##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I AlwNI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BlnI BpuAI BsaAI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I FseI FspI HapII HgaI
HpaII KasI KpnI KspI MluI
MluNI MroI MscI MspA1I MspI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1241 GACGTC GACGT^C
AccI 1394 GTCTAC GT^[AC][GT]AC
AcyI 1241 GACGTC G[GA]^CG[CT]C
AflIII 795 ACATGT A^C[GA][CT]GT
Aor51HI 590 AGCGCT AGC^GCT
ApaI 1443 GGGCCC GGGCC^C
AspI 1482 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1495 GGCACC G^G[CT][GA]CC
BanII 1443 GGGCCC G[GA]GC[CT]^C
BclI 76 TGATCA T^GATCA
BglII 1363 AGATCT A^GATCT
BlpI 15 GCTAAGC GC^T[ACGT]AGC
BmyI 1443 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 15 GCTAAGC GC^T[ACGT]AGC
BsaHI 1241 GACGTC G[GA]^CG[CT]C
Bsp1286I 1443 GGGCCC G[AGT]GC[ACT]^C
BspLU11I 795 ACATGT A^CATGT
BsrBI 1439 GAGCGG CCGCTC(-3/-3)
BstEII 343 GGTGACC G^GT[ACGT]ACC
BstPI 343 GGTGACC G^GT[ACGT]ACC
BstYI 1363 AGATCT [GA]^GATC[CT]
CelII 15 GCTAAGC GC^T[ACGT]AGC
CfoI 591 GCGC GCG^C
Eco47III 590 AGCGCT AGC^GCT
EcoO65I 343 GGTGACC G^GT[ACGT]ACC
EcoRI 188 GAATTC G^AATTC
EcoRV 1503 GATATC GAT^ATC
FauI 1441 GCGGG CCCGC(4/6)
FbaI 76 TGATCA T^GATCA
HaeII 590 AGCGCT [GA]GCGC^[CT]
HhaI 591 GCGC GCG^C
Hin1I 1241 GACGTC G[GA]^CG[CT]C
HinP1I 591 GCGC G^CGC
HincII 1732 GTTAAC GT[CT]^[GA]AC
HindII 1732 GTTAAC GT[CT]^[GA]AC
HpaI 1732 GTTAAC GTT^AAC
MaeII 1242 ACGT A^CGT
MfeI 104 CAATTG C^AATTG
MflI 1363 AGATCT [GA]^GATC[CT]
MunI 104 CAATTG C^AATTG
PflFI 1482 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 698 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1486 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 808 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 274 GAAGAGC GCTCTTC(1/4)
ScaI 1431 AGTACT AGT^ACT
SspI 1631 AATATT AAT^ATT
TaqI 820 TCGA T^CGA
Tth111I 1482 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 820 TCGA T^CGA
Van91I 698 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1087 TCTAGA T^CTAGA
XhoII 1363 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 955 GATCC GGATC(4/5)
178 GGATC
AocI 673 CCTAAGG CC^T[ACGT]AGG
1490 CCTTAGG
AvaII 568 GGACC G^G[AT]CC
1487 GGTCC
BglI 618 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
985 GCCTAGAGGGC
BpmI 98 CTGGAG CTGGAG(16/14)
1427 CTGGAG
BsaBI 74 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
955 GATCCAAATC
BseRI 5 GAGGAG GAGGAG(10/8)
334 GAGGAG
BsmAI 1450 GAGAC GTCTC(1/5)
1480 GAGAC
Bsu36I 673 CCTAAGG CC^T[ACGT]AGG
1490 CCTTAGG
DraI 629 TTTAAA TTT^AAA
1264 TTTAAA
DraII 1443 GGGCCCT [GA]G^G[ACGT]CC[CT]
1486 AGGTCCT
EarI 274 GAAGAG CTCTTC(1/4)
1136 GAAGAG
Eco81I 673 CCTAAGG CC^T[ACGT]AGG
1490 CCTTAGG
EcoNI 409 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
673 CCTAAGGCAGG
EcoO109I 1443 GGGCCCT [GA]G^G[ACGT]CC[CT]
1486 AGGTCCT
EcoT14I 537 CCAAGG C^C[AT][AT]GG
1384 CCTTGG
EcoT22I 1159 ATGCAT ATGCA^T
1673 ATGCAT
Ksp632I 274 GAAGAG CTCTTC(1/4)
1136 GAAGAG
MaeIII 114 GTCAC ^GT[ACGT]AC
344 GTGAC
MamI 74 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
955 GATCCAAATC
MslI 1592 CACTGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1694 CATTTGTATG
NsiI 1159 ATGCAT ATGCA^T
1673 ATGCAT
NspI 795 ACATGT [GA]CATG^[CT]
1675 GCATGT
PleI 171 GAGTC GAGTC(4/5)
337 GAGTC
PstI 28 CTGCAG CTGCA^G
1050 CTGCAG
SfaNI 919 GCATC GCATC(5/9)
1289 GCATC
SmlI 869 CTTGAG C^T[CT][GA]AG
1375 CTTGAG
StuI 540 AGGCCT AGG^CCT
885 AGGCCT
StyI 537 CCAAGG C^C[AT][AT]GG
1384 CCTTGG
TfiI 584 GAATC G^A[AT]TC
1046 GAATC
Tsp45I 114 GTCAC ^GT[GC]AC
344 GTGAC
XcmI 201 CCAAATACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1068 CCACATTTGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BfaI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI MaeI MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfcI Tru9I TseI Tsp509I TspRI