##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AvaII AviII BamHI BanI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BsaAI BsaHI BsaI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspLU11I BsrBI BsrFI BssHII
BssSI BstBI BstEII BstPI BstUI
BstXI Bsu36I CfoI Cfr10I Cfr13I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII KasI KpnI
KspI MluI MslI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI Sau96I SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tth111I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 296 TCCGGA T^CCGGA
AspHI 995 GAGCTC G[AT]GC[AT]^C
AvrII 766 CCTAGG C^CTAGG
BalI 1786 TGGCCA TGG^CCA
BciVI 2104 GGATAC GTATCC(6/5)
BcnI 1366 CCGGG CC^[GC]GG
BglII 931 AGATCT A^GATCT
BlnI 766 CCTAGG C^CTAGG
BlpI 782 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 782 GCTCAGC GC^T[ACGT]AGC
BsaBI 74 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 296 TCCGGA [AT]^CCGG[AT]
BseAI 296 TCCGGA T^CCGGA
BsiHKAI 995 GAGCTC G[AT]GC[AT]^C
BsmAI 487 GTCTC GTCTC(1/5)
BspEI 296 TCCGGA T^CCGGA
BspHI 454 TCATGA T^CATGA
BspMI 1229 ACCTGC ACCTGC(4/8)
Bst1107I 1506 GTATAC GTA^TAC
BstZ17I 1506 GTATAC GTA^TAC
CelII 782 GCTCAGC GC^T[ACGT]AGC
DsaI 335 CCATGG C^C[GA][CT]GG
EaeI 1786 TGGCCA [CT]^GGCC[GA]
EcoRV 458 GATATC GAT^ATC
FauI 27 GCGGG CCCGC(4/6)
HgaI 898 GCGTC GACGC(5/10)
MamI 74 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1348 CAATTG C^AATTG
MluNI 1786 TGGCCA TGG^CCA
MroI 296 TCCGGA T^CCGGA
MscI 1786 TGGCCA TGG^CCA
MspA1I 1182 CAGCTG C[AC]G^C[GT]G
MunI 1348 CAATTG C^AATTG
NciI 1366 CCGGG CC^[GC]GG
NcoI 335 CCATGG C^CATGG
NheI 1069 GCTAGC G^CTAGC
NlaIV 268 GGAACC GG[ACGT]^[ACGT]CC
PflMI 2314 CCATTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1182 CAGCTG CAG^CTG
RcaI 454 TCATGA T^CATGA
SacI 995 GAGCTC GAGCT^C
SapI 85 GAAGAGC GCTCTTC(1/4)
ScaI 2052 AGTACT AGT^ACT
SphI 1408 GCATGC GCATG^C
TaqI 772 TCGA T^CGA
Tsp45I 215 GTCAC ^GT[GC]AC
TthHB8I 772 TCGA T^CGA
Van91I 2314 CCATTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 308 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1506 GTATAC GT^[AC][GT]AC
2204 GTAGAC
AciI 14 GCGG CCGC(-3/-1)
27 GCGG
AseI 1986 ATTAAT AT^TAAT
2255 ATTAAT
AsnI 1986 ATTAAT AT^TAAT
2255 ATTAAT
BanII 361 GAGCCC G[GA]GC[CT]^C
995 GAGCTC
BbsI 324 GTCTTC GAAGAC(2/6)
1267 GTCTTC
BmyI 361 GAGCCC G[AGT]GC[ACT]^C
995 GAGCTC
BpmI 1537 CTCCAG CTGGAG(16/14)
1915 CTGGAG
BpuAI 324 GTCTTC GAAGAC(2/6)
1267 GTCTTC
BseRI 240 GAGGAG GAGGAG(10/8)
992 GAGGAG
BsmFI 19 GGGAC GGGAC(10/14)
493 GGGAC
Bsp1286I 361 GAGCCC G[AGT]GC[ACT]^C
995 GAGCTC
Bsp1407I 643 TGTACA T^GTACA
1045 TGTACA
BsrGI 643 TGTACA T^GTACA
1045 TGTACA
BstAPI 1356 GCAATCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2061 GCAAAACATGC
DraI 1601 TTTAAA TTT^AAA
1994 TTTAAA
HaeIII 1787 GGCC GG^CC
2323 GGCC
HapII 297 CCGG C^CGG
1366 CCGG
HpaII 297 CCGG C^CGG
1366 CCGG
MaeII 721 ACGT A^CGT
805 ACGT
MspI 297 CCGG C^CGG
1366 CCGG
NspI 1408 GCATGC [GA]CATG^[CT]
2066 ACATGC
PshBI 1986 ATTAAT AT^TAAT
2255 ATTAAT
PstI 1171 CTGCAG CTGCA^G
1835 CTGCAG
SexAI 67 ACCTGGT A^CC[AT]GGT
2410 ACCTGGT
SmlI 584 CTCAAG C^T[CT][GA]AG
880 CTCAAG
SspBI 643 TGTACA T^GTACA
1045 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbvI BfaI BsaJI BsiYI
BslI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HincII HindII HinfI HpaI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII PleI
RsaI Sau3AI ScrFI SfaNI SfcI
SspI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII XmnI