##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaII       AviII       
BalI        BamHI       BbvCI       BcgI        BclI        
BfrI        BglI        BlpI        Bpu1102I    BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmFI       
Bsp1407I    BspDI       BspEI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstXI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoNI       EcoO109I    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SalI        
SbfI        SexAI       SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AcyI          190  GGCGCC               G[GA]^CG[CT]C
AflIII       1285  ACATGT               A^C[GA][CT]GT
Alw44I       1373  GTGCAC               G^TGCAC
AlwI         1465  GGATC                GGATC(4/5)
Aor51HI      1057  AGCGCT               AGC^GCT
ApaI          843  GGGCCC               GGGCC^C
ApaLI        1373  GTGCAC               G^TGCAC
AvrII         684  CCTAGG               C^CTAGG
BbeI          190  GGCGCC               GGCGC^C
BbrPI         160  CACGTG               CAC^GTG
BciVI         876  GGATAC               GTATCC(6/5)
BglII        1646  AGATCT               A^GATCT
BlnI          684  CCTAGG               C^CTAGG
BpmI          992  CTGGAG               CTGGAG(16/14)
BsaAI         160  CACGTG               [CT]AC^GT[GA]
BsaBI        1148  GATTGTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         190  GGCGCC               G[GA]^CG[CT]C
BsaI           44  GGTCTC               GGTCTC(1/5)
BsgI         1022  GTGCAG               GTGCAG(16/14)
BsmAI          45  GTCTC                GTCTC(1/5)
BsmI         1091  GAATGC               GAATGC(1/-1)
BspHI         605  TCATGA               T^CATGA
BspLU11I     1285  ACATGT               A^CATGT
BspMI        1494  ACCTGC               ACCTGC(4/8)
BsrDI         198  CATTGC               GCAATG(2/0)
BstEII        832  GGTTACC              G^GT[ACGT]ACC
BstPI         832  GGTTACC              G^GT[ACGT]ACC
EaeI          137  CGGCCA               [CT]^GGCC[GA]
EarI          463  CTCTTC               CTCTTC(1/4)
Eco47III     1057  AGCGCT               AGC^GCT
EcoO65I       832  GGTTACC              G^GT[ACGT]ACC
Hin1I         190  GGCGCC               G[GA]^CG[CT]C
KasI          190  GGCGCC               G^GCGCC
Ksp632I       463  CTCTTC               CTCTTC(1/4)
KspI            9  CCGCGG               CCGC^GG
MaeII         161  ACGT                 A^CGT
MamI         1148  GATTGTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI          190  GGCGCC               GG^CGCC
NspI         1285  ACATGT               [GA]CATG^[CT]
PflMI          69  CCACACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         160  CACGTG               CAC^GTG
PmlI          160  CACGTG               CAC^GTG
RcaI          605  TCATGA               T^CATGA
SacI          460  GAGCTC               GAGCT^C
SacII           9  CCGCGG               CCGC^GG
SapI          462  GCTCTTC              GCTCTTC(1/4)
ScaI         1351  AGTACT               AGT^ACT
SfoI          190  GGCGCC               GGC^GCC
SmaI           40  CCCGGG               CCC^GGG
TaqI         1160  TCGA                 T^CGA
TthHB8I      1160  TCGA                 T^CGA
Van91I         69  CCACACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          858  TCTAGA               T^CTAGA
XmaCI          40  CCCGGG               C^CCGGG
XmaI           40  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccI          241  GTAGAC               GT^[AC][GT]AC
              667  GTAGAC              
AcsI          346  GAATTT               [GA]^AATT[CT]
             1750  AAATTT              
AfaI         1123  GTAC                 GT^AC
             1352  GTAC                
AocI          294  CCTTAGG              CC^T[ACGT]AGG
              331  CCTAAGG             
ApoI          346  GAATTT               [GA]^AATT[CT]
             1750  AAATTT              
Asp700I       972  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1208  GAAATAATTC          
AvaI           40  CCCGGG               C^[CT]CG[GA]G
             1076  CCCGAG              
BanI          190  GGCGCC               G^G[CT][GA]CC
             1270  GGTGCC              
BanII         460  GAGCTC               G[GA]GC[CT]^C
              843  GGGCCC              
BcnI           40  CCCGG                CC^[GC]GG
               41  CCGGG               
BseRI          47  CTCCTC               GAGGAG(10/8)
              114  CTCCTC              
BsoBI          40  CCCGGG               C^[CT]CG[GA]G
             1076  CCCGAG              
BstAPI         93  GCAGTTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1602  GCATGGTTTGC         
BstYI        1465  GGATCT               [GA]^GATC[CT]
             1646  AGATCT              
Bsu36I        294  CCTTAGG              CC^T[ACGT]AGG
              331  CCTAAGG             
Cfr13I        843  GGGCC                G^G[ACGT]CC
              844  GGCCC               
DpnII        1466  GATC                 ^GATC
             1647  GATC                
Eco81I        294  CCTTAGG              CC^T[ACGT]AGG
              331  CCTAAGG             
FauI           18  CCCGC                CCCGC(4/6)
              179  GCGGG               
HaeII         190  GGCGCC               [GA]GCGC^[CT]
             1057  AGCGCT              
HgaI          787  GACGC                GACGC(5/10)
               28  GCGTC               
MboI         1466  GATC                 ^GATC
             1647  GATC                
MflI         1465  GGATCT               [GA]^GATC[CT]
             1646  AGATCT              
MspA1I          9  CCGCGG               C[AC]G^C[GT]G
               63  CCGCTG              
NaeI          193  GCCGGC               GCC^GGC
              337  GCCGGC              
NciI           40  CCCGG                CC^[GC]GG
               41  CCGGG               
NcoI           74  CCATGG               C^CATGG
              839  CCATGG              
NdeII        1466  GATC                 ^GATC
             1647  GATC                
NgoMIV        193  GCCGGC               G^CCGGC
              337  GCCGGC              
RsaI         1123  GTAC                 GT^AC
             1352  GTAC                
Sau3AI       1466  GATC                 ^GATC
             1647  GATC                
Sau96I        843  GGGCC                G^G[ACGT]CC
              844  GGCCC               
XhoII        1465  GGATCT               [GA]^GATC[CT]
             1646  AGATCT              
XmnI          972  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1208  GAAATAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AspHI       
BbsI        BbvI        BfaI        BmyI        BpuAI       
BsaJI       BsiHKAI     BsiYI       BslI        Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      DdeI        
DsaI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeIII      
MboII       MnlI        MseI        MslI        MspI        
MvaI        MvnI        MwoI        NlaIII      NlaIV       
PleI        PstI        ScrFI       SfaNI       SfcI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI