##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AspI AvaII AviII
BalI BamHI BbvCI BcgI BclI
BfrI BglI BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I CelII ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MfeI MluI MluNI MroI MscI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SalI
SbfI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcyI 190 GGCGCC G[GA]^CG[CT]C
AflIII 1285 ACATGT A^C[GA][CT]GT
Alw44I 1373 GTGCAC G^TGCAC
AlwI 1465 GGATC GGATC(4/5)
Aor51HI 1057 AGCGCT AGC^GCT
ApaI 843 GGGCCC GGGCC^C
ApaLI 1373 GTGCAC G^TGCAC
AvrII 684 CCTAGG C^CTAGG
BbeI 190 GGCGCC GGCGC^C
BbrPI 160 CACGTG CAC^GTG
BciVI 876 GGATAC GTATCC(6/5)
BglII 1646 AGATCT A^GATCT
BlnI 684 CCTAGG C^CTAGG
BpmI 992 CTGGAG CTGGAG(16/14)
BsaAI 160 CACGTG [CT]AC^GT[GA]
BsaBI 1148 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 190 GGCGCC G[GA]^CG[CT]C
BsaI 44 GGTCTC GGTCTC(1/5)
BsgI 1022 GTGCAG GTGCAG(16/14)
BsmAI 45 GTCTC GTCTC(1/5)
BsmI 1091 GAATGC GAATGC(1/-1)
BspHI 605 TCATGA T^CATGA
BspLU11I 1285 ACATGT A^CATGT
BspMI 1494 ACCTGC ACCTGC(4/8)
BsrDI 198 CATTGC GCAATG(2/0)
BstEII 832 GGTTACC G^GT[ACGT]ACC
BstPI 832 GGTTACC G^GT[ACGT]ACC
EaeI 137 CGGCCA [CT]^GGCC[GA]
EarI 463 CTCTTC CTCTTC(1/4)
Eco47III 1057 AGCGCT AGC^GCT
EcoO65I 832 GGTTACC G^GT[ACGT]ACC
Hin1I 190 GGCGCC G[GA]^CG[CT]C
KasI 190 GGCGCC G^GCGCC
Ksp632I 463 CTCTTC CTCTTC(1/4)
KspI 9 CCGCGG CCGC^GG
MaeII 161 ACGT A^CGT
MamI 1148 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 190 GGCGCC GG^CGCC
NspI 1285 ACATGT [GA]CATG^[CT]
PflMI 69 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 160 CACGTG CAC^GTG
PmlI 160 CACGTG CAC^GTG
RcaI 605 TCATGA T^CATGA
SacI 460 GAGCTC GAGCT^C
SacII 9 CCGCGG CCGC^GG
SapI 462 GCTCTTC GCTCTTC(1/4)
ScaI 1351 AGTACT AGT^ACT
SfoI 190 GGCGCC GGC^GCC
SmaI 40 CCCGGG CCC^GGG
TaqI 1160 TCGA T^CGA
TthHB8I 1160 TCGA T^CGA
Van91I 69 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 858 TCTAGA T^CTAGA
XmaCI 40 CCCGGG C^CCGGG
XmaI 40 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 241 GTAGAC GT^[AC][GT]AC
667 GTAGAC
AcsI 346 GAATTT [GA]^AATT[CT]
1750 AAATTT
AfaI 1123 GTAC GT^AC
1352 GTAC
AocI 294 CCTTAGG CC^T[ACGT]AGG
331 CCTAAGG
ApoI 346 GAATTT [GA]^AATT[CT]
1750 AAATTT
Asp700I 972 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1208 GAAATAATTC
AvaI 40 CCCGGG C^[CT]CG[GA]G
1076 CCCGAG
BanI 190 GGCGCC G^G[CT][GA]CC
1270 GGTGCC
BanII 460 GAGCTC G[GA]GC[CT]^C
843 GGGCCC
BcnI 40 CCCGG CC^[GC]GG
41 CCGGG
BseRI 47 CTCCTC GAGGAG(10/8)
114 CTCCTC
BsoBI 40 CCCGGG C^[CT]CG[GA]G
1076 CCCGAG
BstAPI 93 GCAGTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1602 GCATGGTTTGC
BstYI 1465 GGATCT [GA]^GATC[CT]
1646 AGATCT
Bsu36I 294 CCTTAGG CC^T[ACGT]AGG
331 CCTAAGG
Cfr13I 843 GGGCC G^G[ACGT]CC
844 GGCCC
DpnII 1466 GATC ^GATC
1647 GATC
Eco81I 294 CCTTAGG CC^T[ACGT]AGG
331 CCTAAGG
FauI 18 CCCGC CCCGC(4/6)
179 GCGGG
HaeII 190 GGCGCC [GA]GCGC^[CT]
1057 AGCGCT
HgaI 787 GACGC GACGC(5/10)
28 GCGTC
MboI 1466 GATC ^GATC
1647 GATC
MflI 1465 GGATCT [GA]^GATC[CT]
1646 AGATCT
MspA1I 9 CCGCGG C[AC]G^C[GT]G
63 CCGCTG
NaeI 193 GCCGGC GCC^GGC
337 GCCGGC
NciI 40 CCCGG CC^[GC]GG
41 CCGGG
NcoI 74 CCATGG C^CATGG
839 CCATGG
NdeII 1466 GATC ^GATC
1647 GATC
NgoMIV 193 GCCGGC G^CCGGC
337 GCCGGC
RsaI 1123 GTAC GT^AC
1352 GTAC
Sau3AI 1466 GATC ^GATC
1647 GATC
Sau96I 843 GGGCC G^G[ACGT]CC
844 GGCCC
XhoII 1465 GGATCT [GA]^GATC[CT]
1646 AGATCT
XmnI 972 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1208 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
BbsI BbvI BfaI BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I DdeI
DsaI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboII MnlI MseI MslI MspI
MvaI MvnI MwoI NlaIII NlaIV
PleI PstI ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI