##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI NaeI
NarI NcoI NgoMIV NheI NotI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Van91I XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 761 GTAGAC GT^[AC][GT]AC
AhdI 773 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 830 CCTGAGG CC^T[ACGT]AGG
ApaI 1856 GGGCCC GGGCC^C
AspEI 773 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 854 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1023 CTCGAG C^[CT]CG[GA]G
BamHI 904 GGATCC G^GATCC
BanI 1177 GGTGCC G^G[CT][GA]CC
BciVI 878 GTATCC GTATCC(6/5)
BsaI 948 GAGACC GGTCTC(1/5)
BsiEI 685 CGATCG CG[GA][CT]^CG
BsmI 693 GCATTC GAATGC(1/-1)
BsoBI 1023 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1371 TGTACA T^GTACA
BspMI 11 GCAGGT ACCTGC(4/8)
BsrBI 1208 GAGCGG CCGCTC(-3/-3)
BsrGI 1371 TGTACA T^GTACA
BstAPI 53 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 862 CCAGCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 830 CCTGAGG CC^T[ACGT]AGG
DrdI 665 GACTTCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 773 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 830 CCTGAGG CC^T[ACGT]AGG
EcoRI 557 GAATTC G^AATTC
HinfI 377 GACTC G^A[ACGT]TC
MaeII 282 ACGT A^CGT
NdeI 1642 CATATG CA^TATG
NruI 265 TCGCGA TCG^CGA
NspI 1152 GCATGT [GA]CATG^[CT]
PaeR7I 1023 CTCGAG C^TCGAG
PflFI 854 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 377 GACTC GAGTC(4/5)
PshAI 239 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1851 CTGCAG CTGCA^G
PvuI 685 CGATCG CGAT^CG
SacI 416 GAGCTC GAGCT^C
SapI 1094 GAAGAGC GCTCTTC(1/4)
ScaI 1407 AGTACT AGT^ACT
SspBI 1371 TGTACA T^GTACA
Tsp45I 1490 GTGAC ^GT[GC]AC
Tth111I 854 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 697 TCTAGA T^CTAGA
XcmI 862 CCAGCAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1023 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 98 CGCG CG^CG
266 CGCG
AcsI 206 AAATTT [GA]^AATT[CT]
557 GAATTC
ApoI 206 AAATTT [GA]^AATT[CT]
557 GAATTC
BglI 44 GCCGGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1787 GCCCAGGAGGC
BlpI 1192 GCTCAGC GC^T[ACGT]AGC
1361 GCTGAGC
Bpu1102I 1192 GCTCAGC GC^T[ACGT]AGC
1361 GCTGAGC
BsgI 1293 CTGCAC GTGCAG(16/14)
312 GTGCAG
BsrDI 580 CATTGC GCAATG(2/0)
467 GCAATG
BstUI 98 CGCG CG^CG
266 CGCG
BstYI 639 AGATCC [GA]^GATC[CT]
904 GGATCC
CelII 1192 GCTCAGC GC^T[ACGT]AGC
1361 GCTGAGC
EaeI 544 TGGCCG [CT]^GGCC[GA]
1757 CGGCCA
EcoNI 670 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1528 CCTTGTGGAGG
EcoT14I 225 CCAAGG C^C[AT][AT]GG
933 CCTTGG
HgaI 690 GACGC GACGC(5/10)
1131 GACGC
HindIII 383 AAGCTT A^AGCTT
1325 AAGCTT
MaeIII 1402 GTTAC ^GT[ACGT]AC
1490 GTGAC
MfeI 1653 CAATTG C^AATTG
1894 CAATTG
MflI 639 AGATCC [GA]^GATC[CT]
904 GGATCC
MseI 489 TTAA T^TAA
1629 TTAA
MslI 1042 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1198 CATTGGGGTG
MunI 1653 CAATTG C^AATTG
1894 CAATTG
MvnI 98 CGCG CG^CG
266 CGCG
PpuMI 184 GGGTCCT [GA]G^G[AT]CC[CT]
1826 AGGACCC
PvuII 179 CAGCTG CAG^CTG
893 CAGCTG
StuI 106 AGGCCT AGG^CCT
930 AGGCCT
StyI 225 CCAAGG C^C[AT][AT]GG
933 CCTTGG
Tru9I 489 TTAA T^TAA
1629 TTAA
XhoII 639 AGATCC [GA]^GATC[CT]
904 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TseI Tsp509I
TspRI TthHB8I