##################################
# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsiEI BsiWI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraI
DraII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HaeIII Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tth111I Van91I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 10 enzymes) #
###################################
AcsI 3954 AAATTT [GA]^AATT[CT]
AlwNI 169 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 3954 AAATTT [GA]^AATT[CT]
AvaII 3903 GGTCC G^G[AT]CC
BsrDI 3960 CATTGC GCAATG(2/0)
Cfr13I 3903 GGTCC G^G[ACGT]CC
MseI 3941 TTAA T^TAA
Sau96I 3903 GGTCC G^G[ACGT]CC
Tru9I 3941 TTAA T^TAA
Tsp509I 3955 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 2 enzymes) #
###################################
AccI 468 GTCTAC GT^[AC][GT]AC
2748 GTCTAC
MaeII 350 ACGT A^CGT
806 ACGT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AfaI AluI AlwI Asp700I AspHI
AvaI BanII BbsI BbvI BclI
BglII BmyI BpuAI BsaBI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmI BsoBI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI CfoI
DdeI DpnII DraIII EcoRII FbaI
Fnu4HI FokI HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MamI MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIV PaeR7I PleI PvuII
RsaI Sau3AI ScrFI SexAI SfaNI
SmlI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XhoI XhoII XmnI