##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AviII AvrII BbeI BbvCI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FauI FseI FspI HgaI Hin1I
KasI KpnI KspI MamI MluI
MroI MspA1I MvnI NarI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AflII 1223 CTTAAG C^TTAAG
AhdI 1748 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1507 GTGCAC G^TGCAC
ApaLI 1507 GTGCAC G^TGCAC
Asp700I 1240 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1748 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 962 CCCGAG C^[CT]CG[GA]G
BalI 45 TGGCCA TGG^CCA
BamHI 207 GGATCC G^GATCC
BanI 737 GGTGCC G^G[CT][GA]CC
BanII 106 GAGCTC G[GA]GC[CT]^C
BbrPI 1990 CACGTG CAC^GTG
BbsI 1839 GAAGAC GAAGAC(2/6)
BcgI 730 CGATGGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 478 GGATAC GTATCC(6/5)
BclI 549 TGATCA T^GATCA
BcnI 1009 CCGGG CC^[GC]GG
BfrI 1223 CTTAAG C^TTAAG
BglII 1044 AGATCT A^GATCT
BpuAI 1839 GAAGAC GAAGAC(2/6)
BsaAI 1990 CACGTG [CT]AC^GT[GA]
BsaWI 1524 TCCGGT [AT]^CCGG[AT]
BsgI 1629 GTGCAG GTGCAG(16/14)
BsoBI 962 CCCGAG C^[CT]CG[GA]G
BsrDI 641 GCAATG GCAATG(2/0)
BsrFI 1284 GCCGGC [GA]^CCGG[CT]
BstXI 741 CCAAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1284 GCCGGC [GA]^CCGG[CT]
DraIII 1501 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 45 TGGCCA [CT]^GGCC[GA]
Eam1105I 1748 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1896 ATGCAT ATGCA^T
FbaI 549 TGATCA T^GATCA
HaeII 12 AGCGCC [GA]GCGC^[CT]
HpaI 1916 GTTAAC GTT^AAC
MfeI 1751 CAATTG C^AATTG
MluNI 45 TGGCCA TGG^CCA
MscI 45 TGGCCA TGG^CCA
MunI 1751 CAATTG C^AATTG
NaeI 1284 GCCGGC GCC^GGC
NciI 1009 CCGGG CC^[GC]GG
NcoI 42 CCATGG C^CATGG
NgoMIV 1284 GCCGGC G^CCGGC
NheI 1463 GCTAGC G^CTAGC
NsiI 1896 ATGCAT ATGCA^T
PleI 673 GAGTC GAGTC(4/5)
PmaCI 1990 CACGTG CAC^GTG
PmlI 1990 CACGTG CAC^GTG
SacI 106 GAGCTC GAGCT^C
ScaI 1023 AGTACT AGT^ACT
SexAI 1566 ACCAGGT A^CC[AT]GGT
SspI 1764 AATATT AAT^ATT
TaqI 691 TCGA T^CGA
TthHB8I 691 TCGA T^CGA
XmnI 1240 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 21 CCGC CCGC(-3/-1)
55 CCGC
AlwNI 28 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
671 CAGAGTCTG
AspHI 106 GAGCTC G[AT]GC[AT]^C
1507 GTGCAC
BfaI 971 CTAG C^TAG
1464 CTAG
BmyI 106 GAGCTC G[AGT]GC[ACT]^C
1507 GTGCAC
BsaI 398 GAGACC GGTCTC(1/5)
766 GGTCTC
BseRI 116 GAGGAG GAGGAG(10/8)
119 GAGGAG
BsiHKAI 106 GAGCTC G[AT]GC[AT]^C
1507 GTGCAC
Bsp1286I 106 GAGCTC G[AGT]GC[ACT]^C
1507 GTGCAC
BspLU11I 312 ACATGT A^CATGT
1920 ACATGT
Cac8I 1284 GCCGGC GC[ACGT]^[ACGT]GC
1463 GCTAGC
CfoI 2 GCGC GCG^C
13 GCGC
DsaI 42 CCATGG C^C[GA][CT]GG
1119 CCGTGG
EarI 113 GAAGAG CTCTTC(1/4)
1157 GAAGAG
EcoRI 408 GAATTC G^AATTC
917 GAATTC
HhaI 2 GCGC GCG^C
13 GCGC
HinP1I 2 GCGC G^CGC
13 GCGC
HincII 505 GTCAAC GT[CT]^[GA]AC
1916 GTTAAC
HindII 505 GTCAAC GT[CT]^[GA]AC
1916 GTTAAC
HindIII 1619 AAGCTT A^AGCTT
1662 AAGCTT
Ksp632I 113 GAAGAG CTCTTC(1/4)
1157 GAAGAG
MaeI 971 CTAG C^TAG
1464 CTAG
MaeII 798 ACGT A^CGT
1991 ACGT
MslI 96 CATATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1414 CACATATGTG
NspI 312 ACATGT [GA]CATG^[CT]
1920 ACATGT
PflMI 27 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
359 CCATTGACTGG
PstI 1295 CTGCAG CTGCA^G
1575 CTGCAG
SmlI 761 CTCAAG C^T[CT][GA]AG
1223 CTTAAG
Van91I 27 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
359 CCATTGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
ApoI AvaII BbvI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfcI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII