##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BbsI BbvCI
BcgI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BsrBI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Tsp45I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 812 GACGCC G[GA]^CG[CT]C
AflII 1589 CTTAAG C^TTAAG
AflIII 1331 ACATGT A^C[GA][CT]GT
Alw44I 130 GTGCAC G^TGCAC
ApaLI 130 GTGCAC G^TGCAC
AseI 1483 ATTAAT AT^TAAT
AsnI 1483 ATTAAT AT^TAAT
BamHI 27 GGATCC G^GATCC
BanII 896 GAGCCC G[GA]GC[CT]^C
BclI 271 TGATCA T^GATCA
BcnI 923 CCCGG CC^[GC]GG
BfrI 1589 CTTAAG C^TTAAG
BglI 1186 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 253 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 812 GACGCC G[GA]^CG[CT]C
BsmFI 7 GGGAC GGGAC(10/14)
Bsp1407I 1669 TGTACA T^GTACA
BspLU11I 1331 ACATGT A^CATGT
BspMI 1638 GCAGGT ACCTGC(4/8)
BsrFI 845 GCCGGC [GA]^CCGG[CT]
BsrGI 1669 TGTACA T^GTACA
BssSI 749 CACGAG CACGAG(-5/-1)
BstYI 27 GGATCC [GA]^GATC[CT]
Cfr10I 845 GCCGGC [GA]^CCGG[CT]
EaeI 1000 TGGCCG [CT]^GGCC[GA]
EarI 229 GAAGAG CTCTTC(1/4)
FbaI 271 TGATCA T^GATCA
Hin1I 812 GACGCC G[GA]^CG[CT]C
HindIII 1056 AAGCTT A^AGCTT
Ksp632I 229 GAAGAG CTCTTC(1/4)
MaeII 10 ACGT A^CGT
MamI 253 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 27 GGATCC [GA]^GATC[CT]
NaeI 845 GCCGGC GCC^GGC
NciI 923 CCCGG CC^[GC]GG
NcoI 516 CCATGG C^CATGG
NgoMIV 845 GCCGGC G^CCGGC
PshBI 1483 ATTAAT AT^TAAT
PvuII 803 CAGCTG CAG^CTG
SphI 1578 GCATGC GCATG^C
SspBI 1669 TGTACA T^GTACA
XcmI 322 CCAAGCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 27 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 808 GTAC GT^AC
1670 GTAC
AspHI 130 GTGCAC G[AT]GC[AT]^C
1074 GTGCTC
AvaII 468 GGACC G^G[AT]CC
772 GGACC
BciVI 521 GGATAC GTATCC(6/5)
1559 GTATCC
BsaI 43 GAGACC GGTCTC(1/5)
420 GAGACC
BsiHKAI 130 GTGCAC G[AT]GC[AT]^C
1074 GTGCTC
BspHI 301 TCATGA T^CATGA
1567 TCATGA
BsrDI 390 GCAATG GCAATG(2/0)
840 GCAATG
DraI 1315 TTTAAA TTT^AAA
1838 TTTAAA
DsaI 23 CCGTGG C^C[GA][CT]GG
516 CCATGG
EcoT14I 366 CCAAGG C^C[AT][AT]GG
516 CCATGG
HaeII 729 GGCGCT [GA]GCGC^[CT]
1194 GGCGCT
HapII 846 CCGG C^CGG
924 CCGG
HpaII 846 CCGG C^CGG
924 CCGG
HphI 646 GGTGA GGTGA(8/7)
171 TCACC
MaeIII 395 GTTAC ^GT[ACGT]AC
1374 GTTAC
MslI 380 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
399 CACTGCCATG
MspI 846 CCGG C^CGG
924 CCGG
NspI 1331 ACATGT [GA]CATG^[CT]
1578 GCATGC
RcaI 301 TCATGA T^CATGA
1567 TCATGA
RsaI 808 GTAC GT^AC
1670 GTAC
SmlI 491 CTCAAG C^T[CT][GA]AG
1589 CTTAAG
SspI 1801 AATATT AAT^ATT
1826 AATATT
StyI 366 CCAAGG C^C[AT][AT]GG
516 CCATGG
TaqI 418 TCGA T^CGA
798 TCGA
TfiI 208 GATTC G^A[AT]TC
444 GAATC
TthHB8I 418 TCGA T^CGA
798 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccI AciI AcsI AluI AlwI
ApoI BbvI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HgaI HhaI
HinP1I HinfI ItaI MaeI MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I TseI Tsp509I TspRI