##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AlwNI Aor51HI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BamHI BbeI BbvCI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspLU11I BspMI BsrFI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraI DrdI Eco47III EcoO65I
EcoRV FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccIII 1419 TCCGGA T^CCGGA
AfaI 211 GTAC GT^AC
AflIII 784 ACGTGT A^C[GA][CT]GT
AhdI 915 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 310 GTGCAC G^TGCAC
AlwI 361 GATCC GGATC(4/5)
AocI 813 CCTGAGG CC^T[ACGT]AGG
ApaI 1308 GGGCCC GGGCC^C
ApaLI 310 GTGCAC G^TGCAC
AspEI 915 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 769 GGTGCC G^G[CT][GA]CC
BbrPI 467 CACGTG CAC^GTG
BbsI 1085 GTCTTC GAAGAC(2/6)
BcnI 195 CCGGG CC^[GC]GG
BglI 378 GCCAGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1250 AGATCT A^GATCT
BpmI 257 CTGGAG CTGGAG(16/14)
BpuAI 1085 GTCTTC GAAGAC(2/6)
BsaBI 658 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1419 TCCGGA [AT]^CCGG[AT]
BseAI 1419 TCCGGA T^CCGGA
BsgI 535 CTGCAC GTGCAG(16/14)
BsiEI 244 CGGCCG CG[GA][CT]^CG
BspEI 1419 TCCGGA T^CCGGA
BspHI 1482 TCATGA T^CATGA
BssSI 206 CTCGTG CACGAG(-5/-1)
BstXI 699 CCAGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1250 AGATCT [GA]^GATC[CT]
Bsu36I 813 CCTGAGG CC^T[ACGT]AGG
EagI 244 CGGCCG C^GGCCG
Eam1105I 915 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 181 CTCTTC CTCTTC(1/4)
EclXI 244 CGGCCG C^GGCCG
Eco52I 244 CGGCCG C^GGCCG
Eco81I 813 CCTGAGG CC^T[ACGT]AGG
EcoRI 1375 GAATTC G^AATTC
EcoT22I 1494 ATGCAT ATGCA^T
HaeII 73 GGCGCT [GA]GCGC^[CT]
HgaI 224 GACGC GACGC(5/10)
Ksp632I 181 CTCTTC CTCTTC(1/4)
KspI 141 CCGCGG CCGC^GG
MamI 658 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1250 AGATCT [GA]^GATC[CT]
MroI 1419 TCCGGA T^CCGGA
NciI 195 CCGGG CC^[GC]GG
NsiI 1494 ATGCAT ATGCA^T
NspI 568 GCATGT [GA]CATG^[CT]
PacI 1105 TTAATTAA TTAAT^TAA
PflMI 1195 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1062 GACTC GAGTC(4/5)
PmaCI 467 CACGTG CAC^GTG
PmlI 467 CACGTG CAC^GTG
PpuMI 849 AGGACCC [GA]G^G[AT]CC[CT]
PstI 154 CTGCAG CTGCA^G
RcaI 1482 TCATGA T^CATGA
RsaI 211 GTAC GT^AC
SacII 141 CCGCGG CCGC^GG
SfcI 154 CTGCAG C^T[GA][CT]AG
SmlI 1352 CTTGAG C^T[CT][GA]AG
SspI 1079 AATATT AAT^ATT
Van91I 1195 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1250 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 10 GACGTC GACGT^C
602 GACGTC
AcsI 1375 GAATTC [GA]^AATT[CT]
1469 AAATTC
ApoI 1375 GAATTC [GA]^AATT[CT]
1469 AAATTC
Asp700I 322 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1427 GAAGCATTTC
AspHI 310 GTGCAC G[AT]GC[AT]^C
1415 GTGCTC
AvaII 503 GGTCC G^G[AT]CC
850 GGACC
BalI 676 TGGCCA TGG^CCA
696 TGGCCA
BcgI 780 CGATACGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
597 GCATGGACGTCG
BsaAI 467 CACGTG [CT]AC^GT[GA]
783 TACGTG
BsiHKAI 310 GTGCAC G[AT]GC[AT]^C
1415 GTGCTC
BsrBI 203 CCGCTC CCGCTC(-3/-3)
239 CCGCTC
BsrDI 860 CATTGC GCAATG(2/0)
943 CATTGC
BssHII 2 GCGCGC G^CGCGC
4 GCGCGC
DpnII 361 GATC ^GATC
1251 GATC
DraII 849 AGGACCC [GA]G^G[ACGT]CC[CT]
1307 AGGGCCC
DraIII 353 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
804 CACAGTGTG
EcoO109I 849 AGGACCC [GA]G^G[ACGT]CC[CT]
1307 AGGGCCC
MboI 361 GATC ^GATC
1251 GATC
MluNI 676 TGGCCA TGG^CCA
696 TGGCCA
MscI 676 TGGCCA TGG^CCA
696 TGGCCA
NcoI 489 CCATGG C^CATGG
1312 CCATGG
NdeII 361 GATC ^GATC
1251 GATC
PvuII 288 CAGCTG CAG^CTG
719 CAGCTG
Sau3AI 361 GATC ^GATC
1251 GATC
SfaNI 67 GATGC GCATC(5/9)
904 GCATC
TfiI 620 GAATC G^A[AT]TC
967 GATTC
XmnI 322 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1427 GAAGCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcyI AluI BanII
BbvI BfaI BmyI BsaHI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DsaI
EaeI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NlaIII NlaIV
Sau96I ScrFI StyI Tru9I TseI
Tsp45I Tsp509I TspRI