCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 876 CCATGG C^C[AT][AT]GG
EcoT22I 1713 ATGCAT ATGCA^T
FbaI 287 TGATCA T^GATCA
Hin1I 94 GGCGCC G[GA]^CG[CT]C
KasI 94 GGCGCC G^GCGCC
Ksp632I 157 CTCTTC CTCTTC(1/4)
KspI 31 CCGCGG CCGC^GG
MfeI 730 CAATTG C^AATTG
MunI 730 CAATTG C^AATTG
NaeI 174 GCCGGC GCC^GGC
NarI 94 GGCGCC GG^CGCC
NcoI 876 CCATGG C^CATGG
NgoMIV 174 GCCGGC G^CCGGC
NsiI 1713 ATGCAT ATGCA^T
PleI 418 GACTC GAGTC(4/5)
PstI 921 CTGCAG CTGCA^G
RcaI 1286 TCATGA T^CATGA
SacI 36 GAGCTC GAGCT^C
SacII 31 CCGCGG CCGC^GG
SapI 156 GCTCTTC GCTCTTC(1/4)
Sau96I 1411 GGTCC G^G[ACGT]CC
SfcI 921 CTGCAG C^T[GA][CT]AG
SfoI 94 GGCGCC GGC^GCC
SmlI 769 CTTAAG C^T[CT][GA]AG
StuI 115 AGGCCT AGG^CCT
StyI 876 CCATGG C^C[AT][AT]GG
TaqI 822 TCGA T^CGA
TthHB8I 822 TCGA T^CGA
XcmI 539 CCACAAAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 370 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BbvCI 84 CCTCAGC CCTCAGC(-5/-2)
297 CCTCAGC
BcnI 170 CCCGG CC^[GC]GG
327 CCGGG
BfaI 1605 CTAG C^TAG
1803 CTAG
BglI 56 GCCTTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
165 GCCCACCCGGC
BmyI 36 GAGCTC G[AGT]GC[ACT]^C
1254 GGGCAC
BpmI 71 CTCCAG CTGGAG(16/14)
304 CTGGAG
BsgI 696 CTGCAC GTGCAG(16/14)
628 GTGCAG
BsiEI 172 CGGCCG CG[GA][CT]^CG
324 CGACCG
BsmI 837 GAATGC GAATGC(1/-1)
1715 GCATTC
Bsp1286I 36 GAGCTC G[AGT]GC[ACT]^C
1254 GGGCAC
Bsp1407I 1049 TGTACA T^GTACA
1165 TGTACA
BsrGI 1049 TGTACA T^GTACA
1165 TGTACA
BstF5I 531 GGATG GGATG(2/0)
1206 GGATG
BstYI 1242 GGATCT [GA]^GATC[CT]
1317 AGATCC
DraI 520 TTTAAA TTT^AAA
1706 TTTAAA
EcoRI 691 GAATTC G^AATTC
931 GAATTC
FokI 531 GGATG GGATG(9/13)
1206 GGATG
HaeII 94 GGCGCC [GA]GCGC^[CT]
1121 AGCGCC
HgaI 761 GACGC GACGC(5/10)
108 GCGTC
MaeI 1605 CTAG C^TAG
1803 CTAG
MaeII 412 ACGT A^CGT
1353 ACGT
MaeIII 416 GTGAC ^GT[ACGT]AC
1471 GTCAC
MflI 1242 GGATCT [GA]^GATC[CT]
1317 AGATCC
NciI 170 CCCGG CC^[GC]GG
327 CCGGG
SphI 764 GCATGC GCATG^C
1455 GCATGC
SspBI 1049 TGTACA T^GTACA
1165 TGTACA
SspI 806 AATATT AAT^ATT
1739 AATATT
Tsp45I 416 GTGAC ^GT[GC]AC
1471 GTCAC
XhoII 1242 GGATCT [GA]^GATC[CT]
1317 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI BanI BbvI BsaJI
BsiYI BslI BsmFI BsrI BssKI
BstNI BstUI Cac8I CfoI DdeI
DpnII DsaI EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PvuII RsaI Sau3AI
ScrFI SfaNI TfiI Tru9I TseI
Tsp509I TspRI